LOCUS       sequence-313981-        2395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0412
FEATURES             Location/Qualifiers
     source          1..2395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(129..223)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(244..903)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     818..837
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1065..1083
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1087..1130)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1501..1558
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1582..1599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1753..2341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1833..1852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
       61 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg
      121 aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
      181 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
      241 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
      301 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
      361 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
      421 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
      481 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
      541 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
      601 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
      661 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
      721 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
      781 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
      841 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
      901 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
      961 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac
     1021 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     1081 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgg
     1141 tctcaggagg gaggcgtgtc gttttagtga ggatgagcta agcacatacg tcagccgatg
     1201 ctgatcttgc ctgccttgaa atttcgggaa acctcctcga aaactggtac ttgtgtacag
     1261 ggctcactct ctctgccgac agtggtccca aaagggctca ctgctaggag gaccgatggc
     1321 tttgtcaaaa gctaaaaaag atgatgctgc tgatctttcc atcaacaaat aatccaagta
     1381 agcctcacat gtctgacgta agggatgacg cacatgctga tcttgcctgc cctatataag
     1441 gttttgctat tcattgaaag cagtagtgac tgatttgtat atatactcga gaccctgcag
     1501 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
     1561 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1621 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1681 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1741 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1801 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1861 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1921 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1981 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2041 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2101 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2161 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2221 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2281 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2341 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actca
//
