LOCUS       sequence-313983-        2519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0095
FEATURES             Location/Qualifiers
     source          1..2519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     526..545
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     773..791
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(795..838)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1333..1390
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1414..1431)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1585..2173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1665..1684)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2356..2450)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2471..2519,1..611))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
       61 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
      121 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg
      181 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
      241 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
      301 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
      361 atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg
      421 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
      481 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
      541 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
      601 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
      661 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactcg
      721 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
      781 tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg
      841 gaattcggtc tcaggagtgg aagattcaga aaatctccat caacaaataa tccaagtaag
      901 gattaatgga ttgatcaaca tccttaccgc tatgggtaag attgatgaaa agtcaaaaac
      961 aaaaatcaat tatgcacacc agcatgtgtt gatcaccagc tattgtggga caccaatttc
     1021 gtccacagac atcaacatct tatcgtcctt tgaagataag ataataatgt tgaagataag
     1081 agtgggagcc accactaaaa cattgctttg tcaaaagcta aaaaagatga tgcccgacag
     1141 ccacttgtgt gaagcatgtg aagccggtcc ctccactaag aaaattagtg aagcatcttc
     1201 cagtggtccc tccactcaca gctcaatcag tgagcaacag gacgaaggaa atgacgtaag
     1261 ccatgacgtc taatcccaca agaatttcct tatataagga acacaaatca gaaggtactc
     1321 gagaccctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa
     1381 accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt
     1441 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1501 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1561 taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa
     1621 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1681 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1741 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1801 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1861 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1921 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1981 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     2041 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2101 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2161 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2221 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2281 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2341 cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     2401 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     2461 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagc
//
