LOCUS       sequence-313985-        5706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-CSF1R
FEATURES             Location/Qualifiers
     source          1..5706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3017..3116)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3133..3152)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3134..3152)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3157..3173)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3157..3173)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3604..4395
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4488..5076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4977..4996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5230..5247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5406..5433)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5525..5611)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5660..5682
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5674..5691
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5675..5691
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgggcccagg agttctgctg
      121 ctcctgctgg tggccacagc ttggcatggt cagggaatcc cagtgataga gcccagtgtc
      181 cctgagctgg tcgtgaagcc aggagcaacg gtgaccttgc gatgtgtggg caatggcagc
      241 gtggaatggg atggcccccc atcacctcac tggaccctgt actctgatgg ctccagcagc
      301 atcctcagca ccaacaacgc taccttccaa aacacgggga cctatcgctg cactgagcct
      361 ggagaccccc tgggaggcag cgccgccatc cacctctatg tcaaagaccc tgcccggccc
      421 tggaacgtgc tagcacagga ggtggtcgtg ttcgaggacc aggacgcact actgccctgt
      481 ctgctcacag acccggtgct ggaagcaggc gtctcgctgg tgcgtgtgcg tggccggccc
      541 ctcatgcgcc acaccaacta ctccttctcg ccctggcatg gcttcaccat ccacagggcc
      601 aagttcattc agagccagga ctatcaatgc agtgccctga tgggtggcag gaaggtgatg
      661 tccatcagca tccggctgaa agtgcagaaa gtcatcccag ggcccccagc cttgacactg
      721 gtgcctgcag agctggtgcg gattcgaggg gaggctgccc agatcgtgtg ctcagccagc
      781 agcgttgatg ttaactttga tgtcttcctc caacacaaca acactaagct cgcaatccct
      841 caacaatctg actttcataa taaccgttac caaaaagtcc tgaccctcaa cctcgatcaa
      901 gtagatttcc aacatgccgg caactactcc tgcgtggcca gcaacgtgca gggcaagcac
      961 tccacctcca tgttcttccg ggtggtagag agtgcctact tgaacttgag ctctgagcag
     1021 aacctcatcc aggaggtgac cgtgggggag gggctcaacc tcaaagtcat ggtggaggcc
     1081 tacccaggcc tgcaaggttt taactggacc tacctgggac ccttttctga ccaccagcct
     1141 gagcccaagc ttgctaatgc taccaccaag gacacataca ggcacacctt caccctctct
     1201 ctgccccgcc tgaagccctc tgaggctggc cgctactcct tcctggccag aaacccagga
     1261 ggctggagag ctctgacgtt tgagctcacc cttcgatacc ccccagaggt aagcgtcata
     1321 tggacattca tcaacggctc tggcaccctt ttgtgtgctg cctctgggta cccccagccc
     1381 aacgtgacat ggctgcagtg cagtggccac actgataggt gtgatgaggc ccaagtgctg
     1441 caggtctggg atgacccata ccctgaggtc ctgagccagg agcccttcca caaggtgacg
     1501 gtgcagagcc tgctgactgt tgagacctta gagcacaacc aaacctacga gtgcagggcc
     1561 cacaacagcg tggggagtgg ctcctgggcc ttcataccca tctctgcagg agcccacacg
     1621 catcccccgg atgagttcct cttcacacca gtggtggtcg cctgcatgtc catcatggcc
     1681 ttgctgctgc tgctgctcct gctgctattg tacaagtata agcagaagcc caagtaccag
     1741 gtccgctgga agatcatcga gagctatgag ggcaacagtt atactttcat cgaccccacg
     1801 cagctgcctt acaacgagaa gtgggagttc ccccggaaca acctgcagtt tggtaagacc
     1861 ctcggagctg gagcctttgg gaaggtggtg gaggccacgg cctttggtct gggcaaggag
     1921 gatgctgtcc tgaaggtggc tgtgaagatg ctgaagtcca cggcccatgc tgatgagaag
     1981 gaggccctca tgtccgagct gaagatcatg agccacctgg gccagcacga gaacatcgtc
     2041 aaccttctgg gagcctgtac ccatggaggc cctgtactgg tcatcacgga gtactgttgc
     2101 tatggcgacc tgctcaactt tctgcgaagg aaggctgagg ccatgctggg acccagcctg
     2161 agccccggcc aggaccccga gggaggcgtc gactataaga acatccacct cgagaagaaa
     2221 tatgtccgca gggacagtgg cttctccagc cagggtgtgg acacctatgt ggagatgagg
     2281 cctgtctcca cttcttcaaa tgactccttc tctgagcaag acctggacaa ggaggatgga
     2341 cggcccctgg agctccggga cctgcttcac ttctccagcc aagtagccca gggcatggcc
     2401 ttcctcgctt ccaagaattg catccaccgg gacgtggcag cgcgtaacgt gctgttgacc
     2461 aatggtcatg tggccaagat tggggacttc gggctggcta gggacatcat gaatgactcc
     2521 aactacattg tcaagggcaa tgcccgcctg cctgtgaagt ggatggcccc agagagcatc
     2581 tttgactgtg tctacacggt tcagagcgac gtctggtcct atggcatcct cctctgggag
     2641 atcttctcac ttgggctgaa tccctaccct ggcatcctgg tgaacagcaa gttctataaa
     2701 ctggtgaagg atggatacca aatggcccag cctgcatttg ccccaaagaa tatatacagc
     2761 atcatgcagg cctgctgggc cttggagccc acccacagac ccaccttcca gcagatctgc
     2821 tccttccttc aggagcaggc ccaagaggac aggagagagc gggactatac caatctgccg
     2881 agcagcagca gaagcggtgg cagcggcagc agcagcagtg agctggagga ggagagctct
     2941 agtgagcacc tgacctgctg cgagcaaggg gatatcgccc agcccttgct gcagcccaac
     3001 aactatcagt tctgcttgcc aactttcttg tacaaagttg gcattataag aaagcattgc
     3061 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     3121 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     3181 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc
     3241 ctcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg
     3301 tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt
     3361 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     3421 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     3481 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     3541 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     3601 attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     3661 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     3721 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     3781 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     3841 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     3901 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     3961 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     4021 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     4081 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     4141 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     4201 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     4261 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     4321 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     4381 gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg
     4441 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4501 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4561 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4621 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4681 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4741 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4801 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4861 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4921 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4981 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5041 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5101 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5161 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     5221 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     5281 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     5341 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     5401 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     5461 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     5521 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     5581 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     5641 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     5701 gggccc
//
