LOCUS       sequence-313988-        2224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0401
FEATURES             Location/Qualifiers
     source          1..2224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     89..107
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(111..154)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      354..411
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(435..452)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(606..1194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(686..705)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1377..1471)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1492..2151)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2066..2085
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac
       61 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata
      121 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcggtctcag gagggaggcg
      181 tgtcgtttta gtgaggtcag cttagcatga gctaagcaca tacgtcagaa accattattg
      241 cgcggctatc agctaggtca cgatcactat cagcgcggta ggtcctatat aaggttttgc
      301 tattcattga aagcagtagt gactgatttg tatatatact cgagaccctg cagtccggca
      361 aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc
      421 gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      481 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      541 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      601 gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      661 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      721 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      781 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      841 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      901 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      961 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1021 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1081 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1141 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1201 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1261 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1321 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc
     1381 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     1441 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
     1501 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     1561 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     1621 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     1681 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
     1741 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     1801 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     1861 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     1921 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     1981 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     2041 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     2101 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     2161 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     2221 atgg
//
