LOCUS       sequence-31399-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MPP8
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             297..314
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(367..385)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      381..428
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      465..845
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(552..571)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     762..783
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attttgttta ctttaagaag gagatatacc atgcatcatc atcatcatca cagcagcggc
       61 agagaaaact tgtatttcca gggcgaggat gtgttcgagg tggagaagat cctggacatg
      121 aagaccgagg ggggtaaagt tctttacaaa gttcgctgga aaggctatac atcggatgat
      181 gatacctggg agcccgagat tcacctggag gactgtaaag aagtgcttct tgaatttagg
      241 aagaaaattg cagagaacaa agccaaatga tgacgaagct tgcggccgca ctcgagcacc
      301 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
      361 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
      421 gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg
      481 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      541 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      601 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
      661 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
      721 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
      781 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
      841 ttcggcctat tggttaaaaa atgagctgat ttaacaaaat ttaacgcg
//
