LOCUS       sequence-314014-        7092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CHAMP1 (CAMP) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Itoh G, Kanno S, Uchida KS, Chiba S, Sugino S, Watanabe K, Mizuno K,
            Yasui A, Hirota T, Tanaka K
  TITLE     CAMP (C13orf8, ZNF828) is a novel regulator of 
            kinetochore-microtubule attachment.
  JOURNAL   EMBO J. 2011 Jan 5;30(1):130-44. doi: 10.1038/emboj.2010.276. Epub 
            2010 Nov 9.
  PUBMED    21063390
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010.276"; journalName: "EMBO J"; date: "2011-01-5- 
            5"; volume: "30"; issue: "1"; pages: "130-44"
COMMENT     Sequence Label: pEGFP-CHAMP1
FEATURES             Location/Qualifiers
     source          1..7092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3952..4073
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3989..4008)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4043..4062
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4080..4535)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4217..4238)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4429..4448
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4562..4666
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4691..4711)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4735..4945
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4757..4953
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4804..4939
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4988..5782
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4988..5782
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5042..5061)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5652..5671
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5970..5989)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6014..6061
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6390..6978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6879..6898
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attcaatgga agcattccag
     1381 gaacttcgta aaccatcagc acgtttggag tgtgaccatt gcagtttcag aggcacagac
     1441 tatgaaaatg tacaaatcca tatgggtacc atccatccag aattttgtga tgaaatggat
     1501 gctggtgggc taggcaaaat gatattttac cagaaaagtg caaagttatt tcactgccat
     1561 aaatgcttct tcaccagcaa gatgtactct aatgtatact atcacatcac atccaaacat
     1621 gcatccccag acaaatggaa tgataaacca aaaaatcagt tgaacaaaga aacagatcct
     1681 gtgaaaagcc ctcctcttcc tgaacaccag aaaataccct gcaattcagc agaaccaaaa
     1741 tccatacctg ccctttcaat ggaaacacag aaacttggtt cagttttgtc tccagaatcg
     1801 ccaaaaccta ctcctcttac tcccctggag cctcagaaac ctggctctgt tgtttctcct
     1861 gagctacaga cacctcttcc ttctcctgag ccttcaaaac ctgcctctgt ttcttctcct
     1921 gaacctccaa aatcagtccc tgtttgtgag tctcagaaac ttgcccctgt tccttctcca
     1981 gaaccacaga aacctgcccc tgtatctcct gagtcagtaa aggctactct tagtaatccc
     2041 aaaccccaga agcagtctca tttcccggaa acattggggc caccttcagc ctcatctcca
     2101 gagtcaccag ttctagctgc ttccccagaa ccttggggac catccccagc tgcatctcca
     2161 gaatctcgga agtcagcccg gactacctcc cctgagccaa ggaagccatc cccttcagag
     2221 tctcctgaac cttggaagcc gttccctgct gtctccccag agcctaggag accagccccc
     2281 gctgtgtcac caggctcttg gaaaccaggg ccacctgggt cccctaggcc ttggaaatcc
     2341 aatccttcag catcatcagg accttggaag ccagctaaac ctgctccatc tgtgtctcct
     2401 ggaccttgga aaccaattcc ttctgtatct cctggacctt ggaaaccaac tccatctgtg
     2461 tcttctgcat cctggaaatc ttcatcagtc tcacccagct cctggaagtc tccccctgca
     2521 tctcctgagt catggaagtc tggcccacca gaactccgaa agacagctcc cacgttgtct
     2581 cctgaacatt ggaaggcagt tcccccagtg tctccagagc ttcgcaaacc cggcccacca
     2641 ctatccccag agatccgtag tccagcagga tctccagagc tcagaaaacc ctcagggtca
     2701 ccagatcttt ggaagctttc tcctgatcag cggaaaactt ctcctgcttc acttgatttc
     2761 cctgagtccc agaaaagttc ccgtggtggt tctcctgatc tctggaagtc ttcctttttt
     2821 attgagcctc agaaacctgt cttccctgag acccgaaaac caggtccttc tgggccatct
     2881 gagtccccca aagcagcctc agatatctgg aagcctgttc tctctatcga tactgagcct
     2941 agaaaacctg ccctgtttcc cgagcctgcc aaaacagccc ctcctgcttc tccagaagca
     3001 cgcaaacgtg ccctttttcc agagccccgg aagcatgccc ttttccctga actccccaaa
     3061 tctgctctat tctcagaatc acagaaggct gttgagcttg gtgatgaact acaaatagat
     3121 gccatagatg atcaaaaatg tgatattttg gttcaggaag aacttctagc ttcacctaag
     3181 aaactcttag aagatacttt atttccttcc tcaaagaagc tcaagaaaga caaccaagag
     3241 agctcagacg ctgagcttag tagtagtgag tacataaaaa cagatttgga tgcgatggat
     3301 attaagggcc aggaatcaag cagtgatcaa gagcaggttg atgtggaatc cattgatttt
     3361 agcaaagaga acaaaatgga catgactagt ccagagcagt ctagaaatgt gctacagttt
     3421 actgaagaaa aagaagcttt tatctctgaa gaggagattg caaaatacat gaagcgtgga
     3481 aaaggaaagt attattgcaa aatttgttgc tgtcgtgcta tgaaaaaagg tgctgttttg
     3541 catcatttgg ttaataagca taatgttcat agcccttaca aatgcacaat ctgtggaaag
     3601 gcttttcttt tggaatctct ccttaaaaat catgtagcag cccatgggca aagtttactt
     3661 aaatgtccac gttgtaattt tgaatcaaat ttcccaagag gttttaagaa acatttaact
     3721 cattgtcaaa gccggcataa tgaagaggca aataaaaagc taatggaagc tcttgaaccg
     3781 ccactggagg agcagcaaat tgtcgacggt accgcgggcc cgggatccac cggatctaga
     3841 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     3901 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     3961 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4021 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
     4081 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     4141 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     4201 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     4261 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     4321 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     4381 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     4441 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     4501 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     4561 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4621 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     4681 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4741 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4801 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4861 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4921 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     4981 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     5041 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5101 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5161 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5221 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5281 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5341 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5401 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5461 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     5521 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     5581 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5641 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5701 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5761 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     5821 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     5881 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     5941 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     6001 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     6061 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     6121 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     6181 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     6241 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6301 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6361 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6421 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6481 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6541 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6601 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6661 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6721 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6781 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6841 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6901 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6961 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7021 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7081 accgccatgc at
//
