LOCUS       sequence-31403-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SGF29 (3MEW)
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(524..535)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             604..621
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(674..692)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      688..735
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(859..878)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg
       61 cagagaaaac ttgtatttcc agggcaccct gcccctgtgg atcgggaagc ctggtgacaa
      121 gcccccaccc ctctgtgggg ccatccctgc ctcaggagac tacgtggcca gacctggaga
      181 caaggtggct gcccgggtga aggccgtgga tggggacgag cagtggatcc tggccgaggt
      241 ggtcagttac agccatgcca ccaacaagta tgaggtagat gacatcgatg aagaaggcaa
      301 agagagacac accctgagcc ggcgccgtgt catcccgctg ccccagtgga aggccaaccc
      361 ggagacggac cctgaggcct tgttccagaa ggagcagctc gtgctggccc tgtatcccca
      421 gactacctgc ttctaccgcg ccctgatcca tgcgccccca cagcggcccc aggatgacta
      481 ctcggtcctg tttgaagaca cctcctatgc agatggctat tcccctcccc tcaatgtggc
      541 tcagagatac gtggtggctt gtaaggaacc caagtgatga cgaagcttgc ggccgcactc
      601 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
      661 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      721 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct
      781 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
      841 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
      901 gc
//
