LOCUS       sequence-314030-        4320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Chapman S, Faulkner C, Kaiserli E, Garcia-Mata C, Savenkov EI, 
            Roberts AG, Oparka KJ, Christie JM
  TITLE     The photoreversible fluorescent protein iLOV outperforms GFP as a 
            reporter of plant virus infection.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Dec 16. 105(50):20038-43.
  PUBMED    19060199
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Dec 16. 105(50):20038-43."
COMMENT     Sequence Label: pEiLOV-N1
FEATURES             Location/Qualifiers
     source          1..4320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    1049..1170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1086..1105)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1140..1159
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1177..1632)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1314..1335)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1526..1545
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1659..1763
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1765..2122
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1765..1956
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1788..1808)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1973..2108
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2157..2951
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2157..2951
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2211..2230)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2821..2840
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3139..3158)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3183..3230
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3558..4146
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4047..4066
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttcga attctgcagt cgaccgccac catgatcgag
      601 aagaacttcg tgatcaccga ccccagactg cccgacaacc ccatcatctt cgccagcgac
      661 ggcttcctgg agctgaccga gtacagcaga gaggagatcc tgggcagaaa cgccagattc
      721 ctgcagggcc ccgagaccga ccaggccacc gtgcagaaga tcagagacgc catcagagac
      781 cagagagaga ccaccgtgca gctgatcaac tacaccaaga gcggcaagaa gttctggaac
      841 ctgctgcacc tgcagcccgt gagagaccag aagggcgagc tgcagtactt catcggcgtg
      901 cagctggacg gcagcgacca cgtgtgagcg gccgcgactc tagatcataa tcagccatac
      961 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     1021 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     1081 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1141 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
     1201 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     1261 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     1321 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     1381 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     1441 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     1501 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     1561 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     1621 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1681 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1741 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     1801 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     1861 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     1921 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     1981 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2041 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     2101 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     2161 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     2221 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     2281 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     2341 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     2401 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     2461 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     2521 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     2581 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     2641 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     2701 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     2761 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     2821 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     2881 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     2941 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     3001 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     3061 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     3121 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     3181 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     3241 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     3301 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gccagggctc
     3361 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     3421 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3481 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3541 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3601 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3661 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3721 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3781 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3841 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3901 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3961 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4021 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4081 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4141 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4201 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
     4261 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
//
