LOCUS       sequence-314033-        8635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Rao DS, Hyun TS, Kumar PD, Mizukami IF, Rubin MA, Lucas PC, Sanda 
            MG, Ross TS
  TITLE     Huntingtin-interacting protein 1 is overexpressed in prostate and 
            colon cancer and is critical for cellular survival.
  JOURNAL   J Clin Invest. 2002 Aug . 110(3):351-60.
  PUBMED    12163454
REFERENCE   2  (bases 1 to 8635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2002 Aug . 110(3):351-60."
COMMENT     Sequence Label: pcDNA3.1MycHis/HIP1
FEATURES             Location/Qualifiers
     source          1..8635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3886..3915
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3931..3948
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3992..4009)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3998..4222
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4268..4696
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4355..4374)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4565..4586
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4705..4725)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4710..5039
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4890..5025
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4952..4971
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5106..5900
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5160..5179)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5770..5789
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6074..6195
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6111..6130)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6165..6184
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6244..6260)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6244..6260)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6257..6279)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6268..6284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6292..6298,6299..6316,6317..6322))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6337..6358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6475..6492)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6646..7234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6726..6745)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7405..8196,8197..8265))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8028..8047
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8266..8370)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8445..8464)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacgggccc tctagactcg agcggccgcc actgtgctgg atccccgggg
      721 cagccgaggg cccctgactc ggctcctcgc ggcgacatgg atcggatggc cagctccatg
      781 aagcaggtgc ccaacccact gcccaaggtg ctgagccggc gcggggtcgg cgctgggctg
      841 gaggcggcgg agcgcgagag cttcgagcgg actcagactg tcagcatcaa taaggccatt
      901 aatacgcagg aagtggctgt aaaggaaaaa cacgccagaa cgtgcatact gggcacccac
      961 catgagaaag gggcacagac cttctggtct gttgtcaacc gcctgcctct gtctagcaac
     1021 gcagtgctct gctggaagtt ctgccatgtg ttccacaaac tcctccgaga tggacacccg
     1081 aacgtcctga aggactctct gagatacaga aatgaattga gtgacatgag caggatgtgg
     1141 ggccacctga gtgaggggta tggccagctg tgcagcatct acctgaaact gctaagaacc
     1201 aagatggagt accacaccaa aaatcccagg ttcccaggca acctgcagat gagtgaccgc
     1261 cagctggacg aggctggaga aagtgacgtg aacaactttt tccagttaac agtggagatg
     1321 tttgactacc tggagtgtga actcaacctc ttccaaacag tattcaactc cctggacatg
     1381 tcccgctctg tgtccgtgac ggcagcaggg cagtgccgcc tcgccccgct gatccaggtc
     1441 atcttggact gcagccacct ttatgactac actgtcaagc ttctcttcaa actccactcc
     1501 tgcctcccag ctgacaccct gcaaggccac cgggaccgct tcatggagca gtttacaaag
     1561 ttgaaagatc tgttctaccg ctccagcaac ctgcagtact tcaagcggct cattcagatc
     1621 ccccagctgc ctgagaaccc acccaacttc ctgcgagcct cagccctgtc agaacatatc
     1681 agccctgtgg tggtgatccc tgcagaggcc tcatcccccg acagcgagcc agtcctagag
     1741 aaggatgacc tcatggacat ggatgcctct cagcagaatt tatttgacaa caagtttgat
     1801 gacatctttg gcagttcatt cagcagtgat cccttcaatt tcaacagtca aaatggtgtg
     1861 aacaaggatg agaaggacca cttaattgag cgactataca gagagatcag tggattgaag
     1921 gcacagctag aaaacatgaa gactgagagc cagcgggttg tgctgcagct gaagggccac
     1981 gtcagcgagc tggaagcaga tctggccgag cagcagcacc tgcggcagca ggcggccgac
     2041 gactgtgaat tcctgcgggc agaactggac gagctcagga ggcagcggga ggacaccgag
     2101 aaggctcagc ggagcctgtc tgagatagaa aggaaagctc aagccaatga acagcgatat
     2161 agcaagctaa aggagaagta cagcgagctg gttcagaacc acgctgacct gctgcggaag
     2221 aatgcagagg tgaccaaaca ggtgtccatg gccagacaag cccaggtaga tttggaacga
     2281 gagaaaaaag agctggagga ttcgttggag cgcatcagtg accagggcca gcggaagact
     2341 caagaacagc tggaagttct agagagcttg aagcaggaac ttgccacaag ccaacgggag
     2401 cttcaggttc tgcaaggcag cctggaaact tctgcccagt cagaagcaaa ctgggcagcc
     2461 gagttcgccg agctagagaa ggagcgggac agcctggtga gtggcgcagc tcatagggag
     2521 gaggaattat ctgctcttcg gaaagaactg caggacactc agctcaaact ggccagcaca
     2581 gaggaatcta tgtgccagct tgccaaagac caacgaaaaa tgcttctggt ggggtccagg
     2641 aaggctgcgg agcaggtgat acaagacgcc ctgaaccagc ttgaagaacc tcctctcatc
     2701 agctgcgctg ggtctgcaga tcacctcctc tccacggtca catccatttc cagctgcatc
     2761 gagcaactgg agaaaagctg gagccagtat ctggcctgcc cagaagacat cagtggactt
     2821 ctccattcca taaccctgct ggcccacttg accagcgacg ccattgctca tggtgccacc
     2881 acctgcctca gagccccacc tgagcctgcc gactcactga ccgaggcctg taagcagtat
     2941 ggcagggaaa ccctcgccta cctggcctcc ctggaggaag agggaagcct tgagaatgcc
     3001 gacagcacag ccatgaggaa ctgcctgagc aagatcaagg ccatcggcga ggagctcctg
     3061 cccaggggac tggacatcaa gcaggaggag ctgggggacc tggtggacaa ggagatggcg
     3121 gccacttcag ctgctattga aactgccacg gccagaatag aggagatgct cagcaaatcc
     3181 cgagcaggag acacaggagt caaattggag gtgaatgaaa ggatccttgg ttgctgtacc
     3241 agcctcatgc aagctattca ggtgctcatc gtggcctcta aggacctcca gagagagatt
     3301 gtggagagcg gcaggggtac agcatcccct aaagagtttt atgccaagaa ctctcgatgg
     3361 acagaaggac ttatctcagc ctccaaggct gtgggctggg gagccactgt catggtggat
     3421 gcagctgatc tggtggtaca aggcagaggg aaatttgagg agctaatggt gtgttctcat
     3481 gaaattgctg ctagcacagc ccagcttgtg gctgcatcca aggtgaaagc tgataaggac
     3541 agccccaacc tagcccagct gcagcaggcc tctcggggag tgaaccaggc cactgccggc
     3601 gttgtggcct caaccatttc cggcaaatca cagatcgaag agacagacaa catggacttc
     3661 tcaagcatga cgctgacaca gatcaaacgc caagagatgg attctcaggt tagggtgcta
     3721 gagctagaaa atgaattgca gaaggagcgt caaaaactgg gagagcttcg gaaaaagcac
     3781 tacgagcttg ctggtgttgc tgagggctgg gaagaaggaa cagaggcatc tccacctaca
     3841 ctgcaagaag tggtaaccga aaaagaatgg taccaagctt acgtagaaca aaaactcatc
     3901 tcagaagagg atctgaatag cgccgtcgac catcatcatc atcatcattg agtttaaacg
     3961 gtctccagct taagtttaaa ccgctgatca gcctcgactg tgccttctag ttgccagcca
     4021 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4081 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4141 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4201 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     4261 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4321 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4381 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4441 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4501 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4561 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4621 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4681 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4741 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4801 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4861 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4921 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     4981 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     5041 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     5101 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     5161 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     5221 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     5281 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     5341 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     5401 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     5461 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     5521 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5581 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     5641 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5701 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5761 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5821 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5881 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     5941 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     6001 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     6061 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6121 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6181 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     6241 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6301 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6361 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6421 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6481 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6541 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6601 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6661 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6721 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6781 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6841 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6901 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6961 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7021 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7081 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7141 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7201 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7261 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7321 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7381 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7441 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7501 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7561 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7621 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7681 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7741 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7801 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7861 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7921 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7981 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8041 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8101 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8161 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8221 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8281 acattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8341 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8401 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     8461 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     8521 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     8581 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtt
//
