LOCUS       sequence-314034-        4651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Young JA, Sermwittayawong D, Kim HJ, Nandu S, An N, 
            Erdjument-Bromage H, Tempst P, Coscoy L, Winoto A
  TITLE     Fas-associated death domain (FADD) and the E3 ubiquitin-protein 
            ligase TRIM21 interact to negatively regulate virus-induced 
            interferon production.
  JOURNAL   J Biol Chem. 2011 Feb 25;286(8):6521-31. Epub 2010 Dec 23.
  PUBMED    21183682
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-02-25"; volume: "286(8)"; pages: "6521-31. Epub 
            2010 Dec 23"
COMMENT     Sequence Label: pCI-hFADD-FLAG
FEATURES             Location/Qualifiers
     source          1..4651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          644..776
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     759..778
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        821..839
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1486..1509
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    1552..1673
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1563..1582)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1617..1636
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      1854..2309
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1941..1960)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2151..2172
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2376..2395)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2495..2517
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2555..2573)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2641..2745
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2746..2814,2815..3606)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2964..2983)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3777..4365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4266..4285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        join(4576..4651,1..304)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatca ctagaagctt
      541 tattgcggta gtttatcaca gttaaattgc taacgcagtc agtgcttctg acacaacagt
      601 ctcgaactta agctgcagaa gttggtcgtg aggcactggg caggtaagta tcaaggttac
      661 aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg
      721 tttctgatag gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt
      781 ccactcccag ttcaattaca gctcttaagg ctagagtact taatacgact cactataggc
      841 tagcctcgag aattccccgc catggacccg ttcctggtgc tgctgcactc ggtgtcgtcc
      901 agcctgtcga gcagcgagct gaccgagctc aagttcctat gcctcgggcg cgtgggcaag
      961 cgcaagctgg agcgcgtgca gagcggccta gacctcttct ccatgctgct ggagcagaac
     1021 gacctggagc ccgggcacac cgagctcctg cgcgagctgc tcgcctccct gcggcgccac
     1081 gacctgctgc ggcgcgtcga cgacttcgag gcgggggcgg cggccggggc cgcgcctggg
     1141 gaagaagacc tgtgtgcagc atttaacgtc atatgtgata atgtggggaa agattggaga
     1201 aggctggctc gtcagctcaa agtctcagac accaagatcg acagcatcga ggacagatac
     1261 ccccgcaacc tgacagagcg tgtgcgggag tcactgagaa tctggaagaa cacagagaag
     1321 gagaacgcaa cagtggccca cctggtgggg gctctcaggt cctgccagat gaacctggtg
     1381 gctgacctgg tacaagaggt tcagcaggcc cgtgacctcc agaacaggag tggggccatg
     1441 tccccgatgt catggaactc agacgcatct acctccgaag cgtccgacta caaagacgat
     1501 gacgacaagt gatctagagt cgacccgggc ggccgcttcg agcagacatg ataagataca
     1561 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     1621 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1681 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca
     1741 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gggctggcgt aatagcgaag
     1801 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc
     1861 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     1921 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     1981 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2041 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     2101 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2161 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     2221 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2281 ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg
     2341 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     2401 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2461 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2521 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     2581 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     2641 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2701 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     2761 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     2821 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     2881 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     2941 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     3001 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3061 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3121 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     3181 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3241 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     3301 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     3361 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     3421 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     3481 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     3541 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     3601 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     3661 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3721 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3781 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3841 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3901 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3961 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4021 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4081 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4141 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4201 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4261 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4321 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4381 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatggctcg acagatcttc
     4441 aatattggcc attagccata ttattcattg gttatatagc ataaatcaat attggctatt
     4501 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     4561 tatgaccgcc atgttggcat tgattattga ctagttatta atagtaatca attacggggt
     4621 cattagttca tagcccatat atggagttcc g
//
