LOCUS       sequence-314036-        8205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Nicholls SB, Chu J, Abbruzzese G, Tremblay KD, Hardy JA
  TITLE     Mechanism of a genetically encoded dark-to-bright reporter for 
            caspase activity.
  JOURNAL   J Biol Chem. 2011 Jul 15;286(28):24977-86. Epub 2011 May 10.
  PUBMED    21558267
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-07-15"; volume: "286(28)"; pages: "24977-86. 
            Epub 2011 May 10"
COMMENT     Sequence Label: pT-CA-GFP-IRES-mLumin
FEATURES             Location/Qualifiers
     source          1..8205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     543..563
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     589..613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          753..885
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     868..887
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        930..948
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      964..973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(970..972,973..975,976..1686)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(1015..1036)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1276..1295)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1623..1644
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(1854..2428,2429..2431,2432..2440)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2021..2038)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2248..2267
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      2461..2470
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2467..3165
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3212..3333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3223..3242)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3277..3296
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3519..3974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3606..3625)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3816..3837
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4091..4448
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4091..4282
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4114..4134)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4299..4434
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4499..5293
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4553..4572)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5163..5182
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5357..5405
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5446..5465)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     repeat_region   5740..5802
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     5808..5830
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5868..5886)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5954..6058
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6059..6127,6128..6919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6277..6296)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7090..7678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7579..7598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   7756..7790
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctgcgatcgc ccgccccgtt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      601 gaaccgtcag atcactagaa gctttattgc ggtagtttat cacagttaaa ttgctaacgc
      661 agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agtgactctc ttaaggtagc
      721 cttgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca agacaggttt
      781 aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg
      841 cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc cactcccagt
      901 tcaattacag ctcttaaggc tagagtactt aatacgactc actataggct agcgctaccg
      961 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1021 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1081 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1141 tggcccaccc tcgtgaccac cttcacctac ggcgtgcagt gcttcagccg ctaccccgac
     1201 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1261 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1321 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1381 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1441 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     1501 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1561 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     1621 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctcacggcat ggacgagctg
     1681 tacaaggatg aggtcgattt tcaggggcca tgcaatgatt cgtcagaccc tctggtcgtg
     1741 gctgcctcca ttattggtat tctgcacctg atcctgtgga ttctggatcg tctgtaagaa
     1801 ttcacgcgtc gagcatgcat ctagggcggc caattccgcc cctctccccc ccccccctct
     1861 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     1921 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     1981 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     2041 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     2101 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     2161 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     2221 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     2281 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     2341 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     2401 gttttccttt gaaaaacacg atgataagct tgccacaacc cgggatcctc tagagtcgac
     2461 gccaccatgg tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc
     2521 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     2581 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     2641 ctggctacca gcttcatgta cggcagcaaa accttcatca accacaccca gggcatcccc
     2701 gacttcttta agcagtcctt ccccgagggc ttcacatggg agagagtcac cacatacgaa
     2761 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     2821 aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
     2881 ctcggctggg aggcctccac cgagaccctg taccccgctg acggcggcct ggaaggcaga
     2941 gccgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt gaagaccaca
     3001 tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggacaga
     3061 agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgaggtggct
     3121 gtggccagat actgcgacct ccctagcaaa ctggggcaca gataagcggc cgcttccctt
     3181 tagtgagggt taatgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
     3241 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     3301 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     3361 tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     3421 ggtaaaatcc gataaggatc gatccgggct ggcgtaatag cgaagaggcc cgcaccgatc
     3481 gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     3541 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     3601 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3661 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3721 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3781 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3841 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     3901 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     3961 aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4021 cgcatacgcg gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     4081 taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     4141 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4201 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4261 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4321 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4381 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4441 tttgcaaaaa gcttgattct tctgacacaa cagtctcgaa cttaaggcta gagccaccat
     4501 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     4561 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     4621 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     4681 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     4741 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4801 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4861 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     4921 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     4981 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     5041 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     5101 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     5161 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     5221 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     5281 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     5341 ccatcacgat ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     5401 gtgtgaatcg atagcgataa ggatccgcgt atggtgcact ctcagtacaa tctgctctga
     5461 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     5521 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga ttttgttact
     5581 ttatagaaga aattttgagt ttttgttttt ttttaataaa taaataaaca taaataaatt
     5641 gtttgttgaa tttattatta gtatgtaagt gtaaatataa taaaacttaa tatctattca
     5701 aattaataaa taaacctcga tatacagacc gataaaacac atgcgtcaat tttacgcatg
     5761 attatcttta acgtacgtca caatatgatt atctttctag ggttaatccg ggagctgcat
     5821 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     5881 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5941 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6001 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6061 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6121 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6181 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6241 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6301 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6361 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6421 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6481 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6541 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6601 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6661 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6721 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6781 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6841 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6901 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6961 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7021 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7081 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7141 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7201 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7261 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7321 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7381 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7441 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7501 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7561 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7621 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7681 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatggc
     7741 tcgacagatc tttaacccta gaaagatagt ctgcgtaaaa ttgacgcatg cattcttgaa
     7801 atattgctct ctctttctaa atagcgcgaa tccgtcgctg tgcatttagg acatctcagt
     7861 cgccgcttgg agctcccgtg aggcgtgctt gtcaatgcgg taagtgtcac tgattttgaa
     7921 ctataacgac cgcgtgagtc aaaatgacgc atgattatct tttacgtgac ttttaagatt
     7981 taactcatac gataattata ttgttatttc atgttctact tacgtgataa cttattatat
     8041 atatattttc ttgttataga taagatcttc aatattggcc attagccata ttattcattg
     8101 gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata
     8161 atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgtt
//
