LOCUS       sequence-31404-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FLJ22313
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             345..362
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(415..433)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      429..476
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      513..893
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(600..619)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     810..831
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attttgttta ctttaagaag gagatatacc atgcatcatc atcatcatca cagcagcggc
       61 agagaaaact tgtatttcca gggccctgtg accctcatca ttaaagcacc gaatcagaaa
      121 tacagtgacc agactattag ctgcttcttg aactggaccg tggggaaact aaaaacgcat
      181 ctatctaacg tttaccctag caaaccattg acgaaggatc agagattggt gtattcgggc
      241 agactgcttc ccgatcatct gcagctgaaa gacattctca gaaaacaaga tgagtatcat
      301 atggttcatc tagtatgatg acgaagcttg cggccgcact cgagcaccac caccaccacc
      361 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      421 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      481 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      541 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      601 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      661 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
      721 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
      781 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
      841 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggccta
//
