LOCUS       sequence-314044-        8527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8527)
  AUTHORS   Quayle SN, Chheda MG, Shukla SA, Wiedemeyer R, Tamayo P, Dewan RW, 
            Zhuang L, Huang-Hobbs E, Haidar S, Xiao Y, Ligon KL, Hahn WC, Chin L
  TITLE     Integrative functional genomics identifies RINT1 as a novel GBM 
            oncogene.
  JOURNAL   Neuro Oncol. 2012 Nov;14(11):1325-31. doi: 10.1093/neuonc/nos246. 
            Epub 2012 Oct 16.
  PUBMED    23074196
REFERENCE   2  (bases 1 to 8527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/neuonc/nos246"; journalName: "Neuro Oncol"; date: 
            "2012-11"; volume: "14"; issue: "11"; pages: "1325-31"
COMMENT     Sequence Label: pLenti6.3/V5-DEST-GFP
FEATURES             Location/Qualifiers
     source          1..8527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    625..649
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(649..651,652..654,655..1365)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(649..651,652..654,655..1365)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(694..715)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(955..974)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1302..1323
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1371..1427
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(1445..1469)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1522..1563
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1587..2175
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1640..1660)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2058..2069)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2190..2506
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2357..2492
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2554..2601
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2620..3018
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3105..3338
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3410..3531
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3447..3466)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3501..3520
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3571..3706
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3726..3745)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3727..3745)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3755..3772)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3755..3771)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3764..3786)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3913..4368
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4000..4019)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4210..4231
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4394..4498
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4499..4567,4568..5359)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4717..4736)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5530..6118
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6019..6038
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6272..6289
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6406..6427
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6442..6447,6448..6465,6466..6472)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6480..6496
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6485..6507
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6504..6520
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6504..6520
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6539..6559
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6541..6559
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6587..6813
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6814..6994
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7041..7166
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7659..7892
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8077..8121
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8388..8505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagaggatcc actagtccag
      601 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagttggcat ggtgagcaag
      661 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      721 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      781 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      841 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      901 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      961 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1021 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1081 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1141 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1201 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1261 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1321 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtactc agatctcgag
     1381 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat
     1441 aactgaccaa ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgagt
     1501 ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
     1561 acgcgtaccg gttagtaatg atcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     1621 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     1681 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     1741 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     1801 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     1861 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     1921 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     1981 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     2041 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     2101 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     2161 gggccgcctc cccgcctggc gatggtaccg gtgtggaaag tccccaggct ccccagcagg
     2221 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     2281 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     2341 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     2401 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     2461 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     2521 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     2581 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagcc tttgtctcaa
     2641 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa
     2701 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat
     2761 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct
     2821 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg
     2881 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata
     2941 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt
     3001 tatgtgtggg agggctaagc acaattcgag ctcggtacct ttaagaccaa tgacttacaa
     3061 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     3121 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     3181 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     3241 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     3301 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     3361 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     3421 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3481 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     3541 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3601 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     3661 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     3721 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     3781 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     3841 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     3901 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3961 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4021 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4081 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4141 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4201 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4261 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4321 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     4381 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4441 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4501 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4561 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4621 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4681 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4741 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4801 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4861 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4921 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4981 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5041 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5101 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5161 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5221 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5281 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5341 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5401 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5461 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5521 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5581 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5641 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5701 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5761 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5821 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5881 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5941 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6001 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6061 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6121 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6181 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6241 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6301 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     6361 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     6421 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     6481 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     6541 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     6601 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     6661 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     6721 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     6781 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     6841 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6901 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6961 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     7021 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     7081 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     7141 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     7201 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     7261 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     7321 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     7381 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     7441 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     7501 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     7561 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     7621 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     7681 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     7741 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     7801 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     7861 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     7921 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     7981 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     8041 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     8101 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     8161 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     8221 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     8281 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     8341 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     8401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     8461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga taagcttggg
     8521 agttccg
//
