LOCUS       sequence-314048-        4599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Hornung V, Ablasser A, Charrel-Dennis M, Bauernfeind F, Horvath G, 
            Caffrey DR, Latz E, Fitzgerald KA
  TITLE     AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating 
            inflammasome with ASC.
  JOURNAL   Nature. 2009 Mar 26. 458(7237):514-8.
  PUBMED    19158675
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Mar 26. 458(7237):514-8."
COMMENT     Sequence Label: pCI-ASC-HA
FEATURES             Location/Qualifiers
     source          1..4599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          720..852
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     835..854
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        897..915
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             941..967
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1576..1697
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1587..1606)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1641..1660
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      1878..2333
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1965..1984)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2175..2196
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2400..2419)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2519..2541
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2579..2597)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2665..2769
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2770..2838,2839..3630)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2988..3007)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3801..4389
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4290..4309
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcactag aagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg
      661 cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg
      721 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
      781 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
      841 ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat
      901 acgactcact ataggctagc ctcgagaatt cgaaatggcc tacccatacg acgtgccaga
      961 ctacgcaggg cgcgcgcgcg acgccatcct ggatgcgctg gagaacctga ccgccgagga
     1021 gctcaagaag ttcaagctga agctgctgtc ggtgccgctg cgcgagggct acgggcgcat
     1081 cccgcggggc gcgctgctgt ccatggacgc cttggacctc accgacaagc tggtcagctt
     1141 ctacctggag acctacggcg ccgagctcac cgctaacgtg ctgcgcgaca tgggcctgca
     1201 ggagatggcc gggcagctgc aggcggccac gcaccagggc tctggagccg cgccagctgg
     1261 gatccaggcc cctcctcagt cggcagccaa gccaggcctg cactttatag accagcaccg
     1321 ggctgcgctt atcgcgaggg tcacaaacgt tgagtggctg ctggatgctc tgtacgggaa
     1381 ggtcctgacg gatgagcagt accaggcagt gcgggccgag cccaccaacc caagcaagat
     1441 gcggaagctc ttcagtttca caccagcctg gaactggacc tgcaaggact tgctcctcca
     1501 ggccctaagg gagtcccagt cctacctggt ggaggacctg gagcggagct aggcggccgc
     1561 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     1621 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     1681 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     1741 agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg
     1801 atccgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     1861 gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     1921 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     1981 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     2041 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     2101 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     2161 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     2221 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     2281 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg
     2341 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     2401 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     2461 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     2521 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     2581 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     2641 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     2701 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     2761 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     2821 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     2881 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     2941 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     3001 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     3061 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     3121 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     3181 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     3241 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     3301 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     3361 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     3421 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     3481 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     3541 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     3601 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     3661 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     3721 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     3781 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3841 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3901 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3961 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4021 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4081 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4141 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4201 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4261 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4321 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4381 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4441 gctcacatgg ctcgacagat cttcaatatt ggccattagc catattattc attggttata
     4501 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
     4561 acatttatat tggctcatgt ccaatatgac cgccatgtt
//
