LOCUS       sequence-31405-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EPHA1 (3HIL)
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             321..338
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(391..409)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      405..452
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      489..869
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(576..595)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     786..807
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaattccctc tgaatatttt gtttacttta agaaggagat ataccatgca tcatcatcat
       61 catcacagca gcggcagaga aaacttgtat ttccagggcg atgggatccc gtatcgaacc
      121 gtctctgagt ggctcgagtc catacgcatg aaacgctaca tcctgcactt ccactcggct
      181 gggctggaca ccatggagtg tgtgctggag ctgaccgctg aggacctgac gcagatggga
      241 atcacactgc ccgggcacca gaagcgcatt ctttgcagta ttcagggatt ctgatgacga
      301 agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag
      361 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      421 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg
      481 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      541 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      601 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
      661 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
      721 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
      781 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
      841 tgatttataa gggattttgc cgatttcggc ctattggtta aaaatgagct gatttaacaa
      901 aaatttaacg cg
//
