LOCUS       sequence-31406-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EPHA1 (3KKA)
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             319..336
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(389..407)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      403..450
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      487..867
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(574..593)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     784..805
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca
       61 tcacagcagc ggcagagaaa acttgtattt ccagggcgat gggatcccgt atcgaaccgt
      121 ctctgagtgg ctcgagtcca tacgcatgaa acgctacatc ctgcacttcc actcggctgg
      181 gctggacacc atggagtgtg tgctggagct gaccgctgag gacctgacgc agatgggaat
      241 cacactgccc gggcaccaga agcgcattct ttgcagtatt cagggattct gatgacgaag
      301 cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
      361 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      421 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
      481 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      541 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      601 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      661 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      721 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      781 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      841 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      901 aatttaacgc g
//
