LOCUS       sequence-31407-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EPHA2 (3KKA)
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             327..344
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(397..415)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      411..458
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(582..601)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     792..813
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attcctctga atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat
       61 cacagcagcg gcagagaaaa cttgtatttc cagggcgtgc ccttccgcac ggtgtccgag
      121 tggctggagt ccatcaagat gcagcagtat acggagcact tcatggcggc cggctacact
      181 gccatcgaga aggtggtgca gatgaccaac gacgacatca agaggattgg ggtgcggctg
      241 cccggccacc agaagcgcat cgcctacagc ctgctgggac tcaaggacca ggtgaactga
      301 tgacgaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
      361 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      421 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      481 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      541 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      601 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
      661 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
      721 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
      781 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
      841 ttc
//
