LOCUS       sequence-31408-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EPHA7 (3H8M)
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             342..359
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(412..430)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      426..473
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(597..616)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     807..828
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttccctctga atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat
       61 cacagcagcg gcagagaaaa cttgtatttc cagggcacac cagactttac cacgttctgc
      121 tcggtcggtg agtggttaca agctataaaa atggaacggt ataaggataa ctttaccgcc
      181 gcaggataca atagcctgga atctgttgcg cgtatgacta tcgaggacgt gatgagtttg
      241 ggcattacac ttgtaggtca tcagaaaaaa attatgtcat ccatccagac gatgcgcgcc
      301 caaatgctgc actgatgacg aagcttgcgg ccgcactcga gcaccaccac caccaccact
      361 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      421 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      481 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      541 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      601 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      661 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
      721 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
      781 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
      841 ccctatctcg gtctattctt ttga
//
