LOCUS       sequence-314085-        7126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Integrin/Focal Adhesions, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7126)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Integrin-Beta1-N-18
FEATURES             Location/Qualifiers
     source          1..7126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3007..3016
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3013..3015,3016..3018,3019..3732)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3058..3079)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3319..3338)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3666..3687
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3854..3975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3891..3910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3945..3964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3982..4437)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4119..4140)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4331..4350
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4464..4568
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4570..4927
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4570..4761
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4593..4613)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4778..4913
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4962..5756
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4962..5756
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5016..5035)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5626..5645
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5944..5963)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5988..6035
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6364..6952
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6853..6872
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagac cgccatgaat ttacaaccaa ttttctggat tggactgatc
      601 agttcagttt gctgtgtgtt tgctcaaaca gatgaaaata gatgtttaaa agcaaatgcc
      661 aaatcatgtg gagaatgtat acaagcaggg ccaaattgtg ggtggtgcac aaattcaaca
      721 tttttacagg aaggaatgcc tacttctgca cgatgtgatg atttagaagc cttaaaaaag
      781 aagggttgcc ctccagatga catagaaaat cccagaggct ccaaagatat aaagaaaaat
      841 aaaaatgtaa ccaaccgtag caaaggaaca gcagagaagc tcaagccaga ggatattact
      901 cagatccaac cacagcagtt ggttttgcga ttaagatcag gggagccaca gacatttaca
      961 ttaaaattca agagagctga agactatccc attgacctct actaccttat ggacctgtct
     1021 tactcaatga aagacgattt ggagaatgta aaaagtcttg gaacagatct gatgaatgaa
     1081 atgaggagga ttacttcgga cttcagaatt ggatttggct catttgtgga aaagactgtg
     1141 atgccttaca ttagcacaac accagctaag ctcaggaacc cttgcacaag tgaacagaac
     1201 tgcaccagcc catttagcta caaaaatgtg ctcagtctta ctaataaagg agaagtattt
     1261 aatgaacttg ttggaaaaca gcgcatatct ggaaatttgg attctccaga aggtggtttc
     1321 gatgccatca tgcaagttgc agtttgtgga tcactgattg gctggaggaa tgttacacgg
     1381 ctgctggtgt tttccacaga tgccgggttt cactttgctg gagatgggaa acttggtggc
     1441 attgttttac caaatgatgg acaatgtcac ctggaaaata atatgtacac aatgagccat
     1501 tattatgatt atccttctat tgctcacctt gtccagaaac tgagtgaaaa taatattcag
     1561 acaatttttg cagttactga agaatttcag cctgtttaca aggagctgaa aaacttgatc
     1621 cctaagtcag cagtaggaac attatctgca aattctagca atgtaattca gttgatcatt
     1681 gatgcataca attccctttc ctcagaagtc attttggaaa acggcaaatt gtcagaagga
     1741 gtaacaataa gttacaaatc ttactgcaag aacggggtga atggaacagg ggaaaatgga
     1801 agaaaatgtt ccaatatttc cattggagat gaggttcaat ttgaaattag cataacttca
     1861 aataagtgtc caaaaaagga ttctgacagc tttaaaatta ggcctctggg ctttacggag
     1921 gaagtagagg ttattcttca gtacatctgt gaatgtgaat gccaaagcga aggcatccct
     1981 gaaagtccca agtgtcatga aggaaatggg acatttgagt gtggcgcgtg caggtgcaat
     2041 gaagggcgtg ttggtagaca ttgtgaatgc agcacagatg aagttaacag tgaagacatg
     2101 gatgcttact gcaggaaaga aaacagttca gaaatctgca gtaacaatgg agagtgcgtc
     2161 tgcggacagt gtgtttgtag gaagagggat aatacaaatg aaatttattc tggcaaattc
     2221 tgcgagtgtg ataatttcaa ctgtgataga tccaatggct taatttgtgg aggaaatggt
     2281 gtttgcaagt gtcgtgtgtg tgagtgcaac cccaactaca ctggcagtgc atgtgactgt
     2341 tctttggata ctagtacttg tgaagccagc aacggacaga tctgcaatgg ccggggcatc
     2401 tgcgagtgtg gtgtctgtaa gtgtacagat ccgaagtttc aagggcaaac gtgtgagatg
     2461 tgtcagacct gccttggtgt ctgtgctgag cataaagaat gtgttcagtg cagagccttc
     2521 aataaaggag aaaagaaaga cacatgcaca caggaatgtt cctattttaa cattaccaag
     2581 gtagaaagtc gggacaaatt accccagccg gtccaacctg atcctgtgtc ccattgtaag
     2641 gagaaggatg ttgacgactg ttggttctat tttacgtatt cagtgaatgg gaacaacgag
     2701 gtcatggttc atgttgtgga gaatccagag tgtcccactg gtccagacat cattccaatt
     2761 gtagctggtg tggttgctgg aattgttctt attggccttg cattactgct gatatggaag
     2821 cttttaatga taattcatga cagaagggag tttgctaaat ttgaaaagga gaaaatgaat
     2881 gccaaatggg acacgggtga aaatcctatt tataagagtg ccgtaacaac tgtggtcaat
     2941 ccgaagtatg agggaaaagg tagcgcatcc gggggtgcgt caggcggcag cggatctgga
     3001 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3061 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3121 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3181 ccctggccca ccctcgtgac caccttgacc tacggcgtgc agtgcttcgc ccgctacccc
     3241 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3301 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3361 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3421 atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat caccgccgac
     3481 aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga ggacggcagc
     3541 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     3601 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     3661 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     3721 ctgtacaagt aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     3781 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     3841 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3901 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3961 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     4021 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     4081 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     4141 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     4201 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     4261 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     4321 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     4381 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     4441 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4501 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4561 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     4621 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4681 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4741 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4801 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4861 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     4921 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     4981 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     5041 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     5101 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     5161 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     5221 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     5281 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     5341 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     5401 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     5461 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     5521 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     5581 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     5641 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     5701 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     5761 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     5821 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     5881 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     5941 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     6001 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     6061 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     6121 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     6181 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     6241 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6301 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6361 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6421 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6481 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6541 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6601 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6661 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6721 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6781 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6841 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6901 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6961 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7021 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt
     7081 aatcaattac ggggtcatta gttcatagcc catatatgga gttccg
//
