LOCUS       sequence-31409-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CBX5 (3I3C)
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             326..343
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(396..414)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      410..457
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      494..874
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(581..600)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     791..812
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttccctctga atattttgtt tactttagaa ggagatatac catgcatcat catcatcatc
       61 acagcagcgg cagagaaaac ttgtatttcc agggcagaga gcagagcaat gatatcgctc
      121 ggggctttga gagaggactg gaaccagaaa agatcattgg ggcaacagat tcctgtggtg
      181 atttaatgtt cctaatgaaa tggaaagaca cagatgaagc tgacctggtt cttgcaaaag
      241 aagctaatgt gaaatgtcca caaattgtga tagcatttta tgaagagaga ctgacatgat
      301 gacgaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      361 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      421 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      481 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      541 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
      601 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
      661 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
      721 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
      781 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
      841 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
      901 taac
//
