LOCUS       sequence-314093-       10140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SH2 membrane localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Carrasco-Lopez C, Zhao EM, Gil AA, Alam N, Toettcher JE, Avalos JL
  TITLE     Development of light-responsive protein binding in the monobody 
            non-immunoglobulin scaffold.
  JOURNAL   Nat Commun. 2020 Aug 13;11(1):4045. doi: 10.1038/s41467-020-17837-7.
  PUBMED    32792484
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-08-13"; volume: "11(1):4045. doi"; pages: " 
            10.1038/s41467-020-17837-7"
COMMENT     Sequence Label: pHR_SH2-mCherry-CAAX
FEATURES             Location/Qualifiers
     source          1..10140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             813..1517
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(955..973)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1223..1242
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1685..2273
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1738..1758)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2156..2167)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2276..2292)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2277..2293)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2383..2616
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2641..2659)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2642..2659)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2949..2968)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3182..3201)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3301..3323
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3361..3379)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3447..3551
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3552..3620,3621..4412)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3770..3789)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4583..5171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5072..5091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5325..5342
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5412..5432)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5417..5746
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5597..5732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          6880..6945
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7075..7095
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7520..7654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7557..7576)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7611..7630
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7822..8455
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8502..8627
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9124..9357
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9542..9586
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9871..9988
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag
      421 ctctcgagaa ttctcacgcg tcaagtggag caaggcaggt ggacagtgga tccatgtcac
      481 tggaaaagca ctcctggtat cacggtccag taagccgtaa cgcagcagaa tatcttctgt
      541 cgtcaggcat aaacggctct ttccttgtca gagagtcaga gtccagcccc ggtcagcgtt
      601 caatatcttt acgttatgag ggccgcgttt accattatcg catcaacacg gcttctgatg
      661 gaaaactgta tgtctcgtcc gaaagccgtt ttaatactct tgcggaactg gtacaccatc
      721 actccactgt cgctgacggc ctgatcacga ccctgcacta cccagcacca aagagaaata
      781 agcctacagt gtacggggtg tcgccaaatt acgtgagcaa gggcgaggag gataacatgg
      841 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
      901 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      961 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1021 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     1081 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     1141 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     1201 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     1261 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     1321 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     1381 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     1441 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     1501 tggacgagct gtacaaggga agcgggagtg ggagcaaaaa gaagaaaaag aaggcaaaga
     1561 caaagtgtgt aattatgtag cctgcaggcg gccgcaactc ccacctgcaa catgcgtgac
     1621 tgactgaggc cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat
     1681 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     1741 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     1801 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     1861 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     1921 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     1981 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     2041 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     2101 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     2161 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     2221 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     2281 ccgtcgacct cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg
     2341 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     2401 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     2461 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     2521 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     2581 tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat
     2641 tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc
     2701 gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac
     2761 cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt
     2821 ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc
     2881 tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     2941 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     3001 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
     3061 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     3121 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca
     3181 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     3241 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     3301 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     3361 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     3421 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3481 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3541 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     3601 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     3661 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     3721 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     3781 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3841 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3901 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3961 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4021 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4081 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4141 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4201 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4261 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4321 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4381 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4441 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4501 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5281 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5341 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     5401 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     5461 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     5521 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     5581 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5641 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5701 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
     5761 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     5821 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     5881 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     5941 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     6001 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     6061 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     6121 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     6181 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
     6241 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     6301 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
     6361 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     6421 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
     6481 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     6541 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     6601 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     6661 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
     6721 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
     6781 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
     6841 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     6901 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     6961 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     7021 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     7081 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     7141 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     7201 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
     7261 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
     7321 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
     7381 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
     7441 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     7501 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     7561 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7621 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     7681 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     7741 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     7801 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     7861 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     7921 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     7981 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     8041 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     8101 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     8161 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     8221 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     8281 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8341 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8401 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     8461 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     8521 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     8581 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     8641 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     8701 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     8761 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     8821 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     8881 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     8941 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     9001 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     9061 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     9121 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     9181 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     9241 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     9301 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     9361 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     9421 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     9481 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     9541 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     9601 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     9661 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     9721 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     9781 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     9841 gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     9901 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     9961 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
    10021 ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa gattttattt
    10081 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagctgca
//
