LOCUS       sequence-31410-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FXR2
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             498..515
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(568..586)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      582..629
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(753..772)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaattccctc tgaatatttt gtttacttta agaaggagat ataccatgca tcatcatcat
       61 catcacagca gcggcagaga aaacttgtat ttccagggcc tgcccgtcga ggtgcgcggc
      121 tccaacgggg ccttctacaa gggctttgtg aaggatgtcc atgaagactc tgtcaccatc
      181 ttctttgaaa acaactggca gagtgagaga caaattcctt ttggggatgt ccggctacca
      241 cctccagctg actataataa ggagatcaca gaaggggatg aagtggaggt ttattctcga
      301 gccaatgaac aggaaccttg tggctggtgg ctggcccggg tgcggatgat gaagggagat
      361 ttctatgtca ttgaatatgc tgcctgtgat gccacctaca atgaaattgt taccctggag
      421 cgacttcggc cagttaatcc caatcccctt gcaaccaaag gcagcttctg atgacgaagc
      481 ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
      541 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      601 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga
      661 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      721 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      781 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      841 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      901 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttga
//
