LOCUS       sequence-31411-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DNMT3A
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             565..582
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(635..653)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      649..696
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(820..839)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg
       61 cagagaaaac ttgtatttcc agggcggcga tgacgagcca gagtacgagg acggccgggg
      121 ctttggcatt ggggagctgg tgtgggggaa actgcggggc ttctcctggt ggccaggccg
      181 cattgtgtct tggtggatga cgggccggag ccgagcagct gaaggcaccc gctgggtcat
      241 gtggttcgga gacggcaaat tctcagtggt gtgtgttgag aagctgatgc cgctgagctc
      301 gttttgcagt gcgttccacc aggccacgta caacaagcag cccatgtacc gcaaagccat
      361 ctacgaggtc ctgcaggtgg ccagcagccg cgcggggaag ctgttcccgg tgtgccacga
      421 cagcgatgag agtgacactg ccaaggccgt ggaggtgcag aacaagccca tgattgaatg
      481 ggccctgggg ggcttccagc cttctggccc taagggcctg gagccaccag aagaatgatg
      541 acgaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac
      601 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      661 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      721 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      781 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      841 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ct
//
