LOCUS       sequence-314145-        6891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive transcription of sacA crRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6891)
  TITLE     Cui Lab Plasmids
REFERENCE   2  (bases 1 to 6891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBsacA
FEATURES             Location/Qualifiers
     source          1..6891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             2374..3378
                     /codon_start=1
                     /gene="repB"
                     /product="RepB replication protein"
                     /label=repB
                     /note="from Enterococcus faecalis plasmid pAM-alpha-1"
                     /db_xref="GI:22652809"
                     /protein_id="AAN03827.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     terminator      5351..5422
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(6722..6741)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6841..6863
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagtagaaga agcttggaga caaggtaaag gataaaacag
     2041 cacaattcca agaaaaacac gatttagaac ctaaaaagaa cgaatttgaa ctaactcata
     2101 accgagaggt aaaaaaagaa cgaagtcgag atcagggaat gagtttataa aataaaaaaa
     2161 gcacctgaaa aggtgtcttt ttttgatggt tttgaacttg ttctttctta tcttgataca
     2221 tatagaaata acgtcatttt tattttagtt gctgaaaggt gcgttgaagt gttggtatgt
     2281 atgtgtttta aagtattgaa aacccttaaa attggttgca cagaaaaacc ccatctgtta
     2341 aagttataag tgactaaaca aataactaaa tagatggggg tttcttttaa tattatgtgt
     2401 cctaatagta gcatttattc agatgaaaaa tcaagggttt tagtggacaa gacaaaaagt
     2461 ggaaaagtga gaccatggag agaaaagaaa atcgctaatg ttgattactt tgaacttctg
     2521 catattcttg aatttaaaaa ggctgaaaga gtaaaagatt gtgctgaaat attagagtat
     2581 aaacaaaatc gtgaaacagg cgaaagaaag ttgtatcgag tgtggttttg taaatccagg
     2641 ctttgtccaa tgtgcaactg gaggagagca atgaaacatg gcattcagtc acaaaaggtt
     2701 gttgctgaag ttattaaaca aaagccaaca gttcgttggt tgtttctcac attaacagtt
     2761 aaaaatgttt atgatggcga agaattaaat aagagtttgt cagatatggc tcaaggattt
     2821 cgccgaatga tgcaatataa aaaaattaat aaaaatcttg ttggttttat gcgtgcaacg
     2881 gaagtgacaa taaataataa agataattct tataatcagc acatgcatgt attggtatgt
     2941 gtggaaccaa cttattttaa gaatacagaa aactacgtga atcaaaaaca atggattcaa
     3001 ttttggaaaa aggcaatgaa attagactat gatccaaatg taaaagttca aatgattcga
     3061 ccgaaaaata aatataaatc ggatatacaa tcggcaattg acgaaactgc aaaatatcct
     3121 gtaaaggata cggattttat gaccgatgat gaagaaaaga atttgaaacg tttgtctgat
     3181 ttggaggaag gtttacaccg taaaaggtta atctcctatg gtggtttgtt aaaagaaata
     3241 cataaaaaat taaaccttga tgacacagaa gaaggcgatt tgattcatac agatgatgac
     3301 gaaaaagccg atgaagatgg attttctatt attgcaatgt ggaattggga acggaaaaat
     3361 tattttatta aagagtagtt caacaaacgg gccagtttgt tgaagattag atgctataat
     3421 tgttattaaa aggattgaag gatgcttagg aagacgagtt attaatagct gaataagaac
     3481 ggtgctctcc aaatattctt atttagaaaa gcaaatctaa aattatctga aaagggaatg
     3541 agaatagtga atggaccaat aataatgact agagaagaaa gaatgaagat tgttcatgaa
     3601 attaaggaac gaatattgga taaatatggg gatgatgtta aggctattgg tgtttatggc
     3661 tctcttggtc gtcagactga tgggccctat tcggatattg agatgatgtg tgtcatgtca
     3721 acagaggaag cagagttcag ccatgaatgg acaaccggtg agtggaaggt ggaagtgaat
     3781 tttgatagcg aagagattct actagattat gcatctcagg tggaatcaga ttggccgctt
     3841 acacatggtc aatttttctc tattttgccg atttatgatt caggtggata cttagagaaa
     3901 gtgtatcaaa ctgctaaatc ggtagaagcc caaacgttcc acgatgcgat ttgtgccctt
     3961 atcgtagaag agctgtttga atatgcaggc aaatggcgta atattcgtgt gcaaggaccg
     4021 acaacatttc taccatcctt gactgtacag gtagcaatgg caggtgccat gttgattggt
     4081 ctgcatcatc gcatctgtta tacgacgagc gcttcggtct taactgaagc agttaagcaa
     4141 tcagatcttc cttcaggtta tgaccatctg tgccagttcg taatgtctgg tcaactttcc
     4201 gactctgaga aacttctgga atcgctagag aatttctgga atgggattca ggagtggaca
     4261 gaacgacacg gatatatagt ggatgtgtca aaacgcatac cattttgaac gatgacctct
     4321 aataattgtt aatcatgttg gttacgtatt tattaacttc tcctagtatt agtaattatc
     4381 atggctgtca tggcgcatta acggaataaa gggtgtgctt aaatcgggcc attttgcgta
     4441 ataagaaaaa ggattaatta tgagcgaatt gaattaataa taaggtaata gatttacatt
     4501 agaaaatgaa aggggatttt atgcgtgaga atgttacagt ctatcccggc attgccagtc
     4561 ggggatatta aaaagagtat aggtttttat tgcgataaac taggtttcac tttggttcac
     4621 catgaagatg gattcgcagt tctaatgtgt aatgaggttc ggattcatct atgggaggca
     4681 agtgatgaag gctggcgctc tcgtagtaat gattcaccgg tttgtacagg tgcggagtcg
     4741 tttattgctg gtactgctag ttgccgcatt gaagtagagg gaattgatga attatatcaa
     4801 catattaagc ctttgggcat tttgcacccc aatacatcat taaaagatca gtggtgggat
     4861 gaacgagact ttgcagtaat tgatcccgac aacaatttga ttagcttttt tcaacaaata
     4921 aaaagctaaa atctattatt aatctgttca gcaatcgggc gcgattgctg aataaaagat
     4981 acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta cactagaaaa
     5041 ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc tagacaaaac
     5101 ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga tcttctcaaa
     5161 aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc cccctttgct
     5221 gaggtggcag agggcaggtt tattaacgtt gatataattt aaattttatt tgacaaaaat
     5281 gggctcgtgt tgtacaataa atgtaatttc tactgttgta gattcggccg cctcgattat
     5341 aacaagattt caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     5401 ttgtttgtcg gtgaacgctc tcctttgtct gcaactgaaa agtttatact acataagtgt
     5461 ccaaattccg acattcattt cagatttccc ggtcgcactg gctgttactt catgatttcc
     5521 cgggaaagga aaatatcttg cactaaagac ctcttctccg ttattgataa agatttccac
     5581 agatgacgcg tcgagaaaca tgtgtacggt atgcaaatcc ttgatgcggc aatgtctcgc
     5641 ttctgttctt tttccgtcaa agctctttcg ttccagcgta acaatcccct cgtctttatg
     5701 gaaggaaaac gtagccgttc cgcggatact gattgaaaag ccagactccg tatgaatgtc
     5761 ttccagtaga atctcagttc tttcaggttt ttccactgga agcgcaccag atgatccatg
     5821 catgttgatg tgtatttttt tctcatttct gcgcatggct tttagctcag gcagcggctg
     5881 ctgaatcagc ttctgtcctg aaagcgtcag ctgtctcggc agcgtcatgc agtgaatcca
     5941 gtggcagtca atggtcggat gggacccttc atcctgatca ggcaccgcca tccatgcaaa
     6001 taaaatccgc cttccctgat cgtcttcaag tgtttgcggc gcgtaaaaaa atctccactc
     6061 tgtcaggtta tcagaagcaa acaacacagc ctgccctttc aaattctctg tttgcgcacc
     6121 aatcaccata taccatgtgc cttcatgctc ccataccttc ggatcgcgaa aatgcgccgt
     6181 atatccttcc ggaaggcggg cgacgacacc ctttttctca aaggacagcc cgtcgtcaga
     6241 aacagcaagg cattgatacg tttcccgatt tccatcctga tccctgacat ttcctgtgta
     6301 aaaaagatag agccgatcgt ctttcgtgac agcgctgccc gagtagcagc cgtttttatc
     6361 aaaccaatca ctcggagcca gcgcaatctc ttcccgcttc caattcacaa catcctgtgt
     6421 cgtataatgc ccccaaaatt ttgcgccgtg ccccgtctga aacggctgcc actgaaagaa
     6481 tacatgatag ctgcccttcc aataaatcac gccattcggg tcattcagca gcccaaccgg
     6541 cggcatgata tgaaaatgct ggcggtgcgg atcgctgtta gcgatgggct cttttttctc
     6601 cacttcttca taagcccggc gacgaagctc ctggtcatgt gctgtcatga attcgcctga
     6661 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     6721 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     6781 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     6841 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg a
//
