LOCUS       sequence-314157-        8170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 8170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: Kras (G12V)-pcw107
FEATURES             Location/Qualifiers
     source          1..8170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..501
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     420..438
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    522..546
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(564..596,597..599,600..1130)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(1132..1156)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1188..1776
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1241..1261)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1659..1670)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1848..2081
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2159..2280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2196..2215)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2250..2269
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2320..2455
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2475..2494)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2476..2494)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2504..2521)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2504..2520)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2513..2535)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2662..3117
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2749..2768)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2959..2980
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3143..3247
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3248..3316,3317..4108)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3466..3485)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4279..4867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4768..4787
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5021..5038
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5155..5176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5191..5196,5197..5214,5215..5221)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5229..5245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5234..5256
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5253..5269
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5253..5269
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5288..5308
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5290..5308
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5336..5562
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5563..5743
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5790..5915
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6408..6641
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6826..6870
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7101..7311
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7123..7319
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7170..7305
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7351..7953
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(7351..7370)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7847..7867
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7996..8113
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
       61 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      121 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      181 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      241 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      301 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
      361 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
      421 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
      481 atcaccgacc tctctcccca ggggctagca tcgatggatc aacaagtttg tacaaaaaag
      541 ttggcacgtg cggcggcggc accatgactg aatataaact tgtggtagtt ggagctgttg
      601 gcgtaggcaa gagtgccttg acgatacagc taattcagaa tcattttgtg gacgaatatg
      661 atccaacaat agaggattcc tacaggaagc aagtagtaat tgatggagaa acctgtctct
      721 tggatattct cgacacagca ggtcaagagg agtacagtgc aatgagggac cagtacatga
      781 ggactgggga gggctttctt tgtgtatttg ccataaataa tactaaatca tttgaagata
      841 ttcaccatta tagagaacaa attaaaagag ttaaggactc tgaagatgta cctatggtcc
      901 tagtaggaaa taaatgtgat ttgccttcca gaacagtaga cacaaaacag gctcaggact
      961 tagcaagaag ttatggaatt ccttttattg aaacatcagc aaagacaaga cagggtgttg
     1021 atgatgcctt ctatacatta gttcgagaaa ttcgaaaaca taaagaaaag atgagcaaag
     1081 atggtaaaaa gaagaaaaag aagtcaaaga caaagtgtgt aattatgtaa ttgccaactt
     1141 tcttgtacaa agtggttacc ggttagtaat gaacgcgtta agtcgacaat caacctctgg
     1201 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     1261 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     1321 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     1381 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     1441 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     1501 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     1561 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     1621 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     1681 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     1741 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     1801 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     1861 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     1921 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     1981 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     2041 atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg
     2101 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     2161 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     2221 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     2281 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     2341 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     2401 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     2461 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     2521 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     2581 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     2641 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     2701 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     2761 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     2821 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     2881 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     2941 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     3001 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     3061 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     3121 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3181 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3241 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     3301 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     3361 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     3421 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     3481 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     3541 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     3601 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     3661 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     3721 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     3781 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     3841 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     3901 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     3961 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4021 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     4081 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     4141 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4201 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4261 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4321 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4381 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     4441 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4501 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4561 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4621 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4681 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4741 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4801 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4861 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4921 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     4981 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     5041 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     5101 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     5161 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     5221 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     5281 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     5341 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     5401 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     5461 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     5521 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     5581 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5641 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5701 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     5761 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     5821 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     5881 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     5941 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     6001 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     6061 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     6121 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     6181 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     6241 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     6301 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     6361 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     6421 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     6481 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     6541 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     6601 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     6661 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     6721 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     6781 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     6841 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     6901 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     6961 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     7021 cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca accaggtgtg
     7081 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc aattagtcag
     7141 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7201 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7261 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     7321 agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc
     7381 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     7441 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     7501 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     7561 gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     7621 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     7681 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     7741 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     7801 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     7861 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     7921 tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga gacaaatggc
     7981 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     8041 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     8101 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga
     8161 ggcctgcagg
//
