LOCUS       sequence-31416-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CBX3 (3KUP)
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             309..326
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(379..397)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      393..440
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      477..857
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(564..583)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     774..795
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat cncatncagc
       61 ggcagagaaa acttgtattt ccagggcgct gctgacaaac caagaggatt tgccagaggt
      121 cttgatcctg aaagaataat tggtgccaca gacagcagtg gagaattgat gtttctcatg
      181 aaatggaaag attcagatga ggcagacttg gtgctggcga aagaggcaaa tatgaagtgt
      241 cctcaaattg taattgcttt ttatgaagag agactaactt gatgacgaag cttgcggccg
      301 cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      361 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      421 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc
      481 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      541 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      601 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      661 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      721 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
      781 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
      841 gattttgccg atttcggcct attggttaaa aaatgagctg a
//
