LOCUS       sequence-314160-        9365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9365)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 9365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: GPR110-Tango
FEATURES             Location/Qualifiers
     source          1..9365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3689..4303,4304..4690)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3756..3775)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(4722..4739)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4728..4952
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4998..5426
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5085..5104)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5295..5316
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5435..5455)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5440..5769
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5620..5755
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5682..5701
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5836..6630
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5890..5909)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6500..6519
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6804..6925
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6841..6860)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6895..6914
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6974..6990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6974..6990)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6987..7009)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6998..7014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7022..7028,7029..7046,7047..7052))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7067..7088
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7205..7222)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7376..7964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7456..7475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8135..8926,8927..8995))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8758..8777
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8996..9100)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9175..9194)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttctgcagat atccagcaca gtggcggccg cgccaccatg aagacgatca tcgccctgag
      781 ctacatcttc tgcctggtat tcgccgacta caaggacgat gatgacgcca gcatcgatat
      841 gaaagtcggc gtgctgtggc tcatatcttt ttttacattc accgacggac acggtggctt
      901 cctgggtaaa aacgacggca ttaagacaaa aaaggagctc attgtgaata agaagaagca
      961 cctggggccc gtagaggagt atcagctgct gctgcaggta acatatcgcg acagtaaaga
     1021 gaaaagggac cttcgaaact tcctcaaatt gctgaaacct cccttgctct ggtcacatgg
     1081 cctcattaga attattcgcg ccaaggccac caccgactgc aattccctta acggcgtgct
     1141 gcagtgcacc tgcgaagata gctacacttg gtttcctcca agctgtcttg atccacagaa
     1201 ctgttatctg cacacagctg gggccctccc ttcctgtgag tgccacctga acaatctgtc
     1261 acagagtgtg aatttttgtg aacggactaa aatctggggc acgtttaaga tcaatgagcg
     1321 cttcactaat gacttgctga acagcagctc agctatctat tccaagtatg ctaatggcat
     1381 agaaattcag ctgaaaaagg cctatgagcg catccaaggc tttgagagtg tacaggtcac
     1441 tcaattcagg aacgggagca tcgtggctgg ctatgaagtc gtcgggagtt ccagcgcttc
     1501 tgagctcctc tctgccattg agcatgttgc agaaaaagca aagacagccc tccataaact
     1561 ttttccactg gaagatgggt cctttagagt tttcggcaag gctcaatgta atgacatcgt
     1621 ttttggcttc ggttccaagg atgatgaata cacacttcca tgctcatccg gctatagggg
     1681 taacattacg gccaaatgcg agtcaagcgg gtggcaggtg attagggaga cctgtgtgct
     1741 gtcccttttg gaggaattga ataaaaattt tagcatgatc gtcgggaacg caacagaggc
     1801 tgccgtgagc tccttcgtgc aaaacctttc cgtgatcatt cgccagaatc ctagtaccac
     1861 agtcggcaac ctggcaagtg tggtgagcat actgagtaac atcagttccc tgagcttggc
     1921 aagtcacttc agggtgagta atagcaccat ggaggatgtc atatcaatcg cggataacat
     1981 tttgaattca gctagtgtga caaattggac cgttctgctg cgggaagaga aatacgcatc
     2041 ttcaagattg ctggaaactc tggagaatat aagcacactg gttccaccta ccgcgctgcc
     2101 tcttaacttt tctagaaagt tcattgattg gaagggcatt cctgtgaata agtcccagct
     2161 gaaaagaggt tactcttatc agattaagat gtgccctcag aacacatcca tccctatacg
     2221 aggcagggta cttatcggct cagatcaatt ccaacgatcc ctgcccgaaa ccatcatctc
     2281 catggcctct ctgacgctgg gcaacatact cccagtgtcc aaaaacggga atgcccaggt
     2341 gaatggccct gtcatttcta ccgtcatcca gaactattct atcaatgaag tgtttctgtt
     2401 tttttccaaa atcgaatcaa atttgtctca gcctcattgt gtgttctggg acttctctca
     2461 tctccagtgg aacgatgcag gatgtcattt ggtgaacgag actcaggaca tcgttacctg
     2521 tcaatgtaca cacctcacta gtttcagtat actcatgtca cccttcgtgc cgtccaccat
     2581 tttcccagtt gttaaatgga ttacttacgt gggactgggc atctccattg gaagcctcat
     2641 cctctgcttg atcatcgaag ctctcttttg gaagcagatc aaaaaatctc agacctctca
     2701 tacacgccga atttgtatgg tgaatatcgc cttgtccctt ctgatcgccg atgtttggtt
     2761 catcgtcgga gccaccgtgg ataccaccgt taacccttcc ggcgtgtgta ctgcggcagt
     2821 cttcttcact cacttcttct acctgtcact gttcttctgg atgctcatgt tgggcatcct
     2881 cctggcttat cggatcatcc tggtctttca ccacatggct caacacctga tgatggctgt
     2941 ggggttctgc ctcggatacg gttgcccact cattataagc gttatcacca tagcagtgac
     3001 acagccatcc aacacttaca aacggaaaga tgtctgctgg ctgaactggt ctaacggcag
     3061 taaaccactt ctggccttcg tcgtccctgc cctcgcaatt gtggctgtga acttcgtggt
     3121 ggtgctcctc gtcctcacca aactttggcg ccccacggtg ggagagcgcc tgagccgaga
     3181 tgacaaggcg actataatta gggtcggcaa gtctttgctg atcctcaccc ccctgctggg
     3241 gttgacctgg ggattcggca ttggtaccat agtggacagc caaaacttgg cttggcatgt
     3301 aatcttcgcg ctcttgaatg ctttccaggg gtttttcatc ctgtgctttg gaatcctgct
     3361 cgatagtaaa ctgcgccagc tcctgttcaa taagctgagt gctcttagtt cttggaaaca
     3421 gaccgaaaag cagaactcat ctgatctgtc tgcaaaacca aagttctcca agccgtttaa
     3481 tcctcttcag aataagggcc actacgcatt cagccacaca ggcgacagtt cagacaacat
     3541 catgctgact cagtttgtca gcaatgaaat cgataccggt ggacgcaccc cacccagcct
     3601 gggtccccaa gatgagtcct gcaccaccgc cagctcctcc ctggccaagg acacttcatc
     3661 gaccggtgag aacctgtact tccagctaag attagataaa agtaaagtga ttaacagcgc
     3721 attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa
     3781 gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga
     3841 cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga
     3901 aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca
     3961 tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct
     4021 cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc
     4081 actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt
     4141 cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc
     4201 tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat
     4261 catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tccgcgtaca gccgcgcgcg
     4321 tacgaaaaac aattacgggt ctaccatcga gggcctgctc gatctcccgg acgacgacgc
     4381 ccccgaagag gcggggctgg cggctccgcg cctgtccttt ctccccgcgg gacacacgcg
     4441 cagactgtcg acggcccccc cgaccgatgt cagcctgggg gacgagctcc acttagacgg
     4501 cgaggacgtg gcgatggcgc atgccgacgc gctagacgat ttcgatctgg acatgttggg
     4561 ggacggggat tccccgggtc cgggatttac cccccacgac tccgccccct acggcgctct
     4621 ggatatggcc gacttcgagt ttgagcagat gtttaccgat gcccttggaa ttgacgagta
     4681 cggtgggtag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     4741 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     4801 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     4861 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     4921 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     4981 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5041 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5101 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5161 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5221 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5281 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5341 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5401 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     5461 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5521 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5581 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5641 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5701 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5761 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
     5821 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     5881 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5941 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     6001 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     6061 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     6121 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     6181 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     6241 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     6301 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     6361 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     6421 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     6481 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     6541 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     6601 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     6661 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     6721 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     6781 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     6841 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6901 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     6961 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7021 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7081 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7141 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7201 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7261 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7321 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7381 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7441 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7501 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7561 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7621 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7681 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7741 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7801 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7861 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7921 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7981 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8041 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8101 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8161 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8221 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8281 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8341 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8401 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8461 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8521 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8581 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8641 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8701 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8761 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8821 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8881 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8941 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9001 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9061 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9121 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     9181 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     9241 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     9301 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     9361 gcgtt
//
