LOCUS       sequence-31417-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DLC1A
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             746..763
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(816..834)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      830..877
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat cacagcagcg
       61 gcagagaaaa cttgtatttc cagggcagtg tgtttggggt cccactgacg gtcaacgtgc
      121 agcgcacagg acaaccgttg cctcagagca tccagcaggc catgcgatac ctccggaacc
      181 attgtttgga tcaggttggg ctcttcagaa aatcgggggt caagtcccgg attcaggctc
      241 tgcgccagat gaatgaaggt gccatagact gtgtcaacta cgaaggacag tctgcttatg
      301 acgtggcaga catgctgaag cagtattttc gagatcttcc tgagccacta atgacgaaca
      361 aactctcgga aacctttcta cagatctacc aatatgtgcc caaggaccag cgcctgcagg
      421 ccatcaaggc tgccatcatg ctgctgcctg acgagaaccg ggaggttctg cagaccctgc
      481 tttatttcct gagcgatgtc acagcagccg taaaagaaaa ccagatgacc ccaaccaacc
      541 tggccgtgtg cttagcgcct tccctcttcc atctcaacac cctgaagaga gagaattcct
      601 ctcccagggt aatgcaaaga aaacaaagtt tgggcaaacc agatcagaaa gatttgaatg
      661 aaaacctagc tgccactcaa gggctggccc atatgatcgc cgagtgcaag aagctttgat
      721 gacgaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      781 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      841 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatat
//
