LOCUS       sequence-314170-       12004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A fusion of zebrafish synaptophysin to GFP and DsRedExpress under 
            the control of separate UAS cassettes; for dual-labeling of retinal 
            ganglion cell (RGC) axon arbors in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12004)
  AUTHORS   Meyer MP, Smith SJ
  TITLE     Evidence from in vivo imaging that synaptogenesis guides the growth 
            and branching of axonal arbors by two distinct mechanisms.
  JOURNAL   J Neurosci. 2006 Mar 29;26(13):3604-14.
  PUBMED    16571769
REFERENCE   2  (bases 1 to 12004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2006-03-29"; volume: "26(13)"; pages: "3604-14"
COMMENT     Sequence Label: 5UAS zfSynaptophysin:GFP-5UAS DsRedExpress
FEATURES             Location/Qualifiers
     source          1..12004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..239
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     186..207
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     regulatory      266..275
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1193..1202
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1199..1201,1202..1204,1205..1918)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1199..1201,1202..1204,1205..1915)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1244..1265)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1505..1524)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1852..1873
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          2025..2090
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2220..2240
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2512..2646
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2673..2691)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3143..3214,3616..3889,3964..4618,4680..4995,5199..5330,
                     5401..6015)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     regulatory      7816..7825
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7822..8499
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(7952..7970)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(8002..8022)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8220..8239
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8414..8437
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    8620..8741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(8748..9203)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8885..8906)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9097..9116
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9230..9334
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9336..9693
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9336..9527
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9359..9379)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9544..9679
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9728..10522
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9728..10522
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9782..9801)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10392..10411
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10710..10729)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10754..10801
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11130..11718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11619..11638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    11892..11986
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag
       61 tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca
      121 atctgcagta aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat
      181 caactgcaac tactgaaatc tgccaagaag taattattga atacaagaag agaactctga
      241 atagggaatt gggaattcgc ccttcgccac catggatgtt gccaaccagt tggtcgccac
      301 tgggcataca atcgtcaaac agccattagg attcatcaaa atcttacaat ggttctttgc
      361 catcttcgcg ttcgccacct gcggtggtta ctctggagtg tttcgtatga gcgtggagtg
      421 tgagaaccgc tcagagagtg acctgaacat tgaggttgag tttgcgtacc cgttcagact
      481 gcaccaggta tggtttgatg cccctacgtg taaaggatca gaacccgagc gactctttct
      541 ggtgggagac aactcttcct cagcagagtt cttcgtcacc atcggcgtct tctccttcct
      601 ttattcaatg gcagccattt ctgtgtacat cttcctgttg gagaaatacc gcgagggaaa
      661 caaaggagct caggctgatt tcattgtgac agcggttttc accttcatgt ggctggtgag
      721 ttcatcggcg tgggctaaag gtctctcaga cgtgaagaga gccaccgacc ctgatgacgt
      781 tatcaacctc atccctgcgt gcgaacgaga ggaaaaccga tgcaaagagc tgcacgaacc
      841 ggtggtgtct ggcctgaaca catctgtggc attcgggttc tgtaatgtga tcctatgggg
      901 aggcaacctc tggttcatct tcaaggaaac cggctggatg gcggctttcg ctggcaccta
      961 catgccatct ggagagaagc agccagctcc agactccttc ggccagggct atggacagga
     1021 aggttatgga caggaccagt atgccagctc tcagggtgga tatcagcctg actacggcca
     1081 gcagggtgga tatgaaggcg gtggatacaa tcaaggagtg tatggtcagg gagagcccac
     1141 atccttctcc aacgagatgt gtggaggagg acccgggatc caccggccgg tcgccaccat
     1201 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1261 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1321 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1381 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1441 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1501 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1561 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1621 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1681 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1741 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1801 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1861 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag
     1921 cggccgcggc tcgagggtac ctctagagga tctttgtgaa ggaaccttac ttctgtggtg
     1981 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     2041 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     2101 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     2161 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     2221 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     2281 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     2341 aaaaagctgc actgctatac aagaaaatta tggaaaaata tttgatgtat agtgccttga
     2401 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     2461 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     2521 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     2581 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     2641 tggatccact agaaggccta attctagtat gtatgtaagt taataaaacc catttttgcg
     2701 gaaagtagat aaaaaaaaca tttttttttt ttactgcact ggatatcatt gaacttatct
     2761 gatcagtttt aaatttactt cgatccaagg gtatttgatg taccaggttc tttcgattac
     2821 ctctcactca aaatgacatt ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac
     2881 agaaatatcg ccgtctcttt cgccgctgcg tccgctatct ctttcgccac cgtttgtagc
     2941 gttacgtagc gtcaatgtcc gccttcagtt gcattttgtc agcggtttcg tgacgaagct
     3001 ccaagcggtt tacgccatca attaaacaca aagtgctgtg ccaaaactcc tctcgcttct
     3061 tatttttgtt tgttttttga gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg
     3121 gaaagtgtga aaaatcccgg caatgggcca agaggatcag gagctattaa ttcgcggagg
     3181 cagcaaacac ccatctgccg agcatctgaa caatgtgagt agtacatgtg catacatctt
     3241 aagttcactt gatctatagg aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac
     3301 tgcgacccac aaaaatccca aaccgcaatt gcacaaacaa atagtgacac gaaacagatt
     3361 attctggtag ctgttctcgc tatataagac aatttttgag atcatatcat gatcaagaca
     3421 tctaaaggca ttcattttcg actatattct tttttacaaa aaatataaca accagatatt
     3481 ttaagctgat cctagatgca caaaaaataa ataaaagtat aaacctactt cgtaggatac
     3541 ttcggggtac tttttgttcg gggttagatg agcataacgc ttgtagttga tatttgagat
     3601 cccctatcat tgcagggtga cagcggagcg gcttcgcaga gctgcattaa ccagggcttc
     3661 gggcaggcca aaaactacgg cacgctccgg ccacccagtc cgccggagga ctccggttca
     3721 gggagcggcc aactagccga gaacctcacc tatgcctggc acaatatgga catctttggg
     3781 gcggtcaatc agccgggctc cggatggcgg cagctggtca accggacacg cggactattc
     3841 tgcaacgagc gacacatacc ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta
     3901 ttcgcagtcg gctgatctgt gtgaaatctt aataaagggt ccaattacca atttgaaact
     3961 cagtttgcgg cgtggcctat ccgggcgaac ttttggccgt gatgggcagt tccggtgccg
     4021 gaaagacgac cctgctgaat gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc
     4081 catccgggat gcgactgctc aatggccaac ctgtggacgc caaggagatg caggccaggt
     4141 gcgcctatgt ccagcaggat gacctcttta tcggctccct aacggccagg gaacacctga
     4201 ttttccaagc catggtgcgg atgccacgac atctgaccta tcggcagcga gtggcccgcg
     4261 tggatcaggt gatccaggag ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc
     4321 ccggcagggt gaaaggtctg tccggcggag aaaggaagcg tctggcattc gcctccgagg
     4381 ctctaaccga tccgccgctt ctgatctgcg atgagcccac ctccggactg gactccttta
     4441 ccgcccacag cgtcgtccag gtgctgaaga agctgtcgca gaagggcaag accgtcatcc
     4501 tgaccattca tcagccgtct tccgagctgt ttgagctctt tgacaagatc cttctgatgg
     4561 ccgagggcag ggtagctttc ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt
     4621 gagttcgatg tgtttattaa gggtatctag tattacataa catctcaact cctatccagc
     4681 gtgggtgccc agtgtcctac caactacaat ccggcggact tttacgtaca ggtgttggcc
     4741 gttgtgcccg gacgggagat cgagtcccgt gatcggatcg ccaagatatg cgacaatttt
     4801 gccattagca aagtagcccg ggatatggag cagttgttgg ccaccaaaaa tctggagaag
     4861 ccactggagc agccggagaa tgggtacacc tacaaggcca cctggttcat gcagttccgg
     4921 gcggtcctgt ggcgatcctg gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga
     4981 cttattcaga caacggtgag tggttccagt ggaaacaaat gatataacgc ttacaattct
     5041 tggaaacaaa ttcgctagat tttagataga attgcctgat tccacaccct tcttagtttt
     5101 tttcaatgag atgtatagtt tatagttttg cagaagataa ataaatttca tttaactcgc
     5161 gaatattaat gagatgcgag taacatttta atttgcagat ggttgccatc ttgattggcc
     5221 tcatcttttt gggccaacaa ctcacgcaag tgggtgtgat gaatatcaac ggagccatct
     5281 tcctcttcct gaccaacatg acctttcaaa acgtctttgc cacgataaat gtaagtcatg
     5341 tttagaatac atttgcattt caataattta ctaactttct aatgaatcga ttcgatttag
     5401 gtgttcacct cagagctgcc agtttttatg agggaggccc gaagtcgact ttatcgctgt
     5461 gacacatact ttctgggcaa aacgattgcc gaattgccgc tttttctcac agtgccactg
     5521 gtcttcacgg cgattgccta tccgatgatc ggactgcggg ccggagtgct gcacttcttc
     5581 aactgcctgg cgctggtcac tctggtggcc aatgtgtcaa cgtccttcgg atatctaata
     5641 tcctgcgcca gctcctcgac ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca
     5701 ttcctgctct ttggcggctt cttcttgaac tcgggctcgg tgccagtata cctcaaatgg
     5761 ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg
     5821 gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc
     5881 aaggtcatcc tggagacgct taacttctcc gccgccgatc tgccgctgga ctacgtgggt
     5941 ctggccattc tcatcgtgag cttccgggtg ctcgcatatc tggctctaag acttcgggcc
     6001 cgacgcaagg agtagccgac atatatccga aataactgct tgtttttttt ttttaccatt
     6061 attaccatcg tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc
     6121 caataaaaac aagatatgac ctatagaata caagtatttc cccttcgaac atccccacaa
     6181 gtagactttg gatttgtctt ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa
     6241 aactcgttta tcgctacata aaacaccggg atatattttt tatatacata cttttcaaat
     6301 cgcgcgccct cttcataatt cacctccacc acaccacgtt tcgtagttgc tctttcgctg
     6361 tctcccaccc gctctccgca acacattcac cttttgttcg acgaccttgg agcgactgtc
     6421 gttagttccg cgcgattcgg ttcgctcaaa tggttccgag tggttcattt cgtctcaata
     6481 gaaattagta ataaatattt gtatgtacaa tttatttgct ccaatatatt tgtatatatt
     6541 tccctcacag ctatatttat tctaatttaa tattatgact ttttaaggta attttttgtg
     6601 acctgttcgg agtgattagc gttacaattt gaactgaaag tgacatccag tgtttgttcc
     6661 ttgtgtagat gcatctcaaa aaaatggtgg gcataatagt gttgtttata tatatcaaaa
     6721 ataacaacta taataataag aatacattta atttagaaaa tgcttggatt tcactggaac
     6781 tagaattaat tcggctgctg ctctaaacga cgcatttcgt actccaaagt acgaattttt
     6841 tccctcaagc tcttattttc attaaacaat gaacaggacc taacgcacag tcacgttatt
     6901 gtttacataa atgatttttt ttactattca aacttactct gtttgtgtac tcccactggt
     6961 atagccttct tttatctttt ctggttcagg ctctatcact ttactaggta cggcatctgc
     7021 gttgagtcgc ctccttttaa atgtctgacc ttttgcaggt gcagccttcc actgcgaatc
     7081 attaaagtgg gtatcacaaa tttgggagtt ttcaccaagg ctgcacccaa ggctctgctc
     7141 ccacaatttt ctcttaatag cacacttcgg cacgtgaatt aattttactc cagtcacagc
     7201 tttgcagcaa aatttgcaat atttcatttt tttttattcc acgtaagggt taatgttttc
     7261 aaaaaaaaat tcgtccgcac acaacctttc ctctcaacaa gcaaacgtgc actgaattta
     7321 agtgtatact tcggtaagct taggcctcca aggcggagta ctgtcctccg ggctggcgga
     7381 gtactgtcct ccggcaaggt cggagtactg tcctccgaca ctagaggtcg gagtactgtc
     7441 ctccgacgca aggcggagta ctgtcctccg ggctgcggag tactgtcctc cggcaaggtc
     7501 ggagtactgt cctccgacac tagaggtcgg agtactgtcc tccgacgcaa ggtcggagta
     7561 ctgtcctccg acactagagg tcggagtact gtcctccgac gcaaggtcgg agtactgtcc
     7621 tccgacacta gaggtcggag tactgtcctc cgacgcaagg cggagtactg tcctccgggc
     7681 tggcggagta ctgtcctccg gcaagggtcg actctagagg gtatataatg gatcccatcg
     7741 cgtctcagcc tcactttgag ctcctccaca cgaattctgc agtcgacggt accgcgggcc
     7801 cgggatccac cggtcgccac catggcctcc tccgaggacg tcatcaagga gttcatgcgc
     7861 ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     7921 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     7981 ctgcccttcg cctgggacat cctgtccccc cagttccagt acggctccaa ggtgtacgtg
     8041 aagcaccccg ccgacatccc cgactacaag aagctgtcct tccccgaggg cttcaagtgg
     8101 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     8161 caggacggct ccttcatcta caaggtgaag ttcatcggcg tgaacttccc ctccgacggc
     8221 cccgtaatgc agaagaagac tatgggctgg gaggcctcca ccgagcgcct gtacccccgc
     8281 gacggcgtgc tgaagggcga gatccacaag gccctgaagc tgaaggacgg cggccactac
     8341 ctggtggagt tcaagtccat ctacatggcc aagaagcccg tgcagctgcc cggctactac
     8401 tacgtggact ccaagctgga catcacctcc cacaacgagg actacaccat cgtggagcag
     8461 tacgagcgcg ccgagggccg ccaccacctg ttcctgtagc ggccgcgact ctagatcata
     8521 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     8581 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     8641 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8701 cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt
     8761 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     8821 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     8881 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     8941 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     9001 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     9061 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     9121 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     9181 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     9241 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     9301 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     9361 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9421 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     9481 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9541 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9601 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9661 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     9721 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     9781 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     9841 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     9901 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     9961 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
    10021 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
    10081 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
    10141 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
    10201 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
    10261 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
    10321 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
    10381 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
    10441 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
    10501 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
    10561 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
    10621 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
    10681 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
    10741 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
    10801 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
    10861 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
    10921 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
    10981 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    11041 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    11101 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    11161 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    11221 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    11281 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    11341 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    11401 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    11461 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    11521 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    11581 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    11641 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    11701 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    11761 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    11821 accgccatgc attagttatt actagcgcta ccggactcag atctcgagct caagcttgca
    11881 tgcctgcagg tcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt
    11941 cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactctagcg
    12001 agcg
//
