LOCUS       sequence-314177-        1260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  homologous donor template for endogenous tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Gu B, Comerci CJ, McCarthy DG, Saurabh S, Moerner WE, Wysocka J
  TITLE     Opposing Effects of Cohesin and Transcription on CTCF Organization 
            Revealed by Super-resolution Imaging.
  JOURNAL   Mol Cell. 2020 Nov 19;80(4):699-711.e7. doi: 
            10.1016/j.molcel.2020.10.001. Epub 2020 Oct 21.
  PUBMED    33091336
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.10.001"; journalName: "Mol Cell"; date: 
            "2020-11-19- 19"; volume: "80"; issue: "4"; pages: "699-711.e7"
COMMENT     Sequence Label: pGEMT-blastR-p2a-v5-dL5
FEATURES             Location/Qualifiers
     source          1..1260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..396
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             406..462
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             469..510
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             511..1230
                     /codon_start=1
                     /product="fluorogen-activating protein composed of a dimer 
                     of the L5-MG single-chain antibody fragment (Szent-Gyorgyi 
                     et al., 2008)"
                     /label=FAP-alpha-2
                     /note="binds malachite green derivatives to produce red 
                     fluorescence"
                     /translation="QAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGHYANWFQQKPGQA
                     PRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSLSDVDGYLFGGGT
                     QLTVLSGGGGSGGGGSGGGGSGGGGSQAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGH
                     YANWFQQKPGQAPRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSL
                     SDVDGYLFGGGTQLTVLS"
ORIGIN
        1 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
       61 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
      121 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
      181 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
      241 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
      301 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
      361 cgtgaattgc tgccctctgg ttatgtgtgg gagggcggaa gcggagctac taacttcagc
      421 ctgctgaagc aggctggaga cgtggaggag aaccctggac ctaagcttgg taagccgatt
      481 ccaaacccac tgctgggact ggatagcaca caggccgtcg ttacccaaga acctagtgtt
      541 accgttagcc caggtggtac tgttatactt acttgtggaa gtggtacggg tgccgtcaca
      601 tctggtcatt atgcaaattg gtttcaacaa aaaccaggac aagctccaag agctttgatt
      661 tttgatactg ataagaagta ttcttggacc ccaggtagat tttctggatc tttgctggga
      721 gcaaaggcag ctttgacaat atcagatgct cagcctgagg acgaagccga gtattactgt
      781 tctcttagcg acgtggatgg ctacttgttt ggcggtggaa cacaactgac ggttctgtcc
      841 ggtggtggcg gctctggtgg cggtggcagc ggcggtggtg gttccggagg cggcggttct
      901 caggctgtgg tgactcagga gccgtcagtg actgtgtccc caggagggac agtcattctc
      961 acttgtggct ccggcactgg agctgtcacc agtggtcatt atgccaactg gttccagcag
     1021 aagcctggcc aagcccccag ggcacttata tttgacaccg acaagaagta ttcctggacc
     1081 cctggccgat tctcaggctc cctccttggg gccaaggctg ccctgaccat ctcggatgcg
     1141 cagcctgaag atgaggctga gtattactgt tcgctctccg acgttgacgg ttatctgttc
     1201 ggaggaggca cccagctgac cgtcctctcc ggaggaggtg ggtctggagg tggaggatct
//
