LOCUS       sequence-314183-        9446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ostir and G418 resistance gene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Gu B, Comerci CJ, McCarthy DG, Saurabh S, Moerner WE, Wysocka J
  TITLE     Opposing Effects of Cohesin and Transcription on CTCF Organization 
            Revealed by Super-resolution Imaging.
  JOURNAL   Mol Cell. 2020 Nov 19;80(4):699-711.e7. doi: 
            10.1016/j.molcel.2020.10.001. Epub 2020 Oct 21.
  PUBMED    33091336
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.10.001"; journalName: "Mol Cell"; date: 
            "2020-11-19- 19"; volume: "80"; issue: "4"; pages: "699-711.e7"
COMMENT     Sequence Label: PB-Ostir-neo
FEATURES             Location/Qualifiers
     source          1..9446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        3..382
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..660
                     /label=chicken beta-actin promoter
     intron          661..1669
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1593..1615
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1677..1696
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1748..3472
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             3473..3553
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             3563..3619
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3626..4417
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3677..3696)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4287..4306
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4445..4462)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4451..4658
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4665..4786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4702..4721)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4756..4775
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5548..5582
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6073..6089)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6073..6089)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6086..6108)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6097..6113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6121..6127,6128..6145,6146..6151))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6166..6187
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6304..6321)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6475..7063)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6555..6574)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7234..8025,8026..8094))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7857..7876
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8095..8199)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8225..8680)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8362..8383)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8574..8593
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8807..8829
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8821..8838
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8822..8838
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8968..9030
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
       61 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      121 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      181 gactttccat tgacgtcaat gggtggacta tttacggtaa actgcccact tggcagtaca
      241 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      301 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      361 attagtcatc gctattacca tgggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
      541 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgttgccttc gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc
      841 cttaaagggc tccgggaggg ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt
      901 gtgtgtgtgc gtggggagcg ccgcgtgcgg cccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc gtgtgcgcga ggggagcgcg gccgggggcg
     1021 gtgccccgcg gtgcgggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     1081 gggggggtga gcagggggtg tgggcgcggc ggtcgggctg taaccccccc ctgcaccccc
     1141 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtgcg gggcgtggcg
     1201 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
     1261 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg ccccggagcg ccggcggctg
     1321 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     1381 acttcctttg tcccaaatct ggcggagccg aaatctggga ggcgccgccg caccccctct
     1441 agcgggcgcg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     1501 gtgcgtcgcc gcgccgccgt ccccttctcc atctccagcc tcggggctgc cgcaggggga
     1561 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     1621 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     1681 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcctcgaga attctcacgc
     1741 gtccaccatg acatacttcc ctgaggaggt ggtcgaacat attttctcat tccttcccgc
     1801 ccaacgagat aggaataccg tgtccctggt gtgcaaggtc tggtatgaga tcgaacgctt
     1861 gagccgcaga ggtgtttttg tgggtaattg ttatgccgta cgagccgggc gagtggccgc
     1921 ccggttcccg aacgtcaggg cccttacagt gaagggcaag cctcactttg ccgacttcaa
     1981 tttggtgcct ccggactggg gcggctacgc cgggccctgg atcgaggccg ctgcgcgcgg
     2041 gtgtcatggt ctggaagaac tcaggatgaa aaggatggtg gtgtcagacg agtcactgga
     2101 actgcttgcc cgcagcttcc cccgattccg cgccctggtg ctgatcagct gtgaaggatt
     2161 tagcaccgac ggtctggctg ctgtagctag ccattgtaaa ctgctgcgag aactggacct
     2221 gcaggaaaat gaggtagagg ataggggacc tcggtggctg agctgtttcc cggatagctg
     2281 cactagcctg gtgagcttga atttcgcttg tatcaagggc gaagtgaatg ccgggagttt
     2341 ggagagactc gtttcccgat cccccaacct gagatctctg cggctgaaca gatccgtttc
     2401 tgtggacacg ctcgccaaga ttttgctccg gactcccaac ctggaggatc tggggaccgg
     2461 aaatctgacc gatgatttcc agacagagag ctactttaag ctcactagtg ctctggaaaa
     2521 atgcaaaatg cttaggtcac tgagcgggtt ttgggacgct agccctgtct gcctgtcttt
     2581 tatctatccc ctgtgtgcac agttgaccgg acttaatctg agttacgctc caaccctcga
     2641 cgctagtgac cttactaaga tgatcagccg ctgcgtgaag ctccaaaggc tctgggtact
     2701 ggattgtatt tcagacaagg gactgcaagt tgtggcctcc tcctgcaaag atctgcagga
     2761 gctgagggtg ttcccttcag atttctatgt ggccggatac agcgcagtca ccgaggaggg
     2821 attggtagca gtgtccctgg ggtgccccaa actcaatagt ttgctgtact tctgtcatca
     2881 gatgaccaat gctgcccttg ttaccgttgc caaaaactgt ccaaacttca caaggtttag
     2941 actgtgcatc ttggagcccg gtaagcccga cgtcgtaacg tcacagccac tggacgaagg
     3001 gttcggtgcc atagtacgcg aatgtaaggg tctccagcgg ctgagcatat ccggcctgct
     3061 gactgacaaa gtgttcatgt atattgggaa gtacgccaag cagctggaaa tgctgtcaat
     3121 cgcattcgcc ggagattcag ataaagggat gatgcacgtg atgaacgggt gcaagaacct
     3181 gagaaaactc gaaattcgcg attccccttt tggggacgcc gcactgctgg gcaatttcgc
     3241 ccggtacgag acgatgaggt cactttggat gtcaagctgc aacgtgaccc tcaagggatg
     3301 ccaagtcctg gctagtaaaa tgccaatgct gaatgttgag gtcatcaacg aacgcgacgg
     3361 tagtaatgaa atggaggaaa accacggcga tctgcccaaa gtggagaaac tgtacgtcta
     3421 tcggaccaca gctggcgcac gagatgacgc tcccaatttc gtgaaaattc tgtacccata
     3481 cgatgttcca gattacgctt atccctacga cgtgcctgat tatgcatacc catatgatgt
     3541 ccccgactat gccggaagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt
     3601 ggaggagaac cctggaccta agcttattga acaagatgga ttgcacgcag gttctccggc
     3661 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     3721 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     3781 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     3841 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     3901 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     3961 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     4021 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     4081 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     4141 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     4201 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     4261 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     4321 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     4381 catcgccttc tatcgccttc ttgacgagtt cttctgagaa ttctaactag agctcgctga
     4441 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     4501 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     4561 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     4621 ggggaggatt gggaagagaa tagcaggcat gctggggagc ggccaacttg tttattgcag
     4681 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4741 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggaatt
     4801 gactcaaatg atgtcaatta gtctatcaga agctatctgg tctcccttcc gggggacaag
     4861 acatccctgt ttaatattta aacagcagtg ttcccaaact gggttcttat atcccttgct
     4921 ctggtcaacc aggttgcagg gtttcctgtc ctcacaggaa cgaagtccct aaagaaacag
     4981 tggcagccag gtttagcccc ggaattgact ggattccttt tttagggccc attggtatgg
     5041 ctttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga
     5101 ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg
     5161 gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta
     5221 gcgggggagg gacgtaatta catccctggg ggctttgggg gggggctgtc cctgatatct
     5281 ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa ataacaatat
     5341 aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg tcattttgac
     5401 tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag cacgcctcac
     5461 gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc gcgctattta
     5521 gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact atctttctag
     5581 ggttaatcta gctgcatcag gatcatatcg tcgggtcttt tttccggctc agtcatcgcc
     5641 caagctggcg ctatctgggc atcggggagg aagaagcccg tgccttttcc cgcgaggttg
     5701 aagcggcatg gaaagagttt gccgaggatg actgctgctg cattgacgtt gagcgaaaac
     5761 gcacgtttac catgatgatt cgggaaggtg tggccatgca cgcctttaac ggtgaactgt
     5821 tcgttcaggc cacctgggat accagttcgt cgcggctttt ccggacacag ttccggatgg
     5881 tcagcccgaa gcgcatcagc aacccgaaca ataccggcga cagccggaac tgccgtgccg
     5941 gtgtgcagat taatgacagc ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc
     6001 ctggctggat gccgcagaaa tggacatgga taccccgtga gttacccggc gggcgcgctt
     6061 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6121 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6181 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6241 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6301 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6361 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6421 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6481 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6541 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6601 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6661 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6721 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6781 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6841 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6901 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6961 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7021 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7081 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7141 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7201 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7261 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7321 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7381 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7441 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7501 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7561 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7621 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7681 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7741 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7801 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7861 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7921 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7981 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8041 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8101 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8161 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8221 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     8281 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     8341 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     8401 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     8461 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     8521 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     8581 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     8641 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     8701 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     8761 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     8821 tgtaaaacga cggccagtga gcgcgcctcg ttcattcacg tttttgaacc cgtggaggac
     8881 gggcagactc gcggtgcaaa tgtgttttac agcgtgatgg agcagatgaa gatgctcgac
     8941 acgctgcaga acacgcagct agattaaccc tagaaagata atcatattgt gacgtacgtt
     9001 aaagataatc atgcgtaaaa ttgacgcatg tgttttatcg gtctgtatat cgaggtttat
     9061 ttattaattt gaatagatat taagttttat tatatttaca cttacatact aataataaat
     9121 tcaacaaaca atttatttat gtttatttat ttattaaaaa aaaacaaaaa ctcaaaattt
     9181 cttctataaa gtaacaaaac ttttatgagg gacagccccc ccccaaagcc cccagggatg
     9241 taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc
     9301 cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag
     9361 gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct
     9421 ggggggatac ggggaaaagg cctcca
//
