LOCUS       sequence-314209-        3512 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector containing a PIP-binding defective 
            mutant of SNX9. Also contains mCherry tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, 
            McGarry L, Shanks E, Mostov KE, Bryant DM
  TITLE     The phospholipid PI(3,4)P2 is an apical identity determinant.
  JOURNAL   Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8.
  PUBMED    30487552
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-11-28"; volume: "9(1):5041. doi"; pages: " 
            10.1038/s41467-018-07464-8"
COMMENT     Sequence Label: mCherry-C1 SNX9 RYK mutant R286A Y287A K288A
FEATURES             Location/Qualifiers
     source          1..3512
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..1320
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(758..776)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1026..1045
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3294..3415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3331..3350)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3385..3404
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctaccat ggccaccaag gctcgggtca tgtacgactt tgctgctgag
     1381 cctggaaaca atgagctgac cgttactgaa ggagaaatca tcacagttac aaacccgaat
     1441 gttggtggag gatggctgga aggaaagaac aacaaaggag agcaggggct tgttcctact
     1501 gactacgtgg aaattttacc aaacgatgga aaagatccgt tttcttgtgg gaattcagta
     1561 gccgaccaag ctttcctcga ttccctgaca gctagcacag ctcaaaccaa ctcatcttct
     1621 gccaacagca ataaccaggt tggtggtggc aatgacccct ggacagcctg gaatgctccc
     1681 aaacctggga actgggacag ctccgatgcc tggggctcca ggacagacgg aactagcgct
     1741 cagaggaaca gcagcgccaa caactgggac acgggctttg gccacccaca ggcctaccaa
     1801 ggaccagcaa ctggtgatga tgacgaatgg gatgaagact gggacgaccc caagtcctcc
     1861 tcaccttact tcaaggattc agaaccagct gaagcagggg gcatccagcg gggaaacagt
     1921 cgtgcgggtg cctcctccat gaagctgccc cttaacaaat ttcctggatt tgcaaaacct
     1981 ggcatggagc agtacttgtt ggccaagcag ctagcaaaac ccaaagagaa aatcgccatc
     2041 atcgttggag attatggccc tatgtgggtt tatcctacct ctacgtttga ctgtgtggta
     2101 gctgatcccc ggaaaggttc caaaatgtac ggtctgaata gctacattga gtatcagcta
     2161 acacccacta acactaaccg atctgtaaac cacagatata agcactttga ttggttatat
     2221 gagcgtctcc tggttaagtt tgggtctgcc attccgatcc cttctcttcc agacaagcag
     2281 gtcacaggtc gctttgaaga ggaatttatc aaaatgaata tggagcggct ccaggcctgg
     2341 atgaccagga tgtgccggca cccggtggtg tcagaaagtg aagttttcca gcagttcctg
     2401 aatttccgag atgagaagga atggaagact ggaaagagga aggctgagaa agacgaactg
     2461 gtgggagtga tgatattttc caccatggaa ccagaggctc ctgacttgga tttgatagaa
     2521 atagaacaga agtgtgacgc tgtggggaag ttcaccaagg ccatggatga cggcgtgaag
     2581 gagctgctga cggtggggca ggagcactgg aagcgctgca cagggccctt acccaaggag
     2641 tatcagaaga tagggaaggc tctgcagagc ttagcggcag tgttcagctc cagcggttat
     2701 caaggagaga ccgaccttaa cgatgcaatc acagaagctg ggaagacgta tgaggagatc
     2761 gccagccttg tggcagagca gccaaagaag gacctccact tcctgatgga gtgcaaccat
     2821 gagtacaaag gctttctcgg ctgcttcccg gacatcattg gtgcccacaa gggagcaata
     2881 gaaaaagtga aagaaagtga taagctggtt gcaactagta aaattactcc ccaagacaag
     2941 cagaccatgg tgaagagagt tggcaccatg tcttacgctc tgcaagctga gatgaatcac
     3001 tttcacagta accggatcta tgattacaac agtgtcatcc gcctctacct ggagcagcaa
     3061 gtgcagttct atgaaacaat cgcagaaaag ctgaggcagg ccctcagccg cttcccggtt
     3121 atgggttctt caggatcatc cgggtcgacg gtaccgcggg cccgggatcc accggatcta
     3181 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     3241 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     3301 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3361 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     3421 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     3481 ttttttaacc aataggccga aatcggcaaa at
//
