LOCUS       sequence-314214-        2775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector containing a phosphatase dead mutant of 
            INPP4B (C842A). Also contains mCherry tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, 
            McGarry L, Shanks E, Mostov KE, Bryant DM
  TITLE     The phospholipid PI(3,4)P2 is an apical identity determinant.
  JOURNAL   Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8.
  PUBMED    30487552
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-11-28"; volume: "9(1):5041. doi"; pages: " 
            10.1038/s41467-018-07464-8"
COMMENT     Sequence Label: pmCherry-C1-INPP4B C842A mutant
FEATURES             Location/Qualifiers
     source          1..2775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggaaatta aagaggaagg ggcatcagaa gaagggcagc actttcttcc tacagcccag
       61 gccaatgatc ccggggactg tcagttcaca agtatccaga agactccaaa tgaaccgcag
      121 ttggaattca tccttgcatg caaggatctc gtggctcctg tccgtgatcg taaactgaat
      181 acactggtgc agatctccgt aatccacccc gtggagcaga gtctgacaag atactccagc
      241 accgaaattg tggagggaac aagggaccca ctgtttttga ctggtgtcac attcccatct
      301 gagtatccca tctatgagga gaccaaaata aaactaacag tctatgatgt caaggataag
      361 tctcatgaca ccgttcgaac cagtgtccta ccagaacata aggatccccc gccagaagtt
      421 gggcgaagtt tcttgggcta tgccagtttt aaagtgggag agctgctgaa gtcaaaggag
      481 caattgctgg tcctgagcct gagaacttca gatggtggca aagtggttgg caccatagaa
      541 gtcagtgtcg tgaagatggg ggagattgag gatggggaag ccgaccacat caccacagat
      601 gtacagggac aaaagtgtgc cctggtatgt gaatgtacag ccccggaaag tgtgagcgga
      661 aaagataact tacctttttt gaattcagtg ttaaagaacc cagtatgtaa attatataga
      721 tttcccacat ctgacaataa gtggatgcga attcgagagc agatgtcaga gagcattctt
      781 tcctttcata ttcctaagga attgatttcc cttcacatta aagaagattt gtgcagaaac
      841 caggagataa aagaacttgg tgagctttct ccacattggg acaatctgcg aaaaaatgtc
      901 cttactcact gtgatcaaat ggtgaatatg taccaagaca ttctgacaga acttagcaag
      961 gaaacagggt cctctttcaa atcaagcagc agcaaaggag agaaaacatt agaatttgtt
     1021 ccaataaatc tacatctgca aagaatgcag gtacacagcc ctcacttgaa agatgctctc
     1081 tacgatgtca tcactgtggg agccccagct gcccattttc agggatttaa gaatggtggt
     1141 cttcggaagc tactccatag atttgaaaca gaaagaagaa ataccggata ccagtttatt
     1201 tactattcac ctgaaaacac agccaaagca aaggaagttc tcagcaacat caatcaacta
     1261 caacctctta tagcaaccca tgcagaccta ctgcttaatt ctgcaagcca gcattctcca
     1321 gacagcttga agaattcttt aaagatgctt tcagaaaaaa cagagctttt tgtacatgcc
     1381 ttcaaggatc aacttgtcag gagtgctctt ttagcactct acactgcaag gccaggaggc
     1441 attcttaaga agccaccctc tcctaagagc agcacagagg agagcagtcc ccaagaccaa
     1501 cccccagtga tgagagggca ggactccata ccacatcatt cagactatga tgaggaagag
     1561 tgggacaggg tgtgggccaa tgtggggaag agcctgaact gcattattgc tatggtggac
     1621 aaactgattg aaagagatgg tggcagtgaa ggcagtggcg gcaacaatga tggagaaaag
     1681 gaaccttcat taacagatgc cattccctct cacccaagag aggactggta tgaacagttg
     1741 tatcccctca tccttaccct gaaggactgc atgggagaag tggtgaaccg agccaagcag
     1801 tccctgacat ttgtgctcct tcaggaactt gcgtacagct tgccccagtg tctgatgctg
     1861 acgctaagaa gagacatcgt cttcagccaa gcacttgctg gattggtttg tggttttatc
     1921 atcaaattac agacaagtct gtatgaccca ggcttcctac agcagcttca cacagtgggg
     1981 ttgatagtac aatatgaagg actgctaagt acatacagcg atgaaattgg aatgctagag
     2041 gacatggccg ttggcatttc cgatttaaag aaagttgcat ttaaaataat tgaagccaaa
     2101 tccaatgatg tgttgccagt tataacagga agacgagaac attacgtggt agaggtcaag
     2161 cttccagcca gaatgtttga gtcactacct ctacagatta aagaaggaca gttgcttcat
     2221 gtgtatccag tactttttaa tgttggaatc aatgaacagc aaactctggc tgaaaggttt
     2281 ggagatgtct ctttgcaaga aagtattaat caggaaaact tcgaacttct acaagaatat
     2341 tacaagatat ttatggaaaa gatgcctcct gattatattt cacattttca ggaacaaaat
     2401 gatttaaaag cattgctaga aaatctcctt caaaatatcc aatccaaaaa aagaaagaat
     2461 gtagaaatta tgtggctggc tgcaacgatt tgccgcaaac tgaatggtat tcgtttcacc
     2521 tgtgctaaaa gtgccaaaga caggacatcg atgtcagtga cacttgaaca atgctcaatc
     2581 ttgagagatg agcaccagtt acacaaggac ttctttatcc gagcgctgga ttgcatgaga
     2641 agagaaggat gccgcataga gaatgtactg aagaatatca aatgcagaaa gtatgctttc
     2701 aacatgctac agctgatggc tttccccaag tactacagac ctccagaggg gacttatgga
     2761 aaagctgaca cctaa
//
