LOCUS       sequence-314215-        9185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-responsive retroviral expression vector. Contains INPP5E. Also 
            contains SGFP tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, 
            McGarry L, Shanks E, Mostov KE, Bryant DM
  TITLE     The phospholipid PI(3,4)P2 is an apical identity determinant.
  JOURNAL   Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8.
  PUBMED    30487552
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-11-28"; volume: "9(1):5041. doi"; pages: " 
            10.1038/s41467-018-07464-8"
COMMENT     Sequence Label: pRevTRE SGFP2 Inpp5E WT shRNA resistant
FEATURES             Location/Qualifiers
     source          1..9185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             414..589
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1398..1420
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1436..1452
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1524..2543
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             1524..2543
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     protein_bind    join(2847..2865,2866..2888,2889..2907,2908..2930,2931..2949,
                     2950..2972,2973..2991,2992..3014,3015..3033,3034..3056,
                     3057..3075,3076..3098,3099..3117)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        3150..3187
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3184..3208
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3322..3331
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3328..3330,3331..3333,3334..4044)
                     /codon_start=1
                     /product="strongly enhanced green fluorescent protein 
                     (Kremers et al., 2007)"
                     /label=SGFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3634..3653)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3981..4002
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6087..6680
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6783..6805)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6891..7031
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6946..6965
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7046..7063)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7217..7805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7297..7316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7976..8767,8768..8836))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8599..8618
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8837..8941)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9009..9027
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aattccgatc tgatagcttg ccacaacccg
     1501 taccaaagat ggatagatcc ggaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     1561 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     1621 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     1681 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     1741 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     1801 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     1861 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     1921 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     1981 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     2041 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     2101 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     2161 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     2221 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     2281 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     2341 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     2401 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     2461 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     2521 ctcgtccgag ggcaaaggaa tagagtagat gccgaccgaa caagagctga tttcgagaac
     2581 gcctcagcca gcaactcgcg cgagcctagc aaggcaaatg cgagagaacg gccttacgct
     2641 tggtggcaca gttctcgtcc acagttcgct aagctcgctc ggctgggtcg cgggagggcc
     2701 ggtcgcagtg attcaggccc ttctggattg tgttggtccc cagggcacga ttgtcatgcc
     2761 cacgcactcg ggtgatctga ctgatcccgc agattggaga tcgccgcccg tgcctgccga
     2821 ttgggtgcag atctcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2881 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2941 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     3001 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     3061 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     3121 gtgaaagtcg agctcggtac ccgggtcgag taggcgtgta cggtgggagg cctatataag
     3181 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     3241 ccatagaaga caccgggacc gatccagcct ccgcggcccc gaattcgagc tcggtacccg
     3301 gggatcctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     3361 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3421 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     3481 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     3541 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3601 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     3661 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3721 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3781 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     3841 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3901 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
     3961 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     4021 ctcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgaattct
     4081 gcagtcgacg gtaccatgcc gtccaaggcg gagaatctgc ggccctccga gccggccccg
     4141 cagccgccgg aagggaggac gctccaagga cagcttcccg gcgctccgcc ggcccagcgc
     4201 gcggggtccc cacccgatgc tccgggctcc gagagccccg cgcttgcctg cagcactccg
     4261 gccacgccca gcggcgagga cccgccagcc cgagcagcac ccatcgcccc gcggcccccc
     4321 gccaggcctc gactggagcg agccctgtcc ctggacgaca agggctggag gaggaggcgt
     4381 tttcgaggca gccaggagga cctggaagcc cggaatggga ccagtccctc caggggctca
     4441 gtgcagagcg agggccccgg ggcccccgcc cacagctgct ccccgccctg cctgagcacc
     4501 tccttgcagg aaatccccaa gtcccgcggg gtcctgagca gtgagagagg gagcccgtcc
     4561 tcggggggta accctctctc tggggtggcc agcagctccc cgaacctccc gcacagagac
     4621 gccgccgtgg cggggagctc gcccaggctg cccagcctgc tgcccccgcg cccaccgcct
     4681 gccctgagcc tggacatcgc ctccgactcc ctgaggacag caaacaaggt cgactcggat
     4741 cttgcagact acaagctccg cgcgcagccg ctcctggtgc gggcccacag cagcctgggc
     4801 cccggccggc cgcggagccc cctggcctgc gacgactgtt cccttcgctc ggccaaatcc
     4861 tccttcagcc tcctggcgcc catccgcagc aaggacgtcc gcagcaggag ttacctggag
     4921 ggcagcctcc tggccagcgg ggccctgttg ggggcggatg agctggcccg ctacttccca
     4981 gaccggaacg tggcactctt cgtggccacc tggaacatgc agggccagaa ggagctcccg
     5041 cccagcctgg acgagttcct gctcccagcc gaggccgact atgcccagga cctgtatgtc
     5101 atcggggtcc aggagggctg ttctgacagg cgggagtggg agactcgtct gcaggagacg
     5161 ctgggcccgc actatgtgct gctgtcctcg gcggcccacg gcgtgctcta catgtcgctc
     5221 ttcatccgca gggacctcat ctggttctgc tcagaggtgg agtgctccac ggtgaccaca
     5281 cgcatcgtgt ctcagatcaa gaccaagggg gccttgggca tcagcttcac cttttttggc
     5341 acttccttcc tcttcatcac gtcccacttc acctcaggtg acgggaaggt ggcggagcgg
     5401 ctgctggact acaccaggac tgtacaagcc ctggtcctgc ccagaaatgt gcccgacacc
     5461 aacccctatc gctccagcgc agcggacgtc accacccgct tcgatgaggt gttctggttt
     5521 ggagacttca acttccgcct gagtggcggg cgcacagtcg tggacgccct cctgtgccag
     5581 ggcctggtgg tggacgtgcc ggcgctgctg cagcacgacc agctcatccg ggagatgcgg
     5641 aagggatcca tcttcaaggg cttccaggag ccggacatcc acttcctccc atcatacaag
     5701 tttgacatcg ggaaggacac gtacgacagc acctccaagc agaggacgcc ctcatacacg
     5761 gaccgcgtct tgtacagaag ccgccacaag ggtgacatct gtcctgtgag ctactcttcc
     5821 tgccccggga tcaagacgtc cgaccaccgc cctgtgtatg gcctcttccg ggtgaaagtg
     5881 aggccggggc gagacaacat tccgttggca gctggcaaat ttgatagaga actgtactta
     5941 ctaggaatta aaagacggat ttcgaaggag attcagaggc agcaagcact acagagtcag
     6001 aactccagca ccatctgctc cgtttcttga agcttgttaa catcgataaa ataaaagatt
     6061 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     6121 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc
     6181 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     6241 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
     6301 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     6361 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     6421 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     6481 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     6541 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     6601 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     6661 gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac
     6721 caccgaccca ccaccgggag gtaagctggc tgcctcgcgc gtttcggtga tgacggtgaa
     6781 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     6841 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     6901 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     6961 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     7021 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7081 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7141 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7201 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7261 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7321 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7381 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7441 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7501 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7561 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7621 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     7681 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7741 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7801 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7861 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7921 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7981 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8041 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8101 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8161 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8221 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8281 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8341 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8401 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8461 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8521 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8581 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8641 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8701 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8761 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8821 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8881 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8941 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     9001 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattcata ccagatcacc
     9061 gaaaactgtc ctccaaatgt gtccccctca cactcccaaa ttcgcgggct tctgcctctt
     9121 agaccactct accctattcc ccacactcac cggagccaaa gccgcggccc ttccgtttct
     9181 ttgct
//
