LOCUS       sequence-31422-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GIMAP4
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             788..805
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(858..876)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      872..919
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tccctctgaa tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc
       61 acagcagcgg cagagaaaac ttgtatttcc agggcgggcc tggaaggcaa gagcccagaa
      121 attcccaatt gagaattgtg ttagtgggta aaaccggagc aggaaaaagt gcaacaggaa
      181 acagcatcct tggccggaaa gtgtttcatt ctggcactgc agcaaaatcc attaccaaga
      241 agtgtgagaa acgcagcagc tcatggaagg aaacagaact tgtcgtagtt gacacaccag
      301 gcattttcga cacagaggtg cccaatgctg aaacgtccaa ggagattatt cgctgcattc
      361 ttctgacctc cccagggcct catgctctgc ttctggtggt tccactgggc cgttacactg
      421 aggaggagca caaagccaca gagaagatcc tgaaaatgtt tggagagagg gctagaagtt
      481 tcatgattct catattcacc cggaaagatg acttaggtga caccaatttg catgactact
      541 taagggaagc tccagaagac attcaagact tgatggacat tttcggtgac cgctactgtg
      601 cgttaaacaa caaggcaaca ggcgctgagc aggaggccca gagggcacag ttgctgggcc
      661 tgatccagcg cgtggtgagg gagaacaagg aaggctgcta cactaatagg atgtaccaaa
      721 gggcggagga ggagatccag aagcaaacac aagcaatgtg atgacgaagc ttgcggccgc
      781 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      841 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      901 ggtcttgagg ggttttttgc tg
//
