LOCUS       sequence-314220-        3564 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector, containing a GFP tagged phosphatase 
            dead version of INPP5D (D673G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, 
            McGarry L, Shanks E, Mostov KE, Bryant DM
  TITLE     The phospholipid PI(3,4)P2 is an apical identity determinant.
  JOURNAL   Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8.
  PUBMED    30487552
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-11-28"; volume: "9(1):5041. doi"; pages: " 
            10.1038/s41467-018-07464-8"
COMMENT     Sequence Label: INPP5D-eGFP D673G mutant
FEATURES             Location/Qualifiers
     source          1..3564
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1594..1602
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atggtcccct gctggaacca tggcaacatc acccgctcca aggcggagga gctgctttcc
       61 aggacaggca aggacgggag cttcctcgtg cgtgccagcg agtccatctc ccgggcatac
      121 gcgctctgcg tgctgtatcg gaattgcgtt tacacttaca gaattctgcc caatgaagat
      181 gataaattca ctgttcaggc atccgaaggc gtctccatga ggttcttcac caagctggac
      241 cagctcatcg agttttacaa gaaggaaaac atggggctgg tgacccatct gcaataccct
      301 gtgccgctgg aggaagagga cacaggcgac gaccctgagg aggacacaga aagtgtcgtg
      361 tctccacccg agctgccccc aagaaacatc ccgctgactg ccagctcctg tgaggccaag
      421 gaggttcctt tttcaaacga gaatccccga gcgaccgaga ccagccggcc gagcctctcc
      481 gagacattgt tccagcgact gcaaagcatg gacaccagtg ggcttccaga agagcatctt
      541 aaggccatcc aagattattt aagcactcag ctcgcccagg actctgaatt tgtgaagaca
      601 gggtccagca gtcttcctca cctgaagaaa ctgaccacac tgctctgcaa ggagctctat
      661 ggagaagtca tccggaccct cccatccctg gagtctctgc agaggttatt tgaccagcag
      721 ctctccccgg gcctccgtcc acgtcctcag gttcctggtg aggccaatcc catcaacatg
      781 gtgtccaagc tcagccaact gacaagcctg ttgtcgtcca ttgaagacaa ggtcaaggcc
      841 ttgctgcacg agggtcctga gtctccgcac cggccctccc ttatccctcc agtcaccttt
      901 gaggtgaagg cagagtctct ggggattcct cagaaaatgc agctcaaagt cgacgttgag
      961 tctgggaaac tgatcattaa gaagtccaag gatggttctg aggacaagtt ctacagccac
     1021 aagaaaatcc tgcagctgat taagtcacag aaatttctga ataagttggt gatcttggtg
     1081 gaaacagaga aggagaagat cctgcggaag gaatatgttt ttgctgactc caaaaagaga
     1141 gaaggcttct gccagctcct gcagcagatg aagaacaagc actcagagca gccggagccc
     1201 gacatgatca ccatcttcat cggcacctgg aacatgggta acgccccccc tcccaagaag
     1261 atcacgtcct ggtttctctc caaggggcag ggaaagacgc gggacgactc tgcggactac
     1321 atcccccatg acatttacgt gatcggcacc caagaggacc ccctgagtga gaaggagtgg
     1381 ctggagatcc tcaaacactc cctgcaagaa atcaccagtg tgacttttaa aacagtcgcc
     1441 atccacacgc tctggaacat ccgcatcgtg gtgctggcca agcctgagca cgagaaccgg
     1501 atcagccaca tctgtactga caacgtgaag acaggcattg caaacacact ggggaacaag
     1561 ggagccgtgg gggtgtcgtt catgttcaat ggaacctcct tagggttcgt caacagccac
     1621 ttgacttcag gaagtgaaaa gaaactcagg cgaaaccaaa actatatgaa cattctccgg
     1681 ttcctggccc tgggcgacaa gaagctgagt ccctttaaca tcactcaccg cttcacgcac
     1741 ctcttctggt ttggggatct taactaccgt gtggatctgc ctacctggga ggcagaaacc
     1801 atcatccaga aaatcaagca gcagcagtac gcagacctcc tgtcccacga ccagctgctc
     1861 acagagagga gggagcagaa ggtcttccta cacttcgagg aggaagaaat cacgtttgcc
     1921 ccaacctacc gttttgagag actgactcgg gacaaatacg cctacaccaa gcagaaagcg
     1981 acagggatga agtacaactt gccttcctgg tgtggccgag tcctctggaa gtcttatccc
     2041 ctggtgcacg tggtgtgtca gtcttatggc agtaccagcg acatcatgac gagtgaccac
     2101 agccctgtct ttgccacatt tgaggcagga gtcacttccc agtttgtctc caagaacggt
     2161 cccgggactg ttgacagcca aggacagatt gagtttctca ggtgctatgc cacattgaag
     2221 accaagtccc agaccaaatt ctacctggag ttccactcga gctgcttgga gagttttgtc
     2281 aagagtcagg aaggagaaaa tgaagaagga agtgaggggg agctggtggt gaagtttggt
     2341 gagactcttc caaagctgaa gcccattatc tctgaccctg agtacctgct agaccagcac
     2401 atcctcatca gcatcaagtc ctctgacagc gacgaatcct atggcgaggg ctgcattgcc
     2461 cttcggttag aggccacaga aacgcagctg cccatctaca cgcctctcac ccaccatggg
     2521 gagttgacag gccacttcca gggggagatc aagctgcaga cctctcaggg caagacgagg
     2581 gagaagctct atgactttgt gaagacggag cgtgatgaat ccagtgggcc aaagaccctg
     2641 aagagcctca ccagccacga ccccatgaag cagtgggaag tcactagcag ggcccctccg
     2701 tgcagtggct ccagcatcac tgaaatcatc aaccccaact acatgggagt ggggcccttt
     2761 gggccaccaa tgcccctgca cgtgaagcag accttgtccc ctgaccagca gcccacagcc
     2821 tggagctacg accagccgcc caaggactcc ccgctggggc cctgcagggg agaaagtcct
     2881 ccgacacctc ccggccagcc gcccatatca cccaagaagt ttttaccctc aacagcaaac
     2941 cggggtctcc ctcccaggac acaggagtca aggcccagtg acctggggaa gaacgcaggg
     3001 gacacgctgc ctcaggagga cctgccgctg acgaagcccg agatgtttga gaaccccctg
     3061 tatgggtccc tgagttcctt ccctaagcct gctcccagga aggaccagga atcccccaaa
     3121 atgccgcgga aggaaccccc gccctgcccg gaacccggca tcttgtcgcc cagcatcgtg
     3181 ctcaccaaag cccaggaggc tgatcgcggc gaggggcccg gcaagcaggt gcccgcgccc
     3241 cggctgcgct ccttcacgtg ctcatcctct gccgagggca gggcggccgg cggggacaag
     3301 agccaaggga agcccaagac cccggtcagc tcccaggccc cggtgccggc caagaggccc
     3361 atcaagcctt ccagatcgga aatcaaccag cagaccccgc ccaccccgac gccgcggccg
     3421 ccgctgccag tcaagagccc ggcggtgctg cacctccagc actccaaggg ccgcgactac
     3481 cgcgacaaca ccgagctccc gcatcacggc aagcaccggc cggaggaggg gccaccaggg
     3541 cctctaggca ggactgccat gcag
//
