LOCUS       sequence-314222-        5061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector containing PIK3C2A tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, 
            McGarry L, Shanks E, Mostov KE, Bryant DM
  TITLE     The phospholipid PI(3,4)P2 is an apical identity determinant.
  JOURNAL   Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8.
  PUBMED    30487552
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-11-28"; volume: "9(1):5041. doi"; pages: " 
            10.1038/s41467-018-07464-8"
COMMENT     Sequence Label: pLEX303 GFP-PIK3C2A
FEATURES             Location/Qualifiers
     source          1..5061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggctcaga tatttagcaa cagcggattt aaagaatgtc catcttcaca tccggaacca
       61 acaagagcaa aagatgtgga caaagaagaa gcattacaga tggaagcaga ggctttagca
      121 aaactgcaaa aggatagaca agtgactgac aatcagagag gctttgagtt gtcaagcagc
      181 accagaaaaa aagcacaggt ttataacaag caggattatg atctcatggt gtttcctgaa
      241 tcagattccc aaaaaagagc attagatatt gatgtagaaa agctcaccca agctgaactt
      301 gagaaactat tgctggatga cagtttcgag actaaaaaaa cacctgtatt accagttact
      361 cctattctga gcccttcctt ttcagcacag ctctatttta gacctactat tcagagagga
      421 cagtggccac ctggattacc tgggccttcc acttatgctt taccttctat ttatccttct
      481 acttacagta aacaggctgc attccaaaat ggcttcaatc caagaatgcc cacttttcca
      541 tctacagaac ctatatattt aagtcttccg ggacaatctc catatttctc atatcctttg
      601 acacctgcca caccctttca tccacaagga agcttaccta tctatcgtcc agtagtcagt
      661 actgacatgg caaaactatt tgacaaaata gctagtacat cagaattttt aaaaaatggg
      721 aaagcaagga ctgatttgga gataacagat tcaaaagtca gcaatctaca ggtatctcca
      781 aagtctgagg atatcagtaa atttgactgg ttagacttgg atcctctaag taagcctaag
      841 gtggataatg tggaggtatt agaccatgag gaagagaaaa atgtttcaag tttgctagca
      901 aaggatcctt gggatgctgt tcttcttgaa gagagatcga cagcaaattg tcatcttgaa
      961 agaaaggtga atggaaaatc cctttctgtg gcaactgtta caagaagcca gtctttaaat
     1021 attcgaacaa ctcagcttgc aaaagcccag ggccatatat ctcagaaaga cccaaatggg
     1081 accagtagtt tgccaactgg aagttctctt cttcaagaag ttgaagtaca gaatgaggag
     1141 atggcagctt tttgtcgatc cattacaaaa ttgaagacca aatttccata taccaatcac
     1201 cgcacaaacc caggctattt gttaagtcca gtcacagcgc aaagaaacat atgcggagaa
     1261 aatgctagtg tgaaggtctc cattgacatt gaaggatttc agctaccagt tacttttacg
     1321 tgtgatgtga gttctactgt agaaatcatt ataatgcaag ccctttgctg ggtacatgat
     1381 gacttgaatc aagtagatgt tggcagctat gttctaaaag tttgtggtca agaggaagtg
     1441 ctgcagaata atcattgcct tggaagtcat gagcatattc aaaactgtcg aaaatgggac
     1501 acagaaatta gactacaact cttgaccttc agtgcaatgt gtcaaaatct ggcccgaaca
     1561 gcagaagatg atgaaacacc cgtggattta aacaaacacc tgtatcaaat agaaaaacct
     1621 tgcaaagaag ccatgacgag acaccctgtt gaagaactct tagattctta tcacaaccaa
     1681 gtagaactgg ctcttcaaat tgaaaaccaa caccgagcag tagatcaagt aattaaagct
     1741 gtaagaaaaa tctgtagtgc tttagatggt gtcgagactc ttgccattac agaatcagta
     1801 aagaagctaa agagagcagt taatcttcca aggagtaaaa ctgctgatgt gacttctttg
     1861 tttggaggag aagacactag caggagttca actaggggct cacttaatcc tgaaaatcct
     1921 gttcaagtaa gcataaacca attaactgca gcaatttatg atcttctcag actccatgca
     1981 aattctggta ggagtcctac agactgtgcc caaagtagca agagtgtcaa ggaagcatgg
     2041 actacaacag agcagctcca gtttactatt tttgctgctc atggaatttc aagtaattgg
     2101 gtatcaaatt atgaaaaata ctacttgata tgttcactgt ctcacaatgg aaaggatctt
     2161 tttaaaccta ttcaatcaaa gaaggttggc acttacaaga atttcttcta tcttattaaa
     2221 tgggatgaac taatcatttt tcctatccag atatcacaat tgccattaga atcagttctt
     2281 caccttactc tttttggaat tttaaatcag agcagtggaa gttcccctga ttctaataag
     2341 cagagaaagg gaccagaagc tttgggcaaa gtttctttac ctctttttga ctttaaacgg
     2401 tttttaacat gtggaactaa acttctatat ctttggactt catcacatac aaattctgtt
     2461 cctggaacag ttaccaaaaa aggatatgtc atggaaagaa tagtgctaca ggttgatttt
     2521 ccttctcctg catttgatat tatttataca actcctcaag ttgacagaag cattatacag
     2581 caacataact tagaaacact agagaatgat ataaaaggga aacttcttga tattcttcat
     2641 aaagactcat cacttggact ttctaaagaa gataaagctt ttttatggga gaaacgttat
     2701 tattgcttca aacacccaaa ttgtcttcct aaaatattag caagcgcccc aaactggaaa
     2761 tgggttaatc ttgccaaaac ttactcattg cttcaccagt ggcctgcatt gtacccacta
     2821 attgcattgg aacttcttga ttcaaaattt gctgatcagg aagtaagatc cctagctgtg
     2881 acctggattg aggccattag tgatgatgag ctaacagatc ttcttccaca gtttgtacaa
     2941 gctttgaaat atgaaattta cttgaatagt tcattagtgc aattcctttt gtccagggca
     3001 ttgggaaata tccagatagc acacaattta tattggcttc tcaaagatgc cctgcatgat
     3061 gtacagttta gtacccgata cgaacatgtt ttgggtgctc tcctgtcagt aggaggaaaa
     3121 cgacttagag aagaacttct aaaacagacg aaacttgtac agcttttagg aggagtagca
     3181 gaaaaagtaa ggcaggctag tggatcagcc agacaggttg ttctccaaag aagtatggaa
     3241 cgagtacagt ccttttttca gaaaaataaa tgccgtctcc ctctcaagcc aagtctagtg
     3301 gcaaaagaat taaatattaa gtcgtgttcc ttcttcagtt ctaatgctgt ccccctaaaa
     3361 gtcacaatgg tgaatgctga ccctatggga gaagaaatta atgtcatgtt taaggttggt
     3421 gaagatcttc ggcaagatat gttagcttta cagatgataa agattatgga taagatctgg
     3481 cttaaagaag gactagatct gaggatggta attttcaaat gtctctcaac tggcagagat
     3541 cgaggcatgg tggagctggt tcctgcttcc gataccctca ggaaaatcca agtggaatat
     3601 ggtgtgacag gatcctttaa agataaacca cttgcagagt ggctaaggaa atacaatccc
     3661 tctgaagaag aatatgaaaa ggcttcagag aactttatct attcctgtgc tggatgctgt
     3721 gtagccacct atgttttagg catctgtgat cgacacaatg acaatataat gcttcgaagc
     3781 acgggacaca tgtttcacat tgactttgga aagtttttgg gacatgcaca gatgtttggc
     3841 agcttcaaaa gggatcgggc tccttttgtg ctgacctctg atatggcata tgtcattaat
     3901 gggggtgaaa agcccaccat tcgttttcag ttgtttgtgg acctctgctg tcaggcctac
     3961 aacttgataa gaaagcagac aaaccttttt cttaacctcc tttcactgat gattccttca
     4021 gggttaccag aacttacaag tattcaagat ttgaaatacg ttagagatgc acttcaaccc
     4081 caaactacag acgcagaagc tacaattttc tttactaggc ttattgaatc aagtttggga
     4141 agcattgcca caaagtttaa cttcttcatt cacaaccttg ctcagcttcg tttttctggt
     4201 cttccttcta atgatgagcc catcctttca ttttcaccta aaacatactc ctttagacaa
     4261 gatggtcgaa tcaaggaagt ctctgttttt acatatcata agaaatacaa cccagataaa
     4321 cattatattt atgtagtccg aattttgagg gaaggacaga ttgaaccatc atttgtcttc
     4381 cgaacatttg acgaatttca ggaacttcac aataagctca gtattatttt tccactttgg
     4441 aagttaccag gctttcctaa taggatggtt ctaggaagaa cacacataaa agatgtagca
     4501 gccaaaagga aaattgagtt aaacagttac ttacagagtt tgatgaatgc ttcaacggat
     4561 gtagcagagt gtgatcttgt ttgtactttc ttccaccctt tacttcgtga tgagaaagct
     4621 gaagggatag ctaggtctgc agatgcaggt tccttcagtc ctactccagg ccaaatagga
     4681 ggagctgtga aattatccat ctcttaccga aatggtactc ttttcatcat ggtgatgcat
     4741 atcaaagatc ttgttactga agatggagct gacccaaatc catatgtcaa aacataccta
     4801 cttccagata accacaaaac atccaaacgt aaaaccaaaa tttcacgaaa aacgaggaat
     4861 ccgacattca atgaaatgct tgtatacagt ggatatagca aagaaaccct aagacagcga
     4921 gaacttcaac taagtgtact cagtgcagaa tctctgcggg agaatttttt cttgggtgga
     4981 gtaaccctgc ctttgaaaga tttcaacttg agcaaagaga cggttaaatg gtatcagctg
     5041 actgcggcaa catacttgta a
//
