LOCUS       sequence-31424-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BRPF1 (3L42)
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             515..532
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(585..603)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      599..646
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(770..789)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aattccctct gaatattttg tttactttaa gaaggagata taccatgcat catcatcatc
       61 atcacagcag cggcagagaa aacttgtatt tccagggcga ggactccccg ctggatgctc
      121 tggacctcgt gtgggccaaa tgccgaggct atccatcata cccagctctg atcattgatc
      181 caaagatgcc ccgagaaggt atgttccacc atggggttcc catccctgtg cccccactgg
      241 aggtgctgaa acttggggag cagatgaccc aggaagcccg agagcatctc tacctcgtcc
      301 tcttctttga caacaaacga acctggcagt ggctgcccag gaccaagctg gttcctctgg
      361 gtgtgaacca ggacctagac aaggagaaga tgctggaggg ccgcaagtcc aacatccgca
      421 agtcagtaca gatcgcctac cacagggctc tgcagcaccg cagcaaggtg caaggcgagc
      481 agagctgatg acgaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc
      541 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      601 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      661 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      721 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      781 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
      841 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
      901 ggtgat
//
