LOCUS       sequence-31425-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CENTA1 (3LJU)
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg
       61 cagagaaaac ttgtatttcc agggcaagga gcggcgcagg gcggtcctgg agctgctgca
      121 gcggccgggg aacgcgcgct gcgcggactg cggcgccccg gatcccgact gggcctccta
      181 cactctgggc gtcttcatct gcctgagctg ctcgggaatc caccggaata tcccccaggt
      241 cagcaaggtg aagtccgtcc gcctggacgc ctgggaggag gcccaagtgg agttcatggc
      301 ctcccacggg aacgacgccg cgagagccag gtttgagtcc aaagtaccct ccttctacta
      361 ccggcccacg ccctccgact gccagctcct tcgagagcag tggatccggg ccaagtacga
      421 gcgacaggag ttcatctacc cggagaagca ggagccctac tcggcagggt accgtgaggg
      481 ttttctctgg aagcgtggcc gggacaacgg gcagtttttg agccggaagt ttgtgctgac
      541 agaacgagag ggtgctctga agtatttcaa cagaaatgat gccaaggagc ccaaggccgt
      601 gatgaagatc gagcacctga acgccacctt ccagccggcc aagatcggcc acccccacgg
      661 cctgcaggtc acctacctga aggacaacag cacccgtaac atcttcatct accatgagga
      721 cgggaaggag attgtggact ggttcaatgc actccgagct gctcgcttcc actacctgca
      781 ggtggcat
//
