LOCUS       sequence-31426-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FXR1
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             518..535
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(588..606)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      602..649
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(773..792)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tccctctgaa tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc
       61 acagcagcgg cagagaaaac ttgtatttcc agggcgcgga gctgacggtg gaggttcgcg
      121 gctctaacgg ggctttctac aagggattta tcaaagatgt tcatgaagac tcccttacag
      181 ttgtttttga aaataattgg caaccagaac gccaggttcc atttaatgaa gttagattac
      241 caccaccacc tgatataaaa aaagaaatta gtgaaggaga tgaagtagag gtatattcaa
      301 gagcaaatga ccaagagcca tgtgggtggt ggttggctaa agttcggatg atgaaaggag
      361 aattttatgt cattgaatat gctgcttgtg acgctactta caatgaaata gtcacatttg
      421 aacgacttcg gcctgtcaat caaaataaaa ctgtcaaaaa aaataccttc tttaaatgca
      481 cagtggattg atgacgaagc ttgcggccgc actcgagcac caccaccacc accactgaga
      541 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      601 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      661 aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
      721 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      781 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatc
//
