LOCUS       sequence-31427-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BRD1
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             490..507
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(560..578)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      574..621
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(745..764)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg
       61 cagagaaaac ttgtatttcc agggctcggt gctggagcct ctgaaggtgg tgtgggccaa
      121 gtgcagcggc tacccctcct acccggcact gatcatcgac cccaagatgc cccgtgtgcc
      181 tggccaccac aacggcgtca ccatcccggc cccacccctg gacgtgctga agattgggga
      241 gcacatgcag accaagtctg atgagaagct gttcctcgtt ctcttttttg ataataagag
      301 aagttggcag tggcttccta agtccaaaat ggttcccctt ggtattgacg aaactataga
      361 caagttaaag atgatggaag ggaggaattc cagcatccgg aaggccgtgc ggatcgcttt
      421 tgaccgcgcc atgaaccacc tgagccgcgt ccacggggag tgatgacgaa gcttgcggcc
      481 gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa
      541 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      601 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg
      661 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      721 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      781 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
      841 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agt
//
