LOCUS       sequence-314275-       13904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  10-unit intMEMOIR recording array for mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13904)
  AUTHORS   Chow KK, Budde MW, Granados AA, Cabrera M, Yoon S, Cho S, Huang TH, 
            Koulena N, Frieda KL, Cai L, Lois C, Elowitz MB
  TITLE     Imaging cell lineage with a synthetic digital recording system.
  JOURNAL   Science. 2021 Apr 9;372(6538). pii: 372/6538/eabb3099. doi: 
            10.1126/science.abb3099.
  PUBMED    33833095
REFERENCE   2  (bases 1 to 13904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2021-04-9"; volume: "372(6538). pii: 372/6538/eabb3099. doi";
            pages: " 10.1126/science.abb3099"
COMMENT     Sequence Label: R26-pCAG-Ceru-10unit-BGHpA
FEATURES             Location/Qualifiers
     source          1..13904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..795
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1..795
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(55..74)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     665..684
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    929..1050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(966..985)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1020..1039
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        1146..1449
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1451..1728
                     /label=chicken beta-actin promoter
     intron          1729..2745
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2669..2691
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2753..2772
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2859..3236
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      3255..3264
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3261..3263,3264..3266,3267..3980)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3306..3327)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3567..3586)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3914..3935
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4001..4038
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    4555..4602
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    5261..5298
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    5815..5862
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     RBS             7372..7380
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    8534..8581
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    8594..8641
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    9158..9195
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    10423..10460
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     polyA_signal    10481..10705
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    10726..10774
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(11445..12033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11525..11544)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12204..12995,12996..13064))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12827..12846
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13065..13156)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13164..13181
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     misc_feature    join(13735..13800,13801..13838)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     promoter        13839..13886
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
       61 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
      121 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
      181 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
      241 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
      301 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
      361 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
      421 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
      481 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
      541 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
      601 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
      661 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
      721 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
      781 gacgagttct tctgatgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
      841 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      901 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      961 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1021 tggtttgtcc aaactcatca atgtatctta ggtctcgcgt actgtcgtaa attctgcagg
     1081 acttctagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     1141 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     1201 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     1261 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     1321 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     1381 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     1441 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
     1501 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
     1561 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
     1621 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     1681 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
     1741 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     1801 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     1861 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
     1921 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
     1981 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
     2041 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
     2101 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
     2161 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
     2221 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
     2281 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     2341 gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc
     2401 gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct
     2461 ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg
     2521 cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
     2581 tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc
     2641 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct
     2701 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
     2761 ctggttattg tgctgtctca tcattttggc aaagaattga tttgataccg cgggcccggg
     2821 atcccctcga gggaattacc tcagacgtag ccgccaccat gccagagcca gcgaagtctg
     2881 ctcccgcccc gaaaaagggc tccaagaagg cggtgactaa ggcgcagaag aaaggcggca
     2941 agaagcgcaa gcgcagccgc aaggagagct attccatcta tgtgtacaag gttctgaagc
     3001 aggtccaccc tgacaccggc atttcgtcca aggccatggg catcatgaat tcgtttgtga
     3061 acgacatttt cgagcgcatc gctggtgagg cttcccgcct ggcgcattac aacaagcgct
     3121 cgaccatcac ctccagggag atccagacgg ccgtgcgcct gctgctgcct ggggagttgg
     3181 ccaagcacgc cgtgtccgag ggtactaagg ccatcaccaa gtacaccagc gctaaggatc
     3241 cccgggtacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     3301 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     3361 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     3421 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc
     3481 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     3541 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     3601 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     3661 acggcaacat cctggggcac aagctggagt acaacgccat cagcgacaac gtctatatca
     3721 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
     3781 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     3841 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
     3901 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     3961 tggacgagct gtacaagtga acctgtagcg tactgtcgta ggcttgtcga cgacggcgat
     4021 ctccgtcgtc aggatcatac ctgtagtctt aaggtccttt atgaggcttg cccacgtgct
     4081 caagtccatc aagaaaggga cctcgcacta ggcccactaa ttactgatgc ttaagttcac
     4141 ctcatcttga gctgctctaa tgcccagatt tgagatgaag ctcagtggat agtgccaact
     4201 ccactggcca acctaaccta gcttggaggt ggcaaaagat aaggttggct gtttagcctc
     4261 tgtactccac ctcagcgagc tctggatgac catttactgg gctaccgaca ctttaggagg
     4321 gacctaaata aggtctgtga tgtttcactt tggtgggagg accagcagtg cccaactggc
     4381 taccttcctc aaatcacact gtggtcatgc atcctttggc caccttaatt gggagatgca
     4441 ctactcaaca accgtcctac tcctaatagg agtggggtgt gacaagttta gactgcctct
     4501 aacctcttgg tttcaattga ggggcacatc taaagtgcag ttcttggaac cgtaggtttg
     4561 tctggtcaac caccgcgatc tcagtggtgt acggtacaaa ccacctagac acctggttcg
     4621 tactgtcgta ggcttgtcga cgacggcgcg ctccgtcgtc aggatcatac ctgtagtatt
     4681 caactatgac tgatggggtc ccaaactcct tatgctttcc catccaagca tatggagggg
     4741 agcagatagt aatacttcct acgcagttgg ccctatggaa gcctaaaact atgtcttagc
     4801 tgcctacagc tcttatgacc cactggtaga tgagggcatc actgacgtga cctaggtgtg
     4861 ttataaggat ttccctagag tagggtctga ctgctaatgc agtgagagtg gtgttcccac
     4921 atgccacatc cttatagggt ggcagcagct cttgtgtgat gtacacttgg cacttaaagt
     4981 gctatggaca atggcacctc aaggacagct tgcgacccac aacattctaa ccactgggca
     5041 cccagtgcca aagagcaatg gggctgacat agcagacttt agctcaacta gcggatgtgg
     5101 cctagtaaca ctatggctcc atgggataac aagaatgtat ccctagaggc cctaaatcac
     5161 cataccacgc cctcttgaac cgtaggtttg tctggtcaac caccgcgcgc tcagtggtgt
     5221 acggtacaaa ccacctagac acctttggcg tactgtcgta ggcttgtcga cgacggcggc
     5281 ctccgtcgtc aggatcatac ctgtaggaat tacaacagtt gagctgtgta gcactgggaa
     5341 tatagggaga caagaggtgg cccctccctc tgaggataac agttggtatg catgtttgat
     5401 ctgggtgtcc tacatgggct catagagtct ccaatgaccc caacgtgtat gctgaaggga
     5461 gcagtatatg ttggaagcgg tattcgtgag gtaggtgttg tggacgaaac ccttgtgcca
     5521 gaggtagttc agtctatgca ttgtcagcca tcatggtgat gggtgtagca catagtctaa
     5581 gctgttaagt atctctgctg agaggaggaa gtggctagat tgtgtgatga ccttttgggc
     5641 ctcaatcatg tgtagctggg ttaagcagtg acaagctgac aattgattgg aacaccttta
     5701 gctcttccac ctagctactc tggaggctca gctctctgta attgctcccc tttccacaga
     5761 tattgctgaa ggggttatac aaggatcatt gagggccaat gggtgcgaac cgtaggtttg
     5821 tctggtcaac caccgcggcc tcagtggtgt acggtacaaa ccacctagac acctgagtcg
     5881 tactgtcgta ggcttgtcga cgacggcgta ctccgtcgtc aggatcatac ctgtaggtac
     5941 cttcaaggag tagggaaggc attatttggg accctaaagc cccatgaacc attgtacttt
     6001 gttcagaaat tggagctcaa acaatgagct ctggcctcag tggcctacat gctgacgtgg
     6061 tggccgaccc agatgtgggg attaactaat gggcggctat ttcacatgta ggacgcctaa
     6121 tcaaggctat gaacacatgg tgtcaaactc ccaatgaaac agcagtgcca cagctggtct
     6181 gctcatcagt agacatggtg aagtgcctta tgaagggtgc aaggagttgt gtcagaatgg
     6241 aactgctctc tctatgtttg gactcagaat ggagtaatcc acagttgcta tgtggggtgg
     6301 aacattccca agagatcttg ccaacatcca cctttagcac atgaggagag tcccatctgg
     6361 gcctttgagc ttgaggcaga ggttgtttgc agagtctgga ctacttgatt tggaaggtac
     6421 ttacactagt tcggttgaac cgtaggtttg tctggtcaac caccgcgtac tcagtggtgt
     6481 acggtacaaa ccacctagac acctgctacg tactgtcgta ggtttgtctg gtcaaccacc
     6541 gcgacctcag tggtgtacgg tacaaaccac ctcagaaggt ggtttgtacc gtacaccact
     6601 gaggtcgcgg tggttgacca gacaaaccta cggtagatta tccctagaaa atgagagttt
     6661 ggatgtagtg tcctagtgag actggccctc attttgttgt ggtggtctta ggctgggaca
     6721 acaccctagt gtttgtccct cgagcaaagc cctctatcca tgatgtactg cactatagca
     6781 caccttccta gttactgggg atgcttgtaa tccaccagct cccgactact tttagggaag
     6841 agcactagtg ggcctacctc tttggagatg taccaccacg tcttttgcaa gagccccaga
     6901 ctggcctttg tttctatggt cgggatggaa ctgcggcagt catattcttt gcttagacag
     6961 ccccttagct ctgcagctga aatttatcca gtggtgaatt cagagtgggc agaggctgcc
     7021 agatgctccc taggtagctt gggcaagtat tgcttctgtg tgcaatctgt taatggtcag
     7081 agagttctgc agctataatg tgcttgtgaa ctcaatctcc ctctggagag catcccgaac
     7141 cgtaggcttg tcgacgacgg cgacctccgt cgtcaggatc atacctagac acctcagacg
     7201 tactgtcgta ggtttgtctg gtcaaccacc gcgcactcag tggtggttga ccagacaaac
     7261 ctacggtagg gacttggtgt gctacattgg tgaatgggtg cacacaagct cttgggctga
     7321 ggtttagact aatctgtcta gttctgggca cagtgtatgt atcattggac cacctcctta
     7381 gtggactggt caatatgagc cattcatagc tagcgctgcc ccatgacact ggctgcagct
     7441 taacgggctc tctaagaagg gtgaaccaaa atttcagaac tgagagagtt ggggaggagg
     7501 ttaattagag tgaggcagta gttggccaca catggcagcg tagcaagcag attgaggaat
     7561 ttggaatggt tgctgtaagg acctaaaagg tgtgcactaa gctagtgcca tgacagttgc
     7621 agagctagct agttatggga ccagcctcaa ttctggagag cactagggtt tgaaaagaac
     7681 tgttatcatg cagacgctat taggatgagg gccacggtgg ctgatagagg atagttaatc
     7741 tcccctctgc catttagtcc agtccacatg aaccgtaggc ttgtcgacga cggcgcactc
     7801 cgtcgtcagg atcataccta gacacctgta gcgtactgtc gtaggtttgt ctggtcaacc
     7861 accgcgccct cagtggtgta cggtacaaac cacctcagaa ggtggtttgt accgtacacc
     7921 actgagggcg cggtggttga ccagacaaac ctacggtagg gataatgtcc ccagtggcct
     7981 ctttcacact tctgaactag aactatgcag agtagcggga cttgtggaga agccatgggt
     8041 ggtgtttact ctttggggcc atcaagacat ggtctggtgc catgtgagaa aggcagcctt
     8101 tacagagttc acttatgccc ctatggcaat tgggaggtag ggctggttga ctttgcttgg
     8161 gccttgtcta ctagggaacc tcacaaggtg actatacgcc actggcagca tataaagagg
     8221 tgggccgagt acgctgagct gctgaattgc tggaatgaca ctagtggcat tgccacatat
     8281 tccaagccag agcagagagg aaatgtgtgg tatgaatcta ctaagttgct gatctgggtg
     8341 actgacctag tctccttggg gactagcaaa agatgcaccc actgctagcc tctttaagag
     8401 aatgcggtac tacaaacaat atggctgagt caccattcac tctgtcagca agtgggcttg
     8461 aaccgtaggc ttgtcgacga cggcgccctc cgtcgtcagg atcataccta gacacctggt
     8521 tcgtactgtc gtaggtttgt ctggtcaacc accgcgctct cagtggtgta cggtacaaac
     8581 cacctcagaa ggtggtttgt accgtacacc actgagagcg cggtggttga ccagacaaac
     8641 ctacggtagt gtcagacaag ggaagtccca gagggtcggg ttgggctagt gacaaacctc
     8701 tgatcctttg gccccacagc catctcccat gcatagctag tttgagtaaa gtcacgaata
     8761 agcacttgtc accccttact ggtaggctgg ttgatagctt gacctaccat tggggcccta
     8821 gaatctctga aaggtctagg gctccatggt ttgagtactc ttggaggtct gctataaaac
     8881 aatcgacgtc aagcaacacc ccagcctaat tcctataggg gcagtctctc cgttgagtag
     8941 taactggggt gctctgatag ctaactgtag tggtgtcctc cctttccaag aaaaggtatg
     9001 agttgtccct tggctttggg ttattcccct aattggcaca ttctatgtct gggagggcct
     9061 aggtagggaa gattgcccaa tgcagagtat ggcaaatggg aacatagagt ccacattgac
     9121 ctatgtttct accacttaac cagatgcccg aaccgtaggc ttgtcgacga cggcgctctc
     9181 cgtcgtcagg atcataccta gacacctttg gcgtactgtc gtaggtttgt ctggtcaacc
     9241 accgcgtcct cagtggtgta cggtacaaac cacctcagaa ggtggtttgt accgtacacc
     9301 actgaggacg cggtggttga ccagacaaac ctacggtagc atgtcaaagc ctctgtgagc
     9361 agaagcctta aggcgggctc cccatagagg gtgcaacatc cagcttatgg acagaatatg
     9421 ctcactatta catgtaggcc ccatcgcaag tcagcccaga tgcatgatat gcattcagca
     9481 atagcatgga ggcttgtcct taggtgccca atggtgggga cggtgtccat gagtagaatc
     9541 cagtgcaagg ttgaactgca tgcaatggat agcaaaaggc atccccaagg gaggctatcg
     9601 gggttaaggg cactcactct atagttacta gacactgtga gatggatggt gatctgtctg
     9661 ctgtatctgg ataagtacct ctgcaatcgc aagtggaggg ttggaggatg ttgtgaggtc
     9721 caagatggaa gggtcaaata ggcatattgg tcacagcact taaccttgtg ctgtcatcag
     9781 atggtgcagt gatatcagtc tatgtgggaa cagcatactg gctcagctac ctccttctcg
     9841 aaccgtaggc ttgtcgacga cggcgtcctc cgtcgtcagg atcataccta gacacctgag
     9901 tcgtactgtc gtaggtgtgt aggactacac agcttttggg aaagattgga ggggaagctt
     9961 caagttatgg atcttatctt ccgctcttag aactctacac tgatgtaggt gggccctaga
    10021 gaagcagttg catgcctcta agggctgata tggcaaatgc ctatgggcag caacagagtc
    10081 acatgcttcc tcctagtaag atcctttagg caccaaggca atagcagtga gcacatctgg
    10141 tgattagcac atccaaggca tcctacttgt gggactgacc tactcatgag atgggatggg
    10201 gaaccagttc tcaaatgggc ccttctgcaa gtgctatctt gtgctgccac ttggatgaga
    10261 tcacagatgg gctgcaagga acctctagtc aaatgcggcc attcatgctg catgcgaggt
    10321 caatttctac aacctactaa atttgaggct cgggcttccg tggcaaccta caagtcactg
    10381 agctgagtgg gtcattccta gaagcctcag tatggaaccg taggcttgtc gacgacggcg
    10441 ttctccgtcg tcaggatcat acctagacac ctgaacaggt ctgtgccttc tagttgccag
    10501 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
    10561 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
    10621 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagagaa tagcaggcat
    10681 gctggggatg cggtgggctc tatggtacga gacacctcga gacccaataa aagatcttta
    10741 ttttcattag atctgtgtgt tggttttttg tgtgtctaga gtgtgggtgt gggcgttgtc
    10801 ctgcagggga attgaacagg tgtaaaattg gagggacaag acttcccaca gattttcggt
    10861 tttgtcggga agttttttaa taggggcaaa taaggaaaat gggaggatag gtagtcatct
    10921 ggggttttat gcagcaaaac tacaggttat tattgcttgt gatccgcctc ggagtatttt
    10981 ccatcgaggt agattaaaga catgctcacc cgagttttat actctcctgc ttgagatcct
    11041 tactacagta tgaaattaca gtgtcgcgag ttagactatg taagcagaat tttaatcatt
    11101 tttaaagagc ccagtacttc atatccattt ctcccgctcc ttctgcagcc ttatcaaaag
    11161 gtattttaga acactcattt tagccccatt ttcatttatt atactggctt atccaacccc
    11221 tagacagagc attggcattt tccctttcct gatcttagaa gtctgatgac tcatgaaacc
    11281 agacagatta ccctgttatc cctagaattc agcttgggat aaaaagctat ggcataggcg
    11341 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    11401 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    11461 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    11521 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    11581 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    11641 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    11701 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    11761 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    11821 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    11881 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    11941 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    12001 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    12061 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    12121 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    12181 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    12241 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    12301 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagatcc acgctcaccg
    12361 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    12421 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    12481 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    12541 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    12601 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    12661 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    12721 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    12781 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    12841 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    12901 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    12961 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    13021 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    13081 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    13141 tagaaaaata aacaaatagg ggtgatttaa tctgtatcag gggcgtatag tggagcaaag
    13201 cgaattctaa ctataacggt cctaaggtag cgaaggccct cccctcggcc ccgcgccgca
    13261 gagtctggcc gcgcgcccct gcgcaacgtg gcaggaagcg cgcgctgggg gcggggacgg
    13321 gcagtagggc tgagcggctg cggggcgggt gcaagcacgt ttccgacttg agttgcctca
    13381 agaggggcgt gctgagccag acctccatcg cgcactccgg ggagtggagg gaaggagcga
    13441 gggctcagtt gggctgtttt ggaggcagga agcacttgct ctcccaaagt cgctctgagt
    13501 tgttatcagt aagggagctg cagtggagta ggcggggaga aggccgcacc cttctccgga
    13561 ggggggaggg gagtgttgca atacctttct gggagttctc tgctgcctcc tggcttctga
    13621 ggaccgccct gggcctggga gaatcccttc cccctcttcc ctcgtgatct gcaactccag
    13681 tctttctaga agatgggcgg gagtcttttg ggcaggctta aaggctaacc tggttagggc
    13741 gcagtagtcc agggtttcct tgatgatgtc atacttatcc tgtccctttt ttttccacag
    13801 ctcgcggttg aggacaaact cttcgcggtc tttccagtgt tgacaattaa tcatcggcat
    13861 agtatatcgg catagtataa tacgacaagg tgaggaacgc cacc
//
