LOCUS       sequence-314282-        8457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses eGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Gstalder C, Liu D, Miao D, Lutterbach B, DeVine AL, Lin C, Shettigar
            M, Pancholi P, Buchbinder EI, Carter SL, Manos MP, Rojas-Rudilla V, 
            Brennick R, Gjini E, Chen PH, Lako A, Rodig S, Yoon CH, Freeman GJ, 
            Barbie DA, Hodi FS, Miles W, Van Allen EM, Haq R
  TITLE     Inactivation of Fbxw7 impairs dsRNA sensing and confers resistance 
            to PD-1 blockade.
  JOURNAL   Cancer Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416.
  PUBMED    32371478
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416."
COMMENT     Sequence Label: pLX304_zeo_eGFP
FEATURES             Location/Qualifiers
     source          1..8457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..501
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     420..438
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             513..887
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    947..1064
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1181..1484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1485..1688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1639..1659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1711..1735
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1756..1758,1759..1761,1762..2472)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1801..1822)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2062..2081)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2409..2430
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2492..2516)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2518..2559
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2601..3189
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2654..2674)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3072..3083)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3261..3494
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3572..3693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3609..3628)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3663..3682
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3733..3868
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3888..3907)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3889..3907)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3917..3934)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3917..3933)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3926..3948)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4075..4530
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4162..4181)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4372..4393
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4556..4660
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4661..4729,4730..5521)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4879..4898)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5692..6280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6181..6200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6434..6451
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6568..6589
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6604..6609,6610..6627,6628..6634)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6642..6658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6647..6669
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6666..6682
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6666..6682
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6701..6721
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6703..6721
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6749..6975
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6976..7156
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7203..7328
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7821..8054
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8239..8283
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
       61 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      121 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      181 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      241 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      301 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
      361 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
      421 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
      481 atcaccgacc tctctcccca gggggtacca ccatggccaa gttgaccagt gccgttccgg
      541 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
      601 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
      661 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
      721 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
      781 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
      841 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgactg cagctgcagt
      901 agtaagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      961 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1021 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     1081 acagcagaga tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa
     1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     1501 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     1561 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     1621 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     1681 gcagagctct ctggctaact gtcgggatca acaagtttgt acaaaaaagc aggctccgcg
     1741 gccgccccct tcaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1801 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtctgg cgagggcgag
     1861 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1921 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     1981 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     2041 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     2101 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2161 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     2221 gacaagcaga agaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc
     2281 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     2341 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     2401 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     2461 gagctgtaca agtccaaggg tgggcgcgcc gacccagctt tcttgtacaa agtggttggt
     2521 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac
     2581 cggtggcgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     2641 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     2701 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     2761 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     2821 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     2881 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     2941 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     3001 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     3061 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3121 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     3181 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     3241 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     3301 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     3361 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     3421 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     3481 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
     3541 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     3601 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3661 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     3721 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3781 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3841 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     3901 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     3961 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     4021 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     4081 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4141 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4201 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4261 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4321 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4381 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4441 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4501 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     4561 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4621 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4681 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4741 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4801 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4861 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4921 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4981 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5041 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5101 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5161 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5221 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5281 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5341 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5401 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5461 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5521 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5581 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5641 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5701 tttttttctg cgcataatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5761 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5821 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5881 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5941 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6001 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6061 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6121 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6181 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6241 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6301 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6361 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     6421 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     6481 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     6541 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     6601 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     6661 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     6721 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     6781 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     6841 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     6901 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     6961 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7021 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7081 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7141 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     7201 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     7261 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     7321 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     7381 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     7441 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     7501 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     7561 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     7621 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     7681 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     7741 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     7801 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     7861 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     7921 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     7981 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     8041 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     8101 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     8161 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     8221 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     8281 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     8341 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     8401 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggaccc
//
