LOCUS       sequence-314284-        3811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine Irf1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Gstalder C, Liu D, Miao D, Lutterbach B, DeVine AL, Lin C, Shettigar
            M, Pancholi P, Buchbinder EI, Carter SL, Manos MP, Rojas-Rudilla V, 
            Brennick R, Gjini E, Chen PH, Lako A, Rodig S, Yoon CH, Freeman GJ, 
            Barbie DA, Hodi FS, Miles W, Van Allen EM, Haq R
  TITLE     Inactivation of Fbxw7 impairs dsRNA sensing and confers resistance 
            to PD-1 blockade.
  JOURNAL   Cancer Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416.
  PUBMED    32371478
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416."
COMMENT     Sequence Label: pDONR221_mmIrf1 (closed)
FEATURES             Location/Qualifiers
     source          1..3811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1156..1255)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1272..1291)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1273..1291)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1296..1312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1296..1312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1425..2234
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MNHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2381..2969
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2870..2889
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      3036..3491
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3123..3142)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3333..3354
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     terminator      complement(3543..3570)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3662..3748)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(3797..3811,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3811,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg cttgaaggaa ttcggtacca tgccaatcac tcgaatgcgg atgagaccct
      181 ggctagagat gcagattaat tccaaccaaa tcccagggct gatctggatc aataaagaag
      241 agatgatctt ccagattcca tggaagcacg ctgctaagca cggctgggac atcaacaagg
      301 atgcctgtct gttccggagc tgggccattc acacaggccg atacaaagca ggagaaaaag
      361 agccagatcc caagacatgg aaggcaaact tccgttgtgc catgaactcc ctgccagaca
      421 tcgaggaagt gaaggatcag agtaggaaca agggcagctc tgctgtgcgg gtgtaccgga
      481 tgctgccacc cctcaccagg aaccagagga aagagagaaa gtccaagtcc agccgagaca
      541 ctaagagcaa aaccaagagg aagctgtgtg gagatgttag cccggacact ttctctgatg
      601 gactcagcag ctctacccta cctgatgacc acagcagtta caccactcag ggctacctgg
      661 gtcaggactt ggatatggaa agggacataa ctccagcact gtcaccgtgt gtcgtcagca
      721 gcagtctctc tgagtggcat atgcagatgg acattatacc agatagcacc actgatctgt
      781 ataacctaca ggtgtcaccc atgccttcca cctccgaagc cgcaacagac gaggatgagg
      841 aagggaagat agccgaagac cttatgaagc tctttgaaca gtctgagtgg cagccgacac
      901 acatcgatgg caagggatac ttgctcaatg agccagggac ccagctctct tctgtctatg
      961 gagacttcag ctgcaaagag gaaccagaga ttgacagccc tcgaggggac attgggatag
     1021 gcatacaaca tgtcttcacg gagatgaaga atatggactc catcatgtgg atggacagcc
     1081 tgctgggcaa ctctgtgagg ctgccgccct ctattcaggc cattccttgt gcaccatagc
     1141 tcgagtgcgg ccgcaaccca gctttcttgt acaaagttgg cattataaga aagcattgct
     1201 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     1261 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     1321 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     1381 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgaac catattcaac
     1441 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     1501 gggcccgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     1561 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     1621 agatggtcag actaaactgg ctgacggaat ttatgcctct cccgaccatc aagcatttta
     1681 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
     1741 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     1801 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     1861 gactcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     1921 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     1981 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     2041 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     2101 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     2161 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     2221 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     2281 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     2341 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2401 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2461 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2521 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2581 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2641 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2701 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2761 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2821 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2881 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2941 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3001 tggccttttg ctggcctttt gctcacatgt atgggacgcg ccctgtagcg gcgcattaag
     3061 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3121 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3181 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3241 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3301 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3361 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     3421 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     3481 gtttacaatt tcacatgtgc tgaggatcga gtcttaatta ctgccggcct tgtagaaacg
     3541 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     3601 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     3661 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     3721 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     3781 ctagcatgga tgttttccca gtcacgacgt t
//
