LOCUS       sequence-314285-        8724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine Irf1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8724)
  AUTHORS   Gstalder C, Liu D, Miao D, Lutterbach B, DeVine AL, Lin C, Shettigar
            M, Pancholi P, Buchbinder EI, Carter SL, Manos MP, Rojas-Rudilla V, 
            Brennick R, Gjini E, Chen PH, Lako A, Rodig S, Yoon CH, Freeman GJ, 
            Barbie DA, Hodi FS, Miles W, Van Allen EM, Haq R
  TITLE     Inactivation of Fbxw7 impairs dsRNA sensing and confers resistance 
            to PD-1 blockade.
  JOURNAL   Cancer Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416.
  PUBMED    32371478
REFERENCE   2  (bases 1 to 8724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416."
COMMENT     Sequence Label: pLX304_zeo_mmIrf1
FEATURES             Location/Qualifiers
     source          1..8724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1579..1603)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1605..1646
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1688..2276
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1741..1761)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2159..2170)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2348..2581
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2659..2780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2696..2715)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2750..2769
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2820..2955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2975..2994)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2976..2994)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3004..3021)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3004..3020)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3013..3035)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3162..3617
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3249..3268)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3459..3480
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3643..3747
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3748..3816,3817..4608)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3966..3985)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4779..5367
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5268..5287
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5521..5538
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5655..5676
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5691..5696,5697..5714,5715..5721)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5729..5745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5734..5756
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5753..5769
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5753..5769
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5788..5808
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5790..5808
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5836..6062
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6063..6243
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6290..6415
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6908..7141
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7326..7370
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7545..8045
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7964..7982
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8057..8431
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    8491..8608
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagc aggcttgaag gaattcggta ccatgccaat cactcgaatg cggatgagac
      601 cctggctaga gatgcagatt aattccaacc aaatcccagg gctgatctgg atcaataaag
      661 aagagatgat cttccagatt ccatggaagc acgctgctaa gcacggctgg gacatcaaca
      721 aggatgcctg tctgttccgg agctgggcca ttcacacagg ccgatacaaa gcaggagaaa
      781 aagagccaga tcccaagaca tggaaggcaa acttccgttg tgccatgaac tccctgccag
      841 acatcgagga agtgaaggat cagagtagga acaagggcag ctctgctgtg cgggtgtacc
      901 ggatgctgcc acccctcacc aggaaccaga ggaaagagag aaagtccaag tccagccgag
      961 acactaagag caaaaccaag aggaagctgt gtggagatgt tagcccggac actttctctg
     1021 atggactcag cagctctacc ctacctgatg accacagcag ttacaccact cagggctacc
     1081 tgggtcagga cttggatatg gaaagggaca taactccagc actgtcaccg tgtgtcgtca
     1141 gcagcagtct ctctgagtgg catatgcaga tggacattat accagatagc accactgatc
     1201 tgtataacct acaggtgtca cccatgcctt ccacctccga agccgcaaca gacgaggatg
     1261 aggaagggaa gatagccgaa gaccttatga agctctttga acagtctgag tggcagccga
     1321 cacacatcga tggcaaggga tacttgctca atgagccagg gacccagctc tcttctgtct
     1381 atggagactt cagctgcaaa gaggaaccag agattgacag ccctcgaggg gacattggga
     1441 taggcataca acatgtcttc acggagatga agaatatgga ctccatcatg tggatggaca
     1501 gcctgctggg caactctgtg aggctgccgc cctctattca ggccattcct tgtgcaccat
     1561 agctcgagtg cggccgcaac ccagctttct tgtacaaagt ggttggtaag cctatcccta
     1621 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
     1681 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1741 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     1801 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1861 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     1921 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     1981 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2041 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2101 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2161 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2221 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     2281 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     2341 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     2401 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     2461 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     2521 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     2581 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     2641 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2701 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2761 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     2821 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     2881 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     2941 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     3001 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     3061 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     3121 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     3181 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3241 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3301 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3361 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3421 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3481 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3541 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     3601 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3661 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3721 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3781 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3841 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3901 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3961 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4021 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4081 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4141 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4201 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4261 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4321 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4381 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4441 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4501 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4561 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4621 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4681 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4741 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4801 ataatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4861 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4921 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4981 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5041 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5101 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5161 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5221 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5281 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5341 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5401 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5461 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5521 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     5581 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     5641 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     5701 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     5761 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     5821 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     5881 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     5941 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     6001 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     6061 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6121 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6181 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6241 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     6301 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     6361 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     6421 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     6481 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     6541 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     6601 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     6661 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     6721 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     6781 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     6841 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     6901 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     6961 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     7021 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     7081 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     7141 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     7201 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     7261 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     7321 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     7381 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     7441 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     7501 caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc cttttccaag
     7561 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
     7621 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
     7681 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
     7741 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
     7801 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
     7861 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
     7921 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
     7981 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
     8041 cccagggggt accaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     8101 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     8161 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     8221 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     8281 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     8341 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     8401 cgtgcacttc gtggccgagg agcaggactg actgcagctg cagtagtaag aattctagat
     8461 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     8521 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     8581 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     8641 ttggcgccgg ctcgaggggg cccggggtta ttaatagtaa tcaattacgg ggtcattagt
     8701 tcatagccca tatatggagt tccg
//
