LOCUS       sequence-314286-        5151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine Mavs in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Gstalder C, Liu D, Miao D, Lutterbach B, DeVine AL, Lin C, Shettigar
            M, Pancholi P, Buchbinder EI, Carter SL, Manos MP, Rojas-Rudilla V, 
            Brennick R, Gjini E, Chen PH, Lako A, Rodig S, Yoon CH, Freeman GJ, 
            Barbie DA, Hodi FS, Miles W, Van Allen EM, Haq R
  TITLE     Inactivation of Fbxw7 impairs dsRNA sensing and confers resistance 
            to PD-1 blockade.
  JOURNAL   Cancer Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416.
  PUBMED    32371478
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416."
COMMENT     Sequence Label: pENTR1A_Mavs (open)
FEATURES             Location/Qualifiers
     source          1..5151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             2002..2010
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             2364..2372
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(3023..3122)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3234..3253)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3245..4054
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3312..3331)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4144..4732
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4633..4652
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      4897..4983
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5075..5102
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     5110..5133
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ggtaccggtc cggaattccc
      121 gggatatcgt cgacccacgc gtccgcttgt ccatctcagt ccatcccacc ctgccagggt
      181 ccgagtcact ccagaagctg agcagccatg acatttgctg aggacaagac ctataagtat
      241 atccgagaca accacagcaa gttttgctgt gttgacgttc tggagatcct gccttacctg
      301 tcctgcctca cagctagtga ccaggatcga ctgcgggctt cctacaggca gatcgggaac
      361 cgggacacac tctggggact cttcaataat ctccagcgcc ggcctggctg ggtggaggtc
      421 ttcatccggg cactgcagat ctgtgagctg cctgggctgg ctgatcaagt gactcgagtt
      481 tatcagagct acctgcctcc ggggacctca ctccgctccc tagagccact gcagttacca
      541 gactttcctg ctgcggtttc tggaccctct gcatttgcgc caggtcacaa catccctgac
      601 catggcttac gagagacacc aagttgcccc aagcctgtcc aggacaccca gccaccagag
      661 tccccagtag agaattcaga gcaactcctc cagaccaact ccggggccgt cgcgaggatg
      721 tctggtggct ctttgatacc ctctcctaac cagcaggctc tcagccctca gccctccaga
      781 gagcatcaag agcaagaacc agaactgggt ggcgcccacg cagcaaatgt tgcctctgtt
      841 cccatagcaa cctatggacc tgtgtctcca accgtttcct tccagcccct tccacgtact
      901 gccctgagga caaacctctt gtctggggtc acagtatcag ccctatctgc tgatacctct
      961 ttgtcctcct cgtccactgg atcagctttt gcaaagggag ctggtgacca ggccaaagct
     1021 gccacctgtt tcagtactac actcaccaat tctgtgacta ccagctcagt gccttctccc
     1081 agattggtcc cagtaaaaac catgtcttcc aagttgcccc tcagttcaaa gtccactgct
     1141 gcgatgacgt ctactgtgct caccaataca gcgccatcaa aattacccag caactcagtg
     1201 tatgcgggca cagtgccatc cagagtgcct gctagtgtgg ccaaagcacc tgccaacaca
     1261 ataccacctg agaggaacag caagcaagcc aaggagaccc cggagggtcc agcaaccaaa
     1321 gtcaccactg gaggcaacca gactggacca aatagcagta tcaggagctt gcactctgga
     1381 ccagagatga gcaagccagg tgtgctggta tcccagttgg acgagccatt ctcagcctgc
     1441 tctgtggacc ttgccattag ccctagcagc tccttggtct cagaacccaa ccatggtcca
     1501 gaggagaatg agtattcgtc ctttagaatc caggtagacg aaagccccag tgctgatcta
     1561 ttaggaagcc ctgagccact agccacccag cagccccaag aagaggaaga acattgtgcc
     1621 agttcaatgc cctgggctaa gtggcttggg gccaccagtg cactcttggc tgtattcctg
     1681 gcagtgatgc tgtaccgtag taggcgcctg gcccagtgaa gcctcagctg tatgctgttc
     1741 tcttgctcag ttctgccaag catgttctct aggcttgggc tagtagaggc tgagtcagag
     1801 aaacttaaat atggcgaggt ccactgagct atccaggtag atagctacac caagacgtca
     1861 tcactgttgg gtgggggaga gacattgttt tatcctggtt catatgtcat cttctggtct
     1921 tcagcttttg gaggcactgt gttacctcca ttgctcctga cctgcccacg tggcagtgta
     1981 agagttcatg ctctgtgctc ctaaggaggt atctccacca gctttatccc tgttggccca
     2041 agcctgaaga tgaggaggta tcgcactgtt gacaatgccc ctcatctcgc ccagatcttc
     2101 tgaaacacaa gcgtaggtac actgatgagt gctcccatag catcttatgc cttccaccgg
     2161 cagcctggtc taaggccttg gcaggagagc ctgggagtaa cagagtggct ggtctctagg
     2221 gagctttgag tgctctgccc ctttgggccc tctccacagc cagtcaaccc tgagggtgag
     2281 tctggaggtt ctctgtggac tcttaagaaa gccagaagca actgccaggc ctgaggaagc
     2341 ccctgggagg gagcttttct ccaacctcct taccccctcc tcgcttctcc actcctggtc
     2401 tccattctct ccctatactg gatgatgaga tacctagtgg gcagaactca ggctgggctt
     2461 gcatcagttc caccctttcc ctggccttta gcagatgtct agcttctcta gtcttgttcc
     2521 caatgtagat agcgggcagc tcttcctttt actgggctcc tctgagtttg aaggatacag
     2581 atacttgcct agtacttatc tcagagggtg tgagtacaag ttcttgccct cctgccctgg
     2641 ctggctgagc taaggtgaga gtgtgagagg cagagtgcat attggtaatg gcagtctttg
     2701 agagagcagc cttcatctcc atcatgcttc tttctcctcc ctggtcaggt gtaggcctca
     2761 cctctgtgcc tcacttttct tacctgtgag atggggtgag tatgtgtgtg tatacacaca
     2821 catacccaca gacccacgcc cacacccaca catacatgct aatatattct cacctgtgag
     2881 atggaaagta tgcatacatt gatgctaata tattctagtt ttatcaggat aattaaaaga
     2941 gtttatctgt gcaaaaaaaa aaaaaaaaaa aaaaaagggc ggccgctcta gagtatccct
     3001 cgaggggccc aagcttacgc gtacccagct ttcttgtaca aagttggcat tataagaaag
     3061 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     3121 tgccatccag ctgcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     3181 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     3241 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
     3301 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     3361 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     3421 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     3481 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     3541 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     3601 tgatgcgctg gcagtgtccc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     3661 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     3721 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     3781 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     3841 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     3901 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     3961 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     4021 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     4081 ttattcagat tgggccccgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4141 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4201 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4261 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4321 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4381 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4441 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4501 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4561 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4621 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4681 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4741 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4801 tgcgttatcc cctgattctg tggataaccg tattaccgct agcatggatc tcggggacgt
     4861 ctaactacta agcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
     4921 gaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     4981 aatccgccgg gagcggattt gaacgttgtg aagcaacggc ccggagggtg gcgggcagga
     5041 cgcccgccat aaactgccag gcatcaaact aagcagaagg ccatcctgac ggatggcctt
     5101 tttgcgtttc tacaaactct tcctgttagt tagttactta agctcgggcc c
//
