LOCUS       sequence-314287-        9270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine Mavs in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Gstalder C, Liu D, Miao D, Lutterbach B, DeVine AL, Lin C, Shettigar
            M, Pancholi P, Buchbinder EI, Carter SL, Manos MP, Rojas-Rudilla V, 
            Brennick R, Gjini E, Chen PH, Lako A, Rodig S, Yoon CH, Freeman GJ, 
            Barbie DA, Hodi FS, Miles W, Van Allen EM, Haq R
  TITLE     Inactivation of Fbxw7 impairs dsRNA sensing and confers resistance 
            to PD-1 blockade.
  JOURNAL   Cancer Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416.
  PUBMED    32371478
REFERENCE   2  (bases 1 to 9270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416."
COMMENT     Sequence Label: pLX304_zeo_mmMavs_V5
FEATURES             Location/Qualifiers
     source          1..9270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..501
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     420..438
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             513..887
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    947..1064
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1181..1484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1485..1688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1639..1659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1711..1735
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             1747..1767
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(3305..3329)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3331..3372
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3414..4002
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3467..3487)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3885..3896)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4074..4307
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4385..4506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4422..4441)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4476..4495
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4546..4681
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4701..4720)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4702..4720)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4730..4747)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4730..4746)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4739..4761)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4888..5343
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4975..4994)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5185..5206
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5369..5473
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5474..5542,5543..6334)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5692..5711)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6505..7093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6994..7013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7247..7264
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7381..7402
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7417..7422,7423..7440,7441..7447)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7455..7471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7460..7482
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7479..7495
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7479..7495
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7514..7534
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7516..7534
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7562..7788
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7789..7969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8016..8141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8634..8867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9052..9096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
       61 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
      121 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
      181 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
      241 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
      301 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
      361 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
      421 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
      481 atcaccgacc tctctcccca gggggtacca ccatggccaa gttgaccagt gccgttccgg
      541 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
      601 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
      661 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
      721 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
      781 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
      841 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgactg cagctgcagt
      901 agtaagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      961 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1021 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     1081 acagcagaga tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa
     1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     1501 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     1561 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     1621 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     1681 gcagagctct ctggctaact gtcgggatca acaagtttgt acaaaaaagc aggctccgcg
     1741 gccgccttgt ttaactttaa gaaggagccc ttcaccgcca tgacatttgc tgaggacaag
     1801 acctataagt atatccgaga caaccacagc aagttttgct gtgttgacgt tctggagatc
     1861 ctgccttacc tgtcctgcct cacagctagt gaccaggatc gactgcgggc ttcctacagg
     1921 cagatcggga accgggacac actctgggga ctcttcaata atctccagcg ccggcctggc
     1981 tgggtggagg tcttcatccg ggcactgcag atctgtgagc tgcctgggct ggctgatcaa
     2041 gtgactcgag tttatcagag ctacctgcct ccggggacct cactccgctc cctagagcca
     2101 ctgcagttac cagactttcc tgctgcggtt tctggaccct ctgcatttgc gccaggtcac
     2161 aacatccctg accatggctt acgagagaca ccaagttgcc ccaagcctgt ccaggacacc
     2221 cagccaccag agtccccagt agagaattca gagcaactcc tccagaccaa ctccggggcc
     2281 gtcgcgagga tgtctggtgg ctctttgata ccctctccta accagcaggc tctcagccct
     2341 cagccctcca gagagcatca agagcaagaa ccagaactgg gtggcgccca cgcagcaaat
     2401 gttgcctctg ttcccatagc aacctatgga cctgtgtctc caaccgtttc cttccagccc
     2461 cttccacgta ctgccctgag gacaaacctc ttgtctgggg tcacagtatc agccctatct
     2521 gctgatacct ctttgtcctc ctcgtccact ggatcagctt ttgcaaaggg agctggtgac
     2581 caggccaaag ctgccacctg tttcagtact acactcacca attctgtgac taccagctca
     2641 gtgccttctc ccagattggt cccagtaaaa accatgtctt ccaagttgcc cctcagttca
     2701 aagtccactg ctgcgatgac gtctactgtg ctcaccaata cagcgccatc aaaattaccc
     2761 agcaactcag tgtatgcggg cacagtgcca tccagagtgc ctgctagtgt ggccaaagca
     2821 cctgccaaca caataccacc tgagaggaac agcaagcaag ccaaggagac cccggagggt
     2881 ccagcaacca aagtcaccac tggaggcaac cagactggac caaatagcag tatcaggagc
     2941 ttgcactctg gaccagagat gagcaagcca ggtgtgctgg tatcccagtt ggacgagcca
     3001 ttctcagcct gctctgtgga ccttgccatt agccctagca gctccttggt ctcagaaccc
     3061 aaccatggtc cagaggagaa tgagtattcg tcctttagaa tccaggtaga cgaaagcccc
     3121 agtgctgatc tattaggaag ccctgagcca ctagccaccc agcagcccca agaagaggaa
     3181 gaacattgtg ccagttcaat gccctgggct aagtggcttg gggccaccag tgcactcttg
     3241 gctgtattcc tggcagtgat gctgtaccgt agtaggcgcc tggcccagaa gggtgggcgc
     3301 gccgacccag ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt
     3361 ctcgattcta cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac
     3421 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3481 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3541 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3601 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3661 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3721 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3781 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     3841 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3901 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3961 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat
     4021 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     4081 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     4141 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     4201 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     4261 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag
     4321 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     4381 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4441 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4501 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     4561 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4621 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4681 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     4741 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     4801 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     4861 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     4921 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4981 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5041 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5101 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5161 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5221 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5281 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     5341 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5401 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5461 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5521 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5581 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5641 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5701 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5761 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5821 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5881 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5941 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6001 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6061 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6121 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6181 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6241 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6301 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6361 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6421 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6481 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcataa tctgctgctt
     6541 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6601 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6661 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6721 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6781 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6841 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6901 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6961 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7021 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7081 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7141 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7201 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7261 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7321 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     7381 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     7441 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     7501 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     7561 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     7621 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     7681 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     7741 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     7801 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7861 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7921 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     7981 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     8041 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     8101 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     8161 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     8221 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     8281 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     8341 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     8401 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     8461 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     8521 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     8581 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     8641 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     8701 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     8761 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     8821 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     8881 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     8941 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9001 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9061 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     9121 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     9181 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     9241 cagacccacc tcccaacccc gaggggaccc
//
