LOCUS       sequence-31431-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HERC2 (2KEO)
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             410..427
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(480..498)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      494..541
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(665..684)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aattccctct gaatattttg tttactttaa gaaggagata taccatgcat catcatcatc
       61 atcacagcag cggcagagaa aacttgtatt tccagggcaa taatgagaaa gtgacacttg
      121 tacgcatagc tgatttggag aaccataata acgatggagg cttctggact gtgattgacg
      181 ggaaagtgta tgatataaag gacttccaga cacagtcgtt aacagaaaat agtattcttg
      241 ctcagtttgc aggggaagac ccagtggtag ctttggaagc tgctttgcag tttgaagaca
      301 cccgggaatc catgcacgca ttttgtgttg gccagtattt ggagcctgac caagaaggcg
      361 tcaccatacc agatctgggg tgatgacgaa gcttgcggcc gcactcgagc accaccacca
      421 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      481 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      541 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      601 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      661 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      721 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      781 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gac
//
