LOCUS       sequence-314335-       10118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine Ddx58 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Gstalder C, Liu D, Miao D, Lutterbach B, DeVine AL, Lin C, Shettigar
            M, Pancholi P, Buchbinder EI, Carter SL, Manos MP, Rojas-Rudilla V, 
            Brennick R, Gjini E, Chen PH, Lako A, Rodig S, Yoon CH, Freeman GJ, 
            Barbie DA, Hodi FS, Miles W, Van Allen EM, Haq R
  TITLE     Inactivation of Fbxw7 impairs dsRNA sensing and confers resistance 
            to PD-1 blockade.
  JOURNAL   Cancer Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416.
  PUBMED    32371478
REFERENCE   2  (bases 1 to 10118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416."
COMMENT     Sequence Label: pLenti6.2_3xFLAG_V5_ mmDdx58_V5
FEATURES             Location/Qualifiers
     source          1..10118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             598..663
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             682..723
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    721..745
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3554..3578)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3620..3661
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        3694..4193
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3729..3752)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4126..4145
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        4207..4254
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4273..4671
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4758..4991
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5063..5184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5100..5119)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5154..5173
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5224..5359
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5379..5398)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5380..5398)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5408..5425)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5408..5424)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5417..5439)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5566..6021
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5653..5672)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5863..5884
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6047..6151
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6152..6220,6221..7012)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6370..6389)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7183..7771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7672..7691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7925..7942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8059..8080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8095..8100,8101..8118,8119..8125)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8133..8149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8138..8160
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8157..8173
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8157..8173
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8192..8212
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8194..8212
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8240..8466
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8467..8647
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8694..8819
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9312..9545
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9730..9774
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagaggatcc agccatggac
      601 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
      661 aagggctcgg gctcgacctc gggtaagcct atccctaacc ctctcctcgg tctcgattct
      721 acaagtttgt acaaaaaagc agaagggccg tcaaggccca ccatgacagc ggagcagcgg
      781 cagaatctgc aagcattcag agactatatc aagaagattc tggaccccac ctacatcctc
      841 agctacatga gttcctggct cgaggatgag gaggtgcagt acattcaggc tgagaagaac
      901 aacaagggcc caatggaagc tgcctcactc ttcctccagt acctgttgaa gctgcagtca
      961 gagggctggt tccaggcctt tttggatgcc ctgtaccatg caggttactg tggactttgt
     1021 gaagccatcg aaagttggga ctttcaaaaa attgaaaagt tagaggaaca cagattactt
     1081 ttaagacgtt tagaaccaga atttaaggcc acagttgatc caaatgatat cctttctgaa
     1141 ctatccgaat gtttgattaa tcaggaatgt gaagaaatca gacagatccg agacactaaa
     1201 gggagaatgg caggtgcgga gaagatggcc gaatgtctta tcagatccga caaggaaaac
     1261 tggccaaagg tcttgcaact tgctttggag aaagacaaca gcaagtttag tgaattgtgg
     1321 attgttgata aaggtttcaa aagggctgaa agcaaggctg atgaggatga tggagcggag
     1381 gcgtccagca tccagatttt cattcaggaa gagccagagt gtcagaatct cagtcagaat
     1441 cccgggcctc cttcagaagc gtcttctaat aatttacaca gcccattgaa accaagaaat
     1501 taccaactgg agcttgccct gcctgccaag aaagggaaaa atacaataat atgtgcccct
     1561 actggttgtg gaaaaacctt tgtgtcgctt cttatatgtg aacaccatct taaaaaattc
     1621 ccatgtggac aaaaagggaa agtggtcttc ttcgctaacc aaattcctgt ctatgagcag
     1681 caggcaactg tgttctcacg atattttgaa agacttgggt acaacattgc gagcatttct
     1741 ggggcaacat ctgatagcgt ctcagtgcag cacatcattg aagacaatga tatcatcatc
     1801 ctgacacccc agattcttgt gaacaatctc aacaacggag ccatcccctc gttgtctgtc
     1861 ttcactctga tgatatttga tgagtgtcat aacactagca aaaaccaccc atacaatcag
     1921 atcatgttca gatacctaga ccacaaactt ggagagtcac gggacccact gcctcaggtc
     1981 gttgggctga ctgcctccgt cggcgttgga gatgctaaga ccgcggagga agccatgcaa
     2041 catatctgta aactctgtgc cgccctggat gcctccgtga ttgccacagt cagagacaac
     2101 gttgcagaac tggaacaggt cgtttataag ccccagaaaa tttccaggaa agtggcatcc
     2161 cggacttcga acacgtttaa atgcatcatc tctcagctga tgaaggagac agagaagcta
     2221 gccaaggatg tctccgagga acttggaaag ctttttcaaa ttcaaaacag agaattcggc
     2281 acccagaaat atgaacagtg gattgtcggc gtccacaaag cgtgctcagt gtttcagatg
     2341 gcagacaaag aggaggagag ccgggtctgc aaagcgctct tcctgtacac atcacatttg
     2401 cggaaataca acgatgcact catcatcagt gaggatgcac agatgacaga cgctctaaat
     2461 tacctcaaag ccttcttcca cgatgtccga gaagcagcat tcgatgagac cgagcgagag
     2521 cttactcgga ggtttgaaga aaaactagag gaattagaaa aagtttccag ggatcccagc
     2581 aatgagaatc ctaaactaag agacctctac ttggtcttac aagaagagta ccacttaaag
     2641 ccagagacca agaccattct cttcgtgaag accagagcac tcgtggatgc tctgaagaaa
     2701 tggattgaag aaaatcctgc actaagcttt ctaaagcctg gcatactgac tgggcgtggc
     2761 agaacaaacc gggcaacagg aatgacgctc ccggcacaga agtgtgtgct ggaggcattc
     2821 agagccagcg gagataacaa tattctgatt gctacctcgg tcgctgatga aggcattgac
     2881 attgctgagt gcaatctcgt cattctctat gagtacgtgg gcaacgtcat caagatgatc
     2941 caaaccagag gccgaggaag agcacgagat agcaagtgct tcctcctgac cagcagcgct
     3001 gacgtgattg aaaaagaaaa ggcgaacatg atcaaggaaa aaataatgaa tgaatccatc
     3061 ttaagactgc agacatggga tgaaatgaaa tttggaaaga cggttcaccg catacaggtg
     3121 aatgaaaaac tcctcagaga cagtcagcac aaaccacaac ctgttcctga caaagaaaac
     3181 aagaaactgc tgtgtggaaa gtgcaagaat tttgcgtgct acacagctga cattcgagtg
     3241 gttgagacgt cccactacac tgtccttgga gacgctttta aggagcgctt tgtgtgtaag
     3301 ccacacccta aaccaaagat ctatgacaat tttgagaaga aagcaaagat attctgcgcc
     3361 aaacagaact gtagccacga ctggggaatt tttgtgagat acaagacgtt cgagattcca
     3421 gtcataaaaa ttgaaagttt cgtcgtggaa gatattgtga gcggagttca gaaccggcac
     3481 tcaaagtgga aggactttca ttttgaaagg atacagttcg atcctgcaga aatgtccgta
     3541 tagggcctca tgggcccagc tttcttgtac aaagtggttg ctctatagta actcgagtct
     3601 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     3661 gcgtaccggt tagtaatgac gatggtacct accgggtagg ggaggcgctt ttcccaaggc
     3721 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     3781 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     3841 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     3901 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     3961 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     4021 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     4081 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     4141 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgactctag
     4201 acacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     4261 aggaactaaa ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca
     4321 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc
     4381 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
     4441 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
     4501 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
     4561 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
     4621 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agcacaattc
     4681 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     4741 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     4801 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4861 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4921 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4981 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     5041 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     5101 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5161 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     5221 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5281 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     5341 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     5401 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     5461 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     5521 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     5581 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     5641 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5701 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5761 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5821 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5881 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     5941 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     6001 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     6061 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6121 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     6181 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     6241 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     6301 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     6361 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     6421 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     6481 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     6541 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     6601 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6661 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6721 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6781 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6841 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6901 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6961 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     7021 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     7081 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     7141 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7201 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7261 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7321 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7381 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7441 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7501 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7561 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7621 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7681 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7741 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7801 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7861 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7921 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     7981 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     8041 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     8101 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     8161 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     8221 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     8281 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     8341 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     8401 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     8461 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8521 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8581 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8641 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     8701 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     8761 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     8821 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     8881 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     8941 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     9001 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     9061 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     9121 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     9181 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     9241 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     9301 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     9361 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     9421 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     9481 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     9541 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     9601 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     9661 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     9721 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     9781 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     9841 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     9901 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     9961 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
    10021 tctcgacggt atcgatgtcg acgttaacgc tagcgactgg gtctgcgatg tcgacgttaa
    10081 cgctagcgac tgggtctgcg ataagcttgg gagttccg
//
