LOCUS       sequence-314338-       10445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine Ifih1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10445)
  AUTHORS   Gstalder C, Liu D, Miao D, Lutterbach B, DeVine AL, Lin C, Shettigar
            M, Pancholi P, Buchbinder EI, Carter SL, Manos MP, Rojas-Rudilla V, 
            Brennick R, Gjini E, Chen PH, Lako A, Rodig S, Yoon CH, Freeman GJ, 
            Barbie DA, Hodi FS, Miles W, Van Allen EM, Haq R
  TITLE     Inactivation of Fbxw7 impairs dsRNA sensing and confers resistance 
            to PD-1 blockade.
  JOURNAL   Cancer Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416.
  PUBMED    32371478
REFERENCE   2  (bases 1 to 10445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416."
COMMENT     Sequence Label: pLenti6.2_3xFLAG_V5_ mmIfih1_V5
FEATURES             Location/Qualifiers
     source          1..10445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             598..663
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             682..723
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    721..745
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             757..777
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(3881..3905)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3947..3988
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        4021..4520
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4056..4079)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4453..4472
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        4534..4581
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4600..4998
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5085..5318
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5390..5511
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5427..5446)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5481..5500
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5551..5686
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5706..5725)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5707..5725)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5735..5752)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5735..5751)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5744..5766)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5893..6348
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5980..5999)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6190..6211
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6374..6478
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6479..6547,6548..7339)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6697..6716)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7510..8098
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7999..8018
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8252..8269
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8386..8407
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8422..8427,8428..8445,8446..8452)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8460..8476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8465..8487
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8484..8500
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8484..8500
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8519..8539
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8521..8539
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8567..8793
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8794..8974
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9021..9146
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9639..9872
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10057..10101
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagaggatcc agccatggac
      601 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
      661 aagggctcgg gctcgacctc gggtaagcct atccctaacc ctctcctcgg tctcgattct
      721 acaagtttgt acaaaaaagc aggctccgcg gccgccttgt ttaactttaa gaaggagccc
      781 ttcaccatgt cgattgtctg ttctgcagag gacagcttca ggaatctcat cttattcttc
      841 aggcccaggc tgaaaatgta cattcaggtg gagccagtgc tggaccacct catctttctg
      901 tctgcagaaa ccaaagagca gattcttaaa aagatcaaca cctgtggtaa caccagcgcg
      961 gcagaactgc tgctgagcac cttggagcag ggacaatggc ctctgggatg gacgcagatg
     1021 ttcgtggagg ccctagagca cagtggcaat cccctagccg cgcgctatgt caaacccaca
     1081 ctcactgatc tgccctctcc ttcctctgag actgcccatg acgagtgtct ccacttgctg
     1141 accctcctcc agcccacttt ggtggacaaa cttctgatta acgatgtctt ggacacttgc
     1201 ttcgagaagg gactattgac agtcgaagac agaaatcgga tttctgctgc aggaaacagc
     1261 gggaatgagt caggtgtaag agagctgctg agaaggattg tgcagaagga aaactggttt
     1321 tctaccttcc tggatgttct gcgccaaact ggaaatgatg cactattcca agaactaaca
     1381 ggtggaggct gcccagaaga caacacagac ttggctaact cgtctcacag agatgggcct
     1441 gcagctaatg agtgtcttct gcctgctgtc gatgagtcaa gtctggagac agaggcctgg
     1501 aacgtagacg acatattacc agaggcttct tgtacagatt cgtctgtgac cacagaatca
     1561 gacacaagtt tggcagaagg aagtgtcagc tgcttcgatg aaagtcttgg acataacagc
     1621 aacatgggca gggattcagg caccatggga agtgattcag atgaaagtgt catccagaca
     1681 aaaagagtat cccccgagcc agaactgcag ctcaggcctt accaaatgga agtggcccaa
     1741 ccagctctag atgggaagaa tattattatc tgcctcccca cggggagtgg gaaaaccaga
     1801 gtggctgttt acatcaccaa agatcactta gacaagaaga agcaggcatc tgaatccggg
     1861 aaggttatcg ttcttgtcaa taaggtaatg ttagcagaac aacttttccg aaaagagttc
     1921 aacccatatt tgaagaaatg gtatcgaatt attggattaa gtggcgatac ccagctgaaa
     1981 atatcatttc cagaagttgt caaatcttac gatgttatta tcagcactgc tcaaatcctt
     2041 gaaaactccc tcttaaatct ggagagtgga gacgatgacg gtgtgcagct gtcagacttc
     2101 tctctcatta tcattgatga gtgccatcac accaacaagg aggcagtcta taacaacatc
     2161 atgagacgat atttgaagca gaagctgaga aacaatgacc tcaagaaaca aaacaaacca
     2221 gccattcccc tgccgcagat actaggactg acagcttcac ctggtgttgg agcagccaaa
     2281 aagcagtctg aggctgaaaa acatatttta aatatatgtg ccaatcttga tgcctttacc
     2341 attaaaacag tgaaagagaa tcttggtcaa ctcaaacacc aaataaagga accatgcaag
     2401 aaatttgtga ttgctgatga caccagagaa aatccattta aagagaaact tctagaaatt
     2461 atggcaagca ttcagactta ctgccaaaaa agtccaatgt cagattttgg aacccaacat
     2521 tatgagcagt gggccattca aatggagaaa aaagctgcta aagacggaaa tcgcaaagat
     2581 cgcgtctgtg cagaacattt gaggaagtac aacgaagccc tacaaatcaa cgacacgatc
     2641 cgaatgattg atgcatatag ccacctggag acattctaca ctgatgagaa agaaaagaag
     2701 ttcgcagtcc tcaatgacag cgacaagagt gatgacgagg ccagcagttg caatgaccaa
     2761 cttaagggcg atgtaaagaa atctttgaaa ctggacgaaa cggatgaatt tctcatgaat
     2821 ttgttctttg ataacaagaa aatgttgaaa aaactagctg aaaacccaaa atacgagaat
     2881 gaaaaactca ttaaattaag aaacacgata ctggaacaat tcacaaggtc tgaggagtcc
     2941 tcccgaggaa ttatttttac aaaaacacga cagagcacct acgcactttc ccagtggatc
     3001 atggaaaatg caaagtttgc ggaagttgga gtcaaagcgc atcacctgat tggcgcgggg
     3061 cacagcagtg aagtcaagcc catgactcag actgaacaaa aagaagtcat tagtaaattt
     3121 cgcactggcg aaataaatct gcttatcgct acgacggtgg cagaggaagg cctggatatc
     3181 aaagagtgca atattgttat tcgttatggc cttgtcacga acgagatagc catggtccag
     3241 gcccggggtc gagccagagc tgatgaaagc acgtatgtcc tggtcaccag cagtggctca
     3301 ggagttaccg aacgggagat tgttaatgat ttccgagaga agatgatgta taaagctatt
     3361 aaccgtgttc aaaacatgaa accagaggag tatgcacata agattttgga attgcaggtg
     3421 caaagtatcc tggaaaagaa aatgaaagtc aaaagaagca ttgcaaagca atacaacgac
     3481 aatccatcgt taataacact tctctgcaaa aattgtagca tgctggtctg ctcgggagaa
     3541 aacatccatg tcattgagaa gatgcatcat gtcaatatga caccagaatt caagggactc
     3601 tacattgtaa gagaaaacaa agcactgcaa aagaaatttg ctgattatca gaccaatgga
     3661 gagattatct gcaagtgtgg ccaggcttgg ggaacaatga tggtgcacaa aggtttagat
     3721 ttgccttgtc ttaaaataag gaattttgta gtcaatttca aaaataactc accgaagaaa
     3781 cagtacaaga agtgggtgga attgcctatc agatttcctg atcttgacta ctcagaatac
     3841 tgcttgtata gtgatgaaga ttttaagggt gggcgcgccg acccagcttt cttgtacaaa
     3901 gtggttgctc tatagtaact cgagtctaga gggcccgcgg ttcgaaggta agcctatccc
     3961 taaccctctc ctcggtctcg attctacgcg taccggttag taatgacgat ggtacctacc
     4021 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     4081 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     4141 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     4201 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     4261 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     4321 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     4381 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     4441 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     4501 cctcatctcc gggcctttcg actctagaca cgtgttgaca attaatcatc ggcatagtat
     4561 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagcc tttgtctcaa
     4621 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa
     4681 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat
     4741 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct
     4801 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg
     4861 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata
     4921 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt
     4981 tatgtgtggg agggctaagc acaattcgag ctcggtacct ttaagaccaa tgacttacaa
     5041 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5101 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5161 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5221 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5281 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     5341 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     5401 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5461 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5521 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5581 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5641 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     5701 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     5761 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5821 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     5881 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5941 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6001 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6061 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6121 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6181 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6241 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6301 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     6361 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6421 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6481 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6541 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6601 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6661 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6721 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6781 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6841 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6901 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6961 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7021 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7081 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7141 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7201 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7261 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7321 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7381 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7441 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7501 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7561 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7621 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7681 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7741 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7801 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7861 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7921 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7981 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8041 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8101 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8161 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8221 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8281 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     8341 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     8401 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     8461 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     8521 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     8581 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     8641 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     8701 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     8761 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     8821 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8881 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8941 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     9001 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     9061 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     9121 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     9181 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     9241 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     9301 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     9361 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     9421 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     9481 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     9541 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     9601 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     9661 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     9721 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     9781 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     9841 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     9901 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     9961 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    10021 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    10081 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    10141 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    10201 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    10261 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    10321 gacagatcca ttcgattagt gaacggatct cgacggtatc gatgtcgacg ttaacgctag
    10381 cgactgggtc tgcgatgtcg acgttaacgc tagcgactgg gtctgcgata agcttgggag
    10441 ttccg
//
